The Impact of Helicobacter pylori Infection on the Gastric Microbiota of the Rhesus Macaque

Martín, Miriam; Bhatnagar, Srijak; George, Michael; Paster, Bruce J.; Canfield, Don R.; Eisen, Jonathan A.; Solnick, Jay V.

doi:10.1371/journal.pone.0076375

Cited by 48 publications

(49 citation statements)

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“…10 Using six age-and gender-matched rhesus monkeys, we observed no correlation between H. pylori infection status and phylogenetic diversity or community membership at the genus level, in either the antrum or corpus. 9 However, there was a striking effect on H. suis levels. While Helicobacter was the most abundant bacterium in post-H. pylori inoculation biopsies, individual biopsies were dominated by only one Helicobacter species, suggesting that H. pylori and H. suis are competitively exclusive (Fig.…”

Section: Does H Pylori Infection Impact the Gastric Microbiota?mentioning

confidence: 98%

“…In the rhesus monkey model, the inoculation of H. pylori did not affect the community membership or structure of the oral microbiota. 9 However, there are few if any other studies that address this question, and it remains a topic for future investigations.…”

Section: Does H Pylori Infection Alter Microbial Communities or Disementioning

confidence: 99%

“…8 To begin to probe the interactions between H. pylori and the microbial community of the stomach, we recently characterized the gastric microbiota of specific pathogen (H. pylori) free rhesus monkeys (Macaca mulatta), which serve as a robust and physiological model that closely resembles human H. pylori infection, and compared the results to the microbial communities in the mouth and the gut. 9 Deep sequencing of the bacterial 16S rDNA gene identified a community profile of 221 phylotypes that was largely distinct from that of the distal gut and mouth, although there were taxa in common. The gastric community also included a second Helicobacter, the commensal H. suis, which is occasionally seen as a zoonotic infection in humans.…”

Section: Introductionmentioning

confidence: 99%

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The gastric microbial community,Helicobacter pyloricolonization, and disease

Martín

Solnick

2014

Gut Microbes

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Section: Does H Pylori Infection Impact the Gastric Microbiota?mentioning

confidence: 98%

Section: Does H Pylori Infection Alter Microbial Communities or Disementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The gastric microbial community,Helicobacter pyloricolonization, and disease

Martín

Solnick

2014

Gut Microbes

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“…Recently, molecular-based techniques were introduced, which offer us a more detailed insight into the complex microbial communities molecularly [6]. Quantitative real time PCR using 16S ribosomal RNA gene (16s rRNA) primers is useful for the determination of the bacterial composition of the microbiota [7,8].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Helicobacter pylori Eradication on the Gastrointestinal Microbiota in Patients with Duodenal Ulcer

Lin¹,

Zhou²,

Xiao³

et al. 2016

JGLD

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Background: Recent reports have indicated that Helicobacter pylori (H. pylori) might have an effect on gastrointestinal flora; moreover, gastric commensual bacteria have been observed in the development of duodenal ulcer (DU). Aims: In our study, we aimed to evaluate the effect of H. pylori eradication on gastrointestinal flora in DU patients. Methods: A case-control study was performed at Jiangsu Shengze Hospital between December, 2013 and April, 2014. The patients received antibiotic eradication therapy if H. pylori testing was positive. At least four weeks after cessation of the eradication therapy, a repeat gastroscopy was performed to collect biopsies again in the same position. Gastric mucosa samples and feces specimens were collected to extract bacteria DNA and then to quantify by real-time polymerase chain reaction (PCR). Results: After the eradication of H. pylori, an increase of Lactobacillus group, Clostridium leptum subgroup, Enterobacteria and a decrease of Clostridium coccoides subgroup were found in the antrum. In the corpus, the number of bacteria in the Lactobacillus group was increased and the expression of Clostridium coccoides subgroup was significantly down-regulated. In the feces samples, only the number in the Lactobacillus group was increased. Moreover, the distribution was significantly different between female and the male patients. Conclusions: The presence of H. pylori in the stomach suppressed the colonization with Lactobacillus group, Clostridium leptum subgroup and Enterobacteria. Gender might affect the distribution and/or recolonization of the bacteria in DU patients. Abbreviations: DU: duodenal ulcer; GI: gastrointestinal; GIN: gastrointestinal neoplasia; GU: gastric ulcer; PPI: proton pump inhibitors; UBT: urea breath test.

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“…“ H. heilmanni ” type 1 has been further classified as H. suis [8,9] . H. suis has been characterized in a the stomachs of a several nonhuman primates, including mandrill monkeys ( Mandrillus sphinx) , crab-eating ( Macaca fascicularis), and rhesus macaques ( Macaca mulatta) [6,10]. …”

mentioning

confidence: 99%

Helicobacter Species Identified in Captive Sooty Mangabeys (Cercocebus atys) with Metastatic Gastric Adenocarcinoma

et al. 2015

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Background Of all human cancers, gastric carcinoma is the one of the leading causes of death. Helicobacter pylori is considered a major etiologic agent of this disease. Spontaneously occurring gastric carcinoma is a rare diagnosis in nonhuman primates. A 2011 case report documented a high incidence of gastric adenocarcinoma in a closed colony of captive sooty mangabeys (Cercebus atys). However, H. pylori infection was not detected in these animals. Materials and Methods In this study, using archived formalin-fixed, paraffin-embedded stomach sections of these animals alternative methodologies were used to identify H. pylori and other non-H. pylori Helicobacter species. In addition, two additional cases of sooty mangabeys with metastatic gastric carcinoma are characterized. Results Using fluorescent in situ hybridization, we identified gastric H. suis in 75% of archived and new gastric carcinoma cases. In the two newly reported cases, H. suis and a novel Helicobacter species were detected via PCR and sequence analysis of the 16S rRNA gene. H. pylori was not identified in any of the gastric carcinoma cases via FISH and/or PCR and sequence analysis of Helicobacter spp. in DNA from of available tissues. Conclusions This report is the first to characterize Helicobacter species infection in spontaneous gastric carcinoma with metastatic potential in nonhuman primates.

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The Impact of Helicobacter pylori Infection on the Gastric Microbiota of the Rhesus Macaque

Cited by 48 publications

References 50 publications

The gastric microbial community,Helicobacter pyloricolonization, and disease

The gastric microbial community,Helicobacter pyloricolonization, and disease

The Effect of Helicobacter pylori Eradication on the Gastrointestinal Microbiota in Patients with Duodenal Ulcer

Helicobacter Species Identified in Captive Sooty Mangabeys (Cercocebus atys) with Metastatic Gastric Adenocarcinoma

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