While design modifications present on work boots improve safety, they may not always provide optimal human performance during work tasks. Understanding the impact of these different design features on biomechanical and physiological postural control and locomotion variables can aid in better design modifications that can provide a safe and efficient human performance. This brief review focuses on a series of studies conducted by the current research team, that have tested three different work boots (SB: high-top steel-toed work boots; TB: high-top tactical work boots; SR: low-top slip-resistant work boots). The series of studies included testing of these work boots or combinations of them under acute and chronic simulated occupational workloads, assessing biomechanical variable such as postural stability, gait, slips, and muscle activity, as well as physiological variables such as heart rate, energy expenditure, oxygen consumption, and pain perception. The impact of each of the work boots and their design feature on postural control and locomotion are summarized from these studies’ previously published literature. Finally, work boot design suggestions for optimal human performance are provided for better work boot selection, modification, and design.