The impact of different intensities of green light on the bacteriochlorophyll homologue composition of the chlorobiaceae Prosthecochloris aestuarii and Chlorobium phaeobacteroides

Massé, Astrid; Airs, Ruth L.; Keely, Brendan J.; Wit, Rutger de

doi:10.1099/mic.0.27048-0

Cited by 10 publications

(18 citation statements)

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“…In this respect, "Ca. Chloracidobacterium thermophilum" resembles the GSB C. phaeobacteroides or green FAPs, such as Chloronema sp., which similarly display a diversity of methylation patterns and esterifying alcohols, including unbranched saturated alcohols (25,42,50). In both "Ca.…”

Section: Discussionmentioning

confidence: 99%

“…Another source of diversity in the BChls in chlorosomes is the esterifying alcohol of the C-17 propionic acid side chain. The majority (Ͼ95%) of the BChl c molecules in most GSB are esterified with the C-15 isoprenoid farnesol (12,50,70). However, BChl c molecules in FAPS are esterified with alcohols that include both isoprenoid and nonisoprenoid alkyl chains (5,26,27,41,43,45).…”

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confidence: 99%

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Identification of the Bacteriochlorophylls, Carotenoids, Quinones, Lipids, and Hopanoids of "Candidatus Chloracidobacterium thermophilum"

Costas

Tsukatani

Rijpstra

et al. 2011

Journal of Bacteriology

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"Candidatus Chloracidobacterium thermophilum" is a recently discovered chlorophototroph from the bacterial phylum Acidobacteria, which synthesizes bacteriochlorophyll (BChl) c and chlorosomes like members of the green sulfur bacteria (GSB) and the green filamentous anoxygenic phototrophs (FAPs). The pigments (BChl c homologs and carotenoids), quinones, lipids, and hopanoids of cells and chlorosomes of this new chlorophototroph were characterized in this study. "Ca. Chloracidobacterium thermophilum" methylates its antenna BChls at the C-8 2 and C-12 1 positions like GSB, but these BChls were esterified with a variety of isoprenoid and straight-chain alkyl alcohols as in FAPs. Unlike the chlorosomes of other green bacteria, "Ca. Chloracidobacterium thermophilum" chlorosomes contained two major xanthophyll carotenoids, echinenone and canthaxanthin. These carotenoids may confer enhanced protection against reactive oxygen species and could represent a specific adaptation to the highly oxic natural environment in which "Ca. Chloracidobacterium thermophilum" occurs. Dihydrogenated menaquinone-8 [menaquinone-8(H 2 )], which probably acts as a quencher of energy transfer under oxic conditions, was an abundant component of both cells and chlorosomes of "Ca. Chloracidobacterium thermophilum." The betaine lipid diacylglycerylhydroxymethyl-N,N,N-trimethyl-␤-alanine, esterified with 13-methyl-tetradecanoic (isopentadecanoic) acid, was a prominent polar lipid in the membranes of both "Ca. Chloracidobacterium thermophilum" cells and chlorosomes. This lipid may represent a specific adaptive response to chronic phosphorus limitation in the mats. Finally, three hopanoids, diploptene, bacteriohopanetetrol, and bacteriohopanetetrol cyclitol ether, which may help to stabilize membranes during diel shifts in pH and other physicochemical conditions in the mats, were detected in the membranes of "Ca. Chloracidobacterium thermophilum."

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Identification of the Bacteriochlorophylls, Carotenoids, Quinones, Lipids, and Hopanoids of "Candidatus Chloracidobacterium thermophilum"

Costas

Tsukatani

Rijpstra

et al. 2011

Journal of Bacteriology

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“…Based on the m/z molecular ions of these isomers (821.5, 835.5 and 849.5) and their respective fragment ions (through the loss of H 2 O molecule; −18 AMU) in each spectrum (Fig. 4b, c and d) and published literature, these were identified as farnesyl-esterified bacteriochlorophyll (BChl e F ) homologues such as e2 (Et, Et), e3 (n-Pr, Et) and e4 (i-Bu, Et) (Airs et al, 2001b;Glaeser et al, 2002, Massé et al, 2004. BChl e2 varied from below detection limit (BDL) to 2 µg l −1 with a mean concentration of 0.06 µg l −1 .…”

Section: Phytoplankton Pigmentsmentioning

confidence: 99%

“…This phenomenon is explained as a result of changing availability of reducing power, i.e. the highly reduced straight-chain alcohols have a higher biosynthetic demand for NADPH 2 than the polyunsaturated isoprenoid with the same number of carbon atoms (Massé et al, 2004). In the present study, we found the dominant bacteriochlorophylls to be farnesyl esterified (BChl e F ) isomers based on their MS spectra, in comparison with the standards (extracted from Salt Pond, USA), and previous literature.…”

Section: Anoxygenic Photosynthesis In Tillari and Selaulim Reservoirsmentioning

confidence: 99%

“…Although farnesol is the main alcohol esterifying the bacteriochlorophylls at position C-17, earlier studies have shown that a number of other alcohols are present in natural samples (Caple et al, 1978;Otte et al, 1993) depending on growth conditions, light intensity and stage of development of the cell (Borrego and Garcia-Gil, 1995;Airs et al, 2001b). Massé et al (2004) reported that in cultured Cb. phaeobacteroides, the isoprenoid esterified side chains dominated at low light intensities, while the straight-chain alkyl substituents dominated at higher light intensities.…”

Section: Anoxygenic Photosynthesis In Tillari and Selaulim Reservoirsmentioning

confidence: 99%

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Seasonal occurrence of anoxygenic photosynthesis in Tillari and Selaulim reservoirs, Western India

et al. 2012

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Abstract. Phytoplankton and bacterial pigment compositions were determined by high performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) in two freshwater reservoirs (Tillari Dam and Selaulim Dam), which are located at the foothills of the Western Ghats in India. These reservoirs experience anoxia in the hypolimnion during summer. Water samples were collected from both reservoirs during anoxic periods while one of them (Tillari Reservoir) was also sampled in winter, when convective mixing results in well-oxygenated conditions throughout the water column. During the period of anoxia (summer), bacteriochlorophyll (BChl) e isomers and isorenieratene, characteristic of brown sulfur bacteria, were dominant in the anoxic (sulfidic) layer of the Tillari Reservoir under low light intensities. The winter observations showed the dominance of small cells of Chlorophyll b-containing green algae and cyanobacteria, with minor presence of fucoxanthin-containing diatoms and peridinin-containing dinoflagellates. Using total BChl e concentration observed in June, the standing stock of brown sulfur bacteria carbon in the anoxic compartment of Tillari Reservoir was estimated to be 2.27 gC m−2, which is much higher than the similar estimate for carbon derived from oxygenic photosynthesis (0.82 gC m−2. The Selaulim Reservoir also displayed similar characteristics with the presence of BChl e isomers and isorenieratene in the anoxic hypolimnion during summer. Although sulfidic conditions prevailed in the water column below the thermocline, the occurrence of photo-autotrophic bacteria was restricted only to mid-depths (maximal concentration of BChl e isomers was detected at 0.2% of the surface incident light). This shows that the vertical distribution of photo-autotrophic sulfur bacteria is primarily controlled by light penetration in the water column where the presence of H2S provides a suitable biogeochemical environment for them to flourish.

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A multi‐tracers analysis of sources and transfers of particulate organic matter in a tropical reservoir (Petit Saut, French Guiana)

Junet

Abril

Guerin

et al. 2008

River Research & Apps

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We measured the organic carbon (OC) content, the isotopic composition, the C/N ratios and the photosynthetic pigment composition of suspended matter, sediments, sediments traps and epiphytic and epilithic biofilms at the tropical Sinnamary River system (French Guiana). Our sampling included the mid-stream reservoir lake (Petit Saut) and the estuary on the Atlantic coast. These tracers were complementary and allowed identifying different sources of particulate organic matter (POM) in the system. We found a d 13 C-C/N signature of POM close to that of soils and litters collected in the surrounding forest, both for water column and sediment at the upstream station as well as for the sediment in a littoral zone of the reservoir, which thus indicated a terrestrial origin. Plankton communities at the centre of the reservoir were dominated by Chlorophyceae (chlorophyll a (Chl a), chlorophyll b (Chl b) and lutein) in the oxic epilimnion and by anoxygenic phototrophic bacteria, namely Chlorobiaceae (bacteriochlorophyll d (BChl d) and bacteriochlorophyll c (BChl c)) at and below the oxycline (6 m depth). In addition, this planktonic material was slightly 13 C-depleted due to a contribution of methanotrophic bacteria. Phytoplankton and bacterioplankton were the major source of settling material collected in the traps at all depths in the centre of the reservoir. In the traps, POM was subject to intense degradation, as revealed by C/N and isotopic data and by the presence of pheopigments. In the river downstream of the dam, Chl b, lutein, BChl c and d originating from the reservoir progressively decreased downstream as the result of mineralization. At the estuarine mouth, fucoxanthin showed the presence of diatoms and the d 13 C-C/N signature matched that of POM carried by the Amazonian coastal mobile mud belt. By analysing sedimentation rates in the reservoir and its outflow into the river, we were able to provide a first estimates of POM transfers in this system during the sampling period. POM IN A TROPICAL RESERVOIR 255 Figure 2. Typical chromatograms at 440 and 664 nm showing most of the pigments quantified in the Petit Saut samples. (A) Surface sediment at Station 1; (B) suspended matter at À6 m depth at Station 4 A. DE JUNET ET AL. Ã Indicates that lutein and BChl d coeluted in the samples and lutein concentrations were corrected from the BChl c and d concentration as detailed in the text.

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The impact of different intensities of green light on the bacteriochlorophyll homologue composition of the chlorobiaceae Prosthecochloris aestuarii and Chlorobium phaeobacteroides

Cited by 10 publications

References 33 publications

Identification of the Bacteriochlorophylls, Carotenoids, Quinones, Lipids, and Hopanoids of "Candidatus Chloracidobacterium thermophilum"

Identification of the Bacteriochlorophylls, Carotenoids, Quinones, Lipids, and Hopanoids of "Candidatus Chloracidobacterium thermophilum"

Seasonal occurrence of anoxygenic photosynthesis in Tillari and Selaulim reservoirs, Western India

A multi‐tracers analysis of sources and transfers of particulate organic matter in a tropical reservoir (Petit Saut, French Guiana)

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