In this study, the effects of cotton (CO) and polyester (PES) fabric support modules on the filtration performance of Chitosan/silver nanoparticles/graphene oxide (CS/AgNP/GO) composite membranes modified with Dimethyl acetamide (DMAc) were investigated. The contact angle of the modified CS matrix decreased with proper blending of membrane composites, which improved fluid transport due to strongly bound water oxygen-containing functional groups present in the modified chitosan, according to the FTIR report. The experimental results revealed that both plain CO and PES fabrics can successfully serve as support modules for 3D printed CS/AgNP/GO composite membranes, increase water permeability, and effectively improve the filtration process. However, the effectiveness of the modified CS membrane separation process depends on the molecular interaction between the membrane composite structure and the support materials. The water absorbency of CO fabric is 16.3% higher than that of polyester (PES), confirming greater wettability. The CO-supported composite membranes exhibited the highest average permeation flux of 0.221 Lm− 2h− 1, while that of the PES-supported composite and unsupported modified CS membranes was 0.153 Lm− 2h− 1 and 0.127 Lm− 2h− 1, respectively, indicating that 100% plain CO and PES fabrics may serve as membrane support materials for water purification. Furthermore, the presence of particulate matter, including inactivated or active bacterial strains, on the membrane surface and low pressure could influence a reduction in permeation flux.