The impact of impurities in various crude A. annua extracts on the analysis of artemisinin by liquid chromatographic methods

Pilkington, Joshua L.; Preston, Chris; Gomes, Rachel L.

doi:10.1016/j.jpba.2012.06.015

Cited by 9 publications

(3 citation statements)

References 16 publications

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“…Using this specific mAb, we developed an icELISA, which allows accurate quantification of artemisinin with a working range of 0.6–11.5 ng mL –1 . The limits of detection of HPLC-UV and HPLC-MS were approximately 5 μg/mL ,, and 50 ng mL –1 , , respectively, which are almost 10,000- and 100-fold lower than the immunoassay described here. The high sensitivity of this immunoassay can afford up to 1,000- to 2,000-fold dilutions of the A. annua samples to completely eliminate matrix interference, thus increasing the assay accuracy.…”

Section: Discussionmentioning

confidence: 57%

Development of a Specific Monoclonal Antibody for the Quantification of Artemisinin in Artemisia annua and Rat Serum

et al. 2016

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Artemisinin, extracted from Artemisia annua, and its derivatives are important frontline antimalarials. To produce specific antibodies for the detection and quantification of artemisinin, artemisinin was transformed to 9-hydroxyartemisinin by microbial fermentation, which was used to prepare a 9-succinate artemisinin hapten for conjugation with ovalbumin. A monoclonal antibody (mAb), designated as 3H7A10, was selected from hybridoma cell lines which showed high specificity to artemisinin. No competitive inhibition was observed with artesunate, dihydroartemisinin, and artemether for up to 20,000 ng mL−1. An indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed, which showed a concentration causing 50% of inhibition (IC50) for artemisinin as 2.6 ng mL−1 and a working range of 0.6–11.5 ng mL−1. The icELISA was applied for the quantification of artemisinin in crude extracts of wild A. annua and the study of pharmacokinetics of artemisinin in rat serum after intraperitoneal injection. The results were highly correlated with those determined by HPLC-UV analysis (R2=0.9919). In comparison with reported anti-artemisinin mAbs which have broad cross reactivity with other artemisinin derivatives, the high specificity of 3H7A10 for artemisinin will enable development of methods for quantification of artemisinin in Artemisia plants and antimalarial drugs such as Arco®, and for pharmacokinetic studies.

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Section: Discussionmentioning

confidence: 57%

Development of a Specific Monoclonal Antibody for the Quantification of Artemisinin in Artemisia annua and Rat Serum

et al. 2016

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“…It is widely applied for many purposes of analysis, including quality control of natural products, such as impurity tests and determining the active compounds. A study by Pilkington et al (2012) reported that impurities have a critical impact on the quantification of artemisinin, which may also be the case for any natural extracts and isolates [11]. Furthermore, the purity assay of α-mangostin isolate using HPLC, therapeutic effect, and cytotoxicity was evaluated [12].…”

Section: Introductionmentioning

confidence: 99%

“…An advanced application of analytical technique using UPLC-ESI/MS for quantification of α-mangostin in dietary supplements has been reported [13]. Among the employed chromatography techniques for the active compound assay, including α-mangostin [8,11,12,14], only a few studies reported the application in the isolates [12]. Especially, the previous reported studies were not specific purpose of finding standard reference candidates by measuring the purity of the intended isolates.…”

Section: Introductionmentioning

confidence: 99%

RP-HPLC Method Validation for Purity Assay of α-Mangostin Isolate

Ratih,

Budipramana,

Firmansyah

2023

MPI

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The quality of natural products regarding the purity of their active compounds, such as α-mangostin isolate from mangosteen pericarp, may vary depending on cultivation, harvest season, and isolation process. Thus, extensive isolation procedures are involved in obtaining a certain level of purity of the active compounds. Studies on the yield of α-mangostin isolate and its effectiveness as an active compound in health care have been reported. The quality parameter of the isolate as the intended active compound can be indicated by its purity level. Measuring the purity of the active compound is proposed to define the grade α-mangostin isolate as a starting material or even reference standard. The higher the purity level of α-mangostin isolate, the greater its potential as a reference standard candidate. Therefore, a selective analytical method is required to measure the purity level accurately. For this reason, a rapid analytical method to ensure α-mangostin isolate was developed and validated to confirm its purity. Separation condition semployed an X-Terra® C18 column 5 μm, 4.6 x 150 mm under an isocratic system with a mobile phase composition of MeCN:water (85:15) at a flowrate of 0.5 mL/ min and a detector wavelength of 243 nm were selected. Acceptable validation parameters of linearity in the range of 2.6 –52 μg/mL with r2 = 0,9994, Vx0 = 2.64%; accuracy 96.38 – 100.99%; precision 1.36%; and LOD/LOQ = 4.6 μg/mL/ 13.7μg/mL were achieved. The validated method was successfully applied to the purity assay α-mangostinisolate with a run time of less than 9 minutes.

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Green Extraction of Artemisinin from Artemisia annua L

Lapkin

2015

Green Extraction of Natural Products

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The impact of impurities in various crude A. annua extracts on the analysis of artemisinin by liquid chromatographic methods

Cited by 9 publications

References 16 publications

Development of a Specific Monoclonal Antibody for the Quantification of Artemisinin in Artemisia annua and Rat Serum

Development of a Specific Monoclonal Antibody for the Quantification of Artemisinin in Artemisia annua and Rat Serum

RP-HPLC Method Validation for Purity Assay of α-Mangostin Isolate

Green Extraction of Artemisinin from Artemisia annua L

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