The Impact of Anti-tumor Agents on ER-Positive MCF-7 and HER2-Positive SKBR-3 Breast Cancer Cells Biomechanics

Metsiou, Despoina Nektaria; Siatis, Konstantinos E; Giannopoulou, Efstathia; Papachristou, Dionysios J.; Kalofonos, Haralabos; Koutras, Angelos; Athanassiou, G.

doi:10.1007/s10439-019-02284-3

Cited by 7 publications

(15 citation statements)

References 44 publications

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“…The created pores were 24.42 ± 3.01 μm in size, which was close to the diameter of tested cancer cells ( Figure 2 A). Notably, the average cell diameter of MCF-7 cells was measured 17.46 ± 1.92 μm ( Figure 2 B), and for SKBR-3 14.08 ± 1.55 μm ( Figure 2 C) [ 16 ]. The fiber diameter of PCL scaffold was found to be 1.26 ± 0.07 μm.…”

Section: Resultsmentioning

confidence: 99%

“…hUMSCs were isolated from the Wharton’s jelly of umbilical cords according to the protocol which analytically has been described in the study of Chatzistamatiou et al [ 15 ] complied to ethical standards and local ethics committee. Adenocarcinoma cell lines SKBR-3 and MCF-7 purchased from ATCC (USA) were cultured as in previous studies [ 16 ]. Briefly, DMEM supplemented with 2 mM L-glutamine was used as a culture medium.…”

Section: Methodsmentioning

confidence: 99%

“…More specifically, only the cell membrane was aspirated, thus avoiding nucleus aspiration, in order to obtain pure measurements of the cell membrane for the applied negative pressure at each time point. The experimental setup, as described before [ 16 , 17 ], consists of a borosilicate glass capillary micropipette, controlled by a mechanical micromanipulator, an inverted microscope with a camera, an adjustable fluid reservoir for creating fluid pressure in the micropipette, a pressure transducer, a monitor for digital display and a software. A range of pressure

from 0.05 to 340 Pa was applied, to obtain a linear expression of cell deformation vs. aspiration pressure.…”

Section: Methodsmentioning

confidence: 99%

“…When the micropipette radius was very small compared to the local radius of the cell surface, the projection of cell length,

, into micropipette, was predicted to be proportional to the aspiration pressure

. Therefore, cancer cell elasticity was determined through the slope of the curve:

via the interpreted equation:

where all variables have been thoroughly described in our previous work [ 16 ]. Briefly,

is the elastic Young’s modulus,

the inner diameter of micropipette

the inner pipette radius and

represents a function of the ratio of the pipette wall thickness to the pipette radius (

= 2.0−2.1 when the ratio of the pipette wall thickness to radius is equal to 0.2–1.0).…”

Section: Methodsmentioning

confidence: 99%

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Engineering Breast Cancer Cells and hUMSCs Microenvironment in 2D and 3D Scaffolds: A Mechanical Study Approach of Stem Cells in Anticancer Therapy

2021

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Cell biomechanics plays a major role as a promising biomarker for early cancer diagnosis and prognosis. In the present study, alterations in modulus of elasticity, cell membrane roughness, and migratory potential of MCF-7 (ER+) and SKBR-3 (HER2+) cancer cells were elucidated prior to and post treatment with conditioned medium from human umbilical mesenchymal stem cells (hUMSCs-CM) during static and dynamic cell culture. Moreover, the therapeutic potency of hUMSCs-CM on cancer cell’s viability, migratory potential, and F-actin quantified intensity was addressed in 2D surfaces and 3D scaffolds. Interestingly, alterations in ER+ cancer cells showed a positive effect of treatment upon limiting cell viability, motility, and potential for migration. Moreover, increased post treatment cell stiffness indicated rigid cancer cells with confined cell movement and cytoskeletal alterations with restricted lamellipodia formation, which enhanced these results. On the contrary, the cell viability and the migratory potential were not confined post treatment with hUMSCs-CM on HER2+ cells, possibly due to their intrinsic aggressiveness. The increased post treatment cell viability and the decreased cell stiffness indicated an increased potency for cell movement. Hence, the therapy had no efficacy on HER2+ cells.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

from 0.05 to 340 Pa was applied, to obtain a linear expression of cell deformation vs. aspiration pressure.…”

Section: Methodsmentioning

confidence: 99%

“…When the micropipette radius was very small compared to the local radius of the cell surface, the projection of cell length,

, into micropipette, was predicted to be proportional to the aspiration pressure

. Therefore, cancer cell elasticity was determined through the slope of the curve:

via the interpreted equation:

where all variables have been thoroughly described in our previous work [ 16 ]. Briefly,

is the elastic Young’s modulus,

the inner diameter of micropipette

the inner pipette radius and

represents a function of the ratio of the pipette wall thickness to the pipette radius (

= 2.0−2.1 when the ratio of the pipette wall thickness to radius is equal to 0.2–1.0).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Engineering Breast Cancer Cells and hUMSCs Microenvironment in 2D and 3D Scaffolds: A Mechanical Study Approach of Stem Cells in Anticancer Therapy

2021

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“…The diversity in engineering applications and techniques being developed to uniquely address health issues affecting women over their lifespan is increasing at a fast pace. Building upon recent publications in the Annals of Biomedical Engineering related to women's health, 3,4,7,9,13,16,17 this special issue will expand on the biomechanics of reproductive organs and their supporting structures, structural organization of pelvic organs and tissues and their functional alterations with aging and prolapse, and female-related cancers.…”

mentioning

confidence: 99%