The impact of different nanoparticle surface chemistry and size on uptake and toxicity in a murine macrophage cell line

Aim: To investigate the influence of gold nanoparticle (GNP) geometry on the biochemical response of Calu-3 epithelial cells. Materials and Methods:Spherical, triangular and hexagonal GNPs were used. The GNP-cell interaction was assessed via atomic absorption spectroscopy (AAS) and transmission electron microscopy (TEM). The biochemical impact of GNPs was determined over 72hrs at [0.0001-1mg/mL].Results: At 1mg/mL, hexagonal GNPs reduced Calu-3 viability below 60%, showed increased reactive oxygen species production and higher expression of pro-apoptotic markers. A cell mass burden of 1:2:12 as well as number of GNPs per cell (2:1:3) was observed for spherical:triangular:hexagonal GNPs. Conclusion:These findings do not suggest a direct shape-toxicity effect. However, do highlight the contribution of shape towards the GNP-cell interaction which impacts upon their intracellular number, mass and volume dose.

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“…Nonetheless, prediction of biocompatibility based on NP shape and dimensions is extremely challenging [34][35][36].…”

Section: Discussionmentioning

confidence: 99%

Investigating the Role of Shape on the Biological Impact of Gold Nanoparticles in Vitro

Tian

Clift

Casey³

et al. 2015

Nanomedicine (Lond.)

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“…We investigated how the physicochemical properties of released fragments from a n-CuPc containing automobile coating changed in biological (cell culture) media, and identified the hazard of the released n-CuPc based on the use of an in vitro macrophage model (J774 A1) (Yen et al, 2009;Clift et al, 2008Clift et al, , 2010Clift et al, , 2011. Fragments containing n-CuPc from automobile coatings (Frag n-CuPc), generated through a sanding approach, were compared with reference fragments without n-CuPc (Frag Refer) and pristine n-CuPc.…”

Section: Introductionmentioning

confidence: 99%

Releases from transparent blue automobile coatings containing nanoscale copper phthalocyanine and their effects on J774 A1 macrophages

et al. 2017

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A B S T R A C TNanoscale copper phthalocyanine (n-CuPc) is a pigment widely used in paints to enhance automobile coatings and to make colors transparent and more appealing to users. Despite the benefits, n-CuPc can potentially be released into the environment and pose risks in occupational settings. Assessing the toxicity of released n-CuPccontaining fragments is thus important for the acceptance of n-CuPc products. This paper presents the first combined study addressing both the release of n-CuPc-containing fragments from commercial coatings in realistic occupational situations and the hazard of the released fragments using a macrophage model.Sanding was used to produce fragments from automobile coatings with n-CuPc as well as from a reference coating with the same matrix composition but without n-CuPc. Size distribution and agglomeration of these fragments in Rosewell Park Memorial Institute (RPMI) cell culture medium was studied before conducting a battery of cytotoxicity experiments. Cell viability and reactive oxygen species (ROS) production were conducted and particle localization within cells was analyzed.The results show similar size and number distribution of fragments when comparing the reference and n-CuPc fragments. The n-CuPc fragments showed higher agglomeration in RPMI than pristine n-CuPc. We found that toxicity of the n-CuPc fragments and reference materials was similar (EC 50 Frag n-CuPc = 242.9 μg ml − 1 ; EC 50Frag Refer = 241.6 μg ml − 1 ) and below the toxicity of pristine n-CuPc (EC 50 n-CuPc = 151.1 μg ml). The results demonstrated that embedding n-CuPc in matrix used in automobile coatings reduced the toxicity and release when n-CuPc is used in paints. Our finding can be used to support design of n-CuPc-enabled products used in automobile applications.

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“…Without controlling the locations of particles, useful information is still gained, where particles uptaken by normal pathways in the cell can function as a type of nano-endoscope 3 , but there is significant interest in methods to control the uptake pathways and final destinations of nanoparticles in cells. Typical methods involve using conjugate tags with the particles, injecting the particles by micropipette, attaching particles to a nanopipette 5 or optimizing particle coatings to modulate typical cell-particle interaction pathways 6,7 . However, there are several limitations with these methods; bare nanoparticles do not readily penetrate cellular membranes, and even once inside the cell, they cannot move freely.…”

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confidence: 99%

Laser-targeted photofabrication of gold nanoparticles inside cells

et al. 2014

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Nanoparticle manipulation is of increasing interest, since they can report single moleculelevel measurements of the cellular environment. Until now, however, intracellular nanoparticle locations have been essentially uncontrollable. Here we show that by infusing a gold ion solution, focused laser light-induced photoreduction allows in situ fabrication of gold nanoparticles at precise locations. The resulting particles are pure gold nanocrystals, distributed throughout the laser focus at sizes ranging from 2 to 20 nm, and remain in place even after removing the gold solution. We demonstrate the spatial control by scanning a laser beam to write characters in gold inside a cell. Plasmonically enhanced molecular signals could be detected from nanoparticles, allowing their use as nano-chemical probes at targeted locations inside the cell, with intracellular molecular feedback. Such light-based control of the intracellular particle generation reaction also offers avenues for in situ plasmonic device creation in organic targets, and may eventually link optical and electron microscopy.

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The impact of different nanoparticle surface chemistry and size on uptake and toxicity in a murine macrophage cell line

Cited by 320 publications

References 40 publications

Investigating the Role of Shape on the Biological Impact of Gold Nanoparticles in Vitro

Investigating the Role of Shape on the Biological Impact of Gold Nanoparticles in Vitro

Releases from transparent blue automobile coatings containing nanoscale copper phthalocyanine and their effects on J774 A1 macrophages

Laser-targeted photofabrication of gold nanoparticles inside cells

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