SummaryStudy aim: Several sprint interval training applications with different slope angles in the literature mostly focused on sprint running time and kinematic and dynamic properties of running. There is a lack of comparative studies investigating aerobic and anaerobic power. Therefore, this study aimed to examine the effects of sprint interval training on sloping surfaces on anaerobic and aerobic power.Material and methods: A total of 34 male recreationally active men aged 20.26 ± 1.68 years and having a BMI of 21.77 ± 1.74 were assigned to one of the five groups as control (CON), uphill training (EXP1), downhill training (EXP2), uphill + downhill training (EXP3) and horizontal running training (EXP4) groups. Gradually increased sprint interval training was performed on horizontal and sloping surfaces with an angle of 4°. The training period continued for three days a week for eight weeks. The initial and the final aerobic power was measured by an oxygen analyser and anaerobic power was calculated from the results of the Margaria-Kalamen staircase test.Results: Following the training programme, an increase in aerobic power was found in all training groups (EXP1 = 20.79%, EXP2 = 14.95%, EXP3 = 26.85%, p < 0.01) and EXP4 = 20.46%) (p < 0.05) in comparison with the CON group (0.12%), but there were no differences among the training groups. However, significant increases in anaerobic power were found in uphill training (4.91%) and uphill + downhill training (8.35%) groups (p < 0.05).Conclusion: This study showed that all sprint interval studies on horizontal and sloping surfaces have a positive effect on aerobic power, and uphill and combined training are the most effective methods for the improvement of anaerobic power.