The impact of predatory bacteria on experimental periodontitis

Silva, Pedro Henrique Félix; Oliveira, Luana Farias de; Cardoso, Renata Silva; Ricoldi, Milla Sprone Tavares; Figueiredo, Luciene Cristina; Salvador, Sérgio Luiz de Souza; Palioto, Daniela Bazan; Furlaneto, Flávia Aparecida Chaves; Messora, Michel Reis

doi:10.1002/jper.18-0485

Cited by 19 publications

(29 citation statements)

References 47 publications

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“…Although BALOs were first isolated from soil, they are ubiquitous in nature and can be found in aquatic and terrestrial environments, including hypersaline systems 16 , biofilms 17 , mammalian intestines 18 – 20 , and, as we have shown, the lungs of cystic fibrosis (CF) patients 21 . In addition to the genetic detection of BALOs, several authors have documented the in vivo phenomenon of predation in human microbial ecosystems 22 – 25 .…”

Section: Introductionmentioning

confidence: 99%

Strain-specific predation of Bdellovibrio bacteriovorus on Pseudomonas aeruginosa with a higher range for cystic fibrosis than for bacteremia isolates

Saralegui

Herencias

Halperin

et al. 2022

Sci Rep

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This work aimed to evaluate the predatory activity of Bdellovibrio bacteriovorus 109J on clinical isolates of Pseudomonas aeruginosa selected from well-characterized collections of cystic fibrosis (CF) lung colonization (n = 30) and bloodstream infections (BSI) (n = 48) including strains selected by genetic lineage (frequent and rare sequence types), antibiotic resistance phenotype (susceptible and multidrug-resistant isolates), and colony phenotype (mucoid and non-mucoid isolates). The intraspecies predation range (I-PR) was defined as the proportion of susceptible strains within the entire collection. In contrast, the predation efficiency (PE) is the ratio of viable prey cells remaining after predation compared to the initial inoculum. I-PR was significantly higher for CF (67%) than for BSI P. aeruginosa isolates (35%) probably related to an environmental origin of CF strains whereas invasive strains are more adapted to humans. I-PR correlation with bacterial features such as mucoid morphotype, genetic background, or antibiotic susceptibility profile was not detected. To test the possibility of increasing I-PR of BSI isolates, a polyhydroxyalkanoate depolymerase deficient B. bacteriovorus bd2637 mutant was used. Global median I-PR and PE values remained constant for both predators, but 31.2% of 109J-resistant isolates were susceptible to the mutant, and 22.9% of 109J-susceptible isolates showed resistance to predation by the mutant, pointing to a predator–prey specificity process. The potential use of predators in the clinical setting should be based on the determination of the I-PR for each species, and the PE of each particular target strain.

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Section: Introductionmentioning

confidence: 99%

Strain-specific predation of Bdellovibrio bacteriovorus on Pseudomonas aeruginosa with a higher range for cystic fibrosis than for bacteremia isolates

Saralegui

Herencias

Halperin

et al. 2022

Sci Rep

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“…The use of B. bacteriovorus in the contest of periodontitis-related infection was further investigated by inducing experimental periodontitis in rats. In such conditions, it was observed that the predator promoted a protective effect against bone loss (Silva et al 2019).…”

Section: Other Applications Of Bdellovibriomentioning

confidence: 99%

Bdellovibrio bacteriovorus: a potential ‘living antibiotic’ to control bacterial pathogens

Cavallo

Jordana

Glasner

et al. 2021

Critical Reviews in Microbiology

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Bdellovibrio bacteriovorus is a small Deltaproteobacterium which, since its discovery, has distinguished itself for the unique ability to prey on other Gram-negative bacteria. The studies on this particular "predatory bacterium", have gained momentum in response to the rising problem of antibiotic resistance, because it could be applied as a potential probiotic and antibiotic agent. Hereby, we present recent advances in the study of B. bacteriovorus, comprehending fundamental aspects of its biology, obligatory intracellular life cycle, predation resistance, and potential applications. Furthermore, we discuss studies that pave the road towards the use of B. bacteriovorus as a "living antibiotic" in human therapy, focussing on its interaction with biofilms, the host immune response, predation susceptibility and in vivo application models. The available data imply that it will be possible to upgrade this predator bacterium from a predominantly academic interest to an instrument that could confront antibiotic resistant infections.

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“…Although BALOs were first isolated from soil, they are ubiquitous in nature and can be found in aquatic and terrestrial environments, including hypersaline systems (20), biofilms (21), mammalian intestines (22)(23)(24), and the lungs of patients with cystic fibrosis (25). In addition to the genetic detection of BALOs, several authors have documented the in vivo phenomenon of predation in human microbial ecosystems (26)(27)(28)(29).…”

Section: Introductionmentioning

confidence: 99%

Predation Efficiency upon Clinical Isolates: Bdellovibrio bacteriovorus is Prey Specific and Origin Dependent

Herencias

Saralegui

Halperin

et al. 2021

Preprint

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The use of predatory bacteria as live antibiotics has been proposed for managing bacterial infections, especially for those caused by antibiotic multiresistant isolates for which there are few therapeutic options. However, the current knowledge in this field is scarce, with most of the available data based on environmental isolates, with a significant lack of human clinical samples. Here, we evaluated the predatory spectrum of the reference strain Bdellovibrio bacteriovorus 109J on 13 Serratia marcescens (including carbapenemase producers) and 78 Pseudomonas aeruginosa clinical isolates from respiratory (colonizing lungs of cystic fibrosis patients) or bacteremic infections, differentiated by phenotype (mucoid or not), antibiotic resistance phenotype (including multidrug-resistant isolates), and genetic lineage (frequent and rare sequence types). The source of the isolates was significantly associated with predation efficiency (100% for S. marcescens, 67% for P. aeruginosa from cystic fibrosis, and 25% for P. aeruginosa from bacteremia). In contrast, no correlation with colonial morphotype, genetic background, or antibiotic susceptibility was found. To evaluate the influence of the predator on the predation, we employed a more aggressive B. bacteriovorus mutant 109J preying upon the same 48 bacteremic P. aeruginosa isolates. The mutant's predation efficiency was higher than that of their wild-type counterpart (43% vs. 25%), pointing out that predation is specific to each prey-predator pair. Our results provide the most extensive study of clinical prey susceptibility published to date and show that the prey-predator interaction is influenced by the origin of the isolates rather than by their genetic background or their antibiotic susceptibility phenotype.

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The impact of predatory bacteria on experimental periodontitis

Cited by 19 publications

References 47 publications

Strain-specific predation of Bdellovibrio bacteriovorus on Pseudomonas aeruginosa with a higher range for cystic fibrosis than for bacteremia isolates

Strain-specific predation of Bdellovibrio bacteriovorus on Pseudomonas aeruginosa with a higher range for cystic fibrosis than for bacteremia isolates

Bdellovibrio bacteriovorus: a potential ‘living antibiotic’ to control bacterial pathogens

Predation Efficiency upon Clinical Isolates: Bdellovibrio bacteriovorus is Prey Specific and Origin Dependent

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