Maintaining the proper indoor microclimate and the correct level of thermal comfort is extremely important due to the impact of the surrounding environment on people's satisfaction, well-being, health, and work. This problem is particularly important in rooms where children spend long periods as the environment has a stronger influence on their still-developing organisms. The study assessed the microclimate parameters, the state of human thermal comfort, and the level of carbon dioxide concentration, as well as the maintained levels of comfort and air quality after the application of improvements aimed at rationalizing heat consumption in educational buildings. The results of basic thermal and personal microclimate parameter measurements, as well as carbon dioxide levels, were presented. The assessment of human thermal comfort was based on measured values, the Predicted Mean Vote, and Predicted Percentage Dissatisfied. The average rating of thermal comfort in the observed environment was within the acceptable comfort zone, but high concentrations of carbon dioxide indicates low efficiency of the ventilation system.