The impact of feeding β<sub>2</sub>‐adrenergic agonists on rainbow trout muscle β<sub>2</sub>‐adrenoceptors and protein synthesis

Lortie, Michel B.; Arnason, Trevor; Dugan, Stephen G.; Nickerson, James G.; Moon, Thomas W.

doi:10.1111/j.0022-1112.2004.00482.x

Cited by 8 publications

(5 citation statements)

References 49 publications

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“…Several studies support these findings using total protein or myofibrillar fractions. Anabolism induced by the β2-adrenergic agonist, ractopamine, which is orally administered to fish to promote muscle accretion, was found to significantly increase MyHC mRNA (Salem et al ., 2006) and myofibrillar protein (Lortie et al ., 2004). Hevrøy et al .…”

Section: Discussionmentioning

confidence: 99%

Characterization and expression of a myosin heavy–chain isoform in juvenile walleye Sander vitreus

Dhillon

Esbaugh

Wang

et al. 2009

Journal of Fish Biology

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In this study, myosin, the major component of myofibrillar protein in the skeletal muscle, was characterized and its expression was monitored during growth in juvenile walleye Sander vitreus. First, the coding region of myosin heavy chain (MyHC) from the fast skeletal muscle of walleye was amplified by long-distance PCR using a full-length cDNA. Phylogenetic analysis was used to determine the evolutionary relationship of this S. vitreus myosin sequence to other vertebrate myosin sequences. Next, it was established that the myosin isoform was most prevalent in the white muscle, compared with the red and cardiac muscle. Myosin expression was monitored over a series of experiments designed to influence growth. Specifically, change in MyHC mRNA was monitored after acute changes in feeding. Fish exposed to a one-week fasting period showed significant decreases in MyHC mRNA levels by the end of the fast. The effect of feeding was also examined more closely over a 24 h period after feeding, but results showed no significant change in myosin expression levels through this time period. Finally, fish with higher growth rates had higher MyHC mRNA and protein expression levels. This study indicates that MyHC mRNA expression is sensitive to the factors that may influence growth in juvenile S. vitreus.

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Section: Discussionmentioning

confidence: 99%

Characterization and expression of a myosin heavy–chain isoform in juvenile walleye Sander vitreus

Dhillon

Esbaugh

Wang

et al. 2009

Journal of Fish Biology

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“…It may have an anabolic impact on protein metabolism, causing muscle fiber growth and frequent changes in muscle fiber type [12]. Ractopamine has been tested in a few species of fish, such as channel catfish [13,14], rainbow trout [15][16][17][18][19], pacu [20,21], Hungarian carp [22], tilapia [5], and calbasu [23], with both positive and contradictory results. The objective of this study was thus to evaluate the effect of ractopamine as a dietary additive on the growth performance and gonadal maturity of all-female prawns.…”

Section: Introductionmentioning

confidence: 99%

Beta-Agonist, Ractopamine Hydrochloride, Improves Growth, Alters Body Composition, and Suppresses Gonadal Maturation in All-Female Giant Freshwater Prawn, Macrobrachium rosenbergii

Rahman

Salin

Tsusaka

et al. 2022

Aquaculture Nutrition

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All-female giant freshwater prawn Macrobrachium rosenbergii has a significant growth limitation due to its early sexual maturity. A 60-day trial was carried out to determine the effect of beta-agonist, ractopamine hydrochloride (RAC), on the growth performance, body composition, and gonadal maturation of all-female prawns. Prawn juveniles ( 4.39 ± 0.40 g initial weight) were stocked at 10 prawns/m2 in 15 plastic tanks (1000 liters each) in a completely randomized design with four treatments and a control (0, 5, 10, 15, and 20 mg/kg) in triplicate. The significantly ( p < 0.05 ) highest values of final weight, weight gain, daily weight gain, and specific growth rate (SGR) were observed in the female prawns fed with the diet containing 10 mg/kg RAC, while the lowest values were found in the control tank. Similarly, survival rate, feed conversion rate (FCR), and condition factors were observed among all treatments, which were significantly better than the control (0 mg/kg). Significantly greater yield and lower gonadosomatic index (GSI) values were obtained in prawns fed with a 10 mg/kg RAC-containing diet than in all other treatments, except for the diet containing 15 mg/kg RAC, while the lowest yield was produced by the control tank. A diet containing 10 mg/kg RAC had significantly improved the whole-body protein content in prawns, while diets containing 10 and 15 mg/kg RAC exhibited lower lipid content in prawns than in the other treatments. A significant increase in the number of Stage 1 (virgin) females (up to 54% at 10 mg/kg RAC diet) was found in all RAC treatments, while the control had a significantly higher number of females at various maturity stages, indicating that RAC administration effectively suppresses gonadal maturity. Residue analysis of whole prawn samples at the end of the trial indicated no trace of ractopamine. The quadratic regression analysis of weight gain, SGR, FCR, and yield revealed that the optimum dietary ractopamine level for all-female prawn culture should be between 10 and 15 mg/kg.

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“…One possible explanation for the high K d value measured for β-adrenoreceptors of the flounder heart is that they are functionally uncoupled from adenylate cyclase (Hausdorff et al, 1990). However, we feel that this is unlikely as resting levels of circulating catecholamines in the flounder are typical of those measured in other teleosts, the repeated injection of catecholamines into rainbow trout does not alter cardiac β-adrenoreceptor affinity (Gamperl et al, 1994a) and exposure to β-adrenoreceptor agonists for more than a month only resulted in an increase in the K d of rainbow trout muscle β-adrenoreceptors of 2-fold [from ~0.2 to 0.5 nmol l -1 (Lortie et al, 2004)]. A more probable explanation for the high K d value reported here for flounder cardiac β-adrenoreceptors is that the flounder heart has a significant population of β 3 -adrenoreceptors.…”

Section: Catecholamine Dose-response Curves and Cardiac β-Adrenorecepmentioning

confidence: 98%

Nervous and humoral control of cardiac performance in the winter flounder(Pleuronectes americanus)

Mendonça

Gamperl

2009

Journal of Experimental Biology

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SUMMARYPrevious studies have suggested that flatfish lack adrenergic cardiac innervation and have a limited humoral adrenergic stress response. However, data on neurohormonal control of flatfish cardiac function is scarce, and has never been directly studied in vivo. Hence, we (1) injected neural and humoral antagonists into flounder (Pleuronectes americanus) in vivo to determine the contribution of autonomic innervation and circulating catecholamines to the control of resting cardiac function; (2) measured preand post-stress (90 s chase) catecholamine levels in this species; and (3) constructed in vivo catecholamine dose-response curves for cardiovascular function based on the results of the second experiment. In addition, we quantified the density (B max ) and ligand-binding affinity (K d ) of flounder ventricular cell-surface β-adrenoreceptors, and established whether they were of β 1 or β 2 subtype using pharmacological antagonists. The cholinergic contribution to resting flounder heart rate was comparable to other teleosts (cholinergic tonus 26%). Interestingly, however, bretylium increased heart rate, resulting in a negative resting adrenergic tonus (-11.9%), and we were unable to demonstrate that catecholamines supported cardiac function at rest or at circulating concentrations approximating those following an exhaustive chase (adrenaline, 21 nmol l ) for these receptors raises the possibility that the flounder heart is also populated by β 3 -adrenoreceptors.

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The impact of feeding β₂‐adrenergic agonists on rainbow trout muscle β₂‐adrenoceptors and protein synthesis

Cited by 8 publications

References 49 publications

Characterization and expression of a myosin heavy–chain isoform in juvenile walleye Sander vitreus

Characterization and expression of a myosin heavy–chain isoform in juvenile walleye Sander vitreus

Beta-Agonist, Ractopamine Hydrochloride, Improves Growth, Alters Body Composition, and Suppresses Gonadal Maturation in All-Female Giant Freshwater Prawn, Macrobrachium rosenbergii

Nervous and humoral control of cardiac performance in the winter flounder(Pleuronectes americanus)

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The impact of feeding β2‐adrenergic agonists on rainbow trout muscle β2‐adrenoceptors and protein synthesis

Cited by 8 publications

References 49 publications

Characterization and expression of a myosin heavy–chain isoform in juvenile walleye Sander vitreus

Characterization and expression of a myosin heavy–chain isoform in juvenile walleye Sander vitreus

Beta-Agonist, Ractopamine Hydrochloride, Improves Growth, Alters Body Composition, and Suppresses Gonadal Maturation in All-Female Giant Freshwater Prawn, Macrobrachium rosenbergii

Nervous and humoral control of cardiac performance in the winter flounder(Pleuronectes americanus)

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The impact of feeding β₂‐adrenergic agonists on rainbow trout muscle β₂‐adrenoceptors and protein synthesis