The Impact of Short-Wave UV Radiation on Peroxidase Activity in Soft Wheat Seeds

Abstract

The effect of UV radiation on plants provokes oxidative stress, accompanied by the production of reactive oxygen species that can have a direct destructive effect on cellular structures and initiate free radical oxidation of proteins, lipids, and nucleic acids. There is an antioxidant defense system in plant cells that includes a complex of low and high molecular weight compounds. Antioxidants with high molecular weight are enzymes, the key of which is peroxidase [PO]. The paper studies changes in PO activity in soft spring wheat seedlings due to grain treatment with small and medium doses of short-wave UV radiation. A UV-dosed irradiation system (Bio-Link Vilber) with the exposure time 0–60 minutes in increments of 10 minutes was used for seed treatment. Pre-soaked wheat seeds were germinated in Petri dishes. PO activity was determined colorimetrically in three-day-old wheat seedlings, according to A. L. Boyarkin [3]. When the source was irradiated for up to 30 minutes, a clear dependence of the PO activity on the exposure time was not established. The maximum increase in peroxidase activity (by 25.9% relative to the control) was detected with 30-minute UV irradiation. A further increase in exposure time led to a significant decrease in enzyme activity. Moreover, vital feedback was revealed between the activity and exposure time in the range of 30–60 minutes.

Influence of germicidal ultraviolet radiation UV-C on the quality of Apiaceae spices seeds

“…Meanwhile, 45 min of exposure caused a significant decrease in POD compared to the control. These results are consistent with the findings of Sharlaeva and Chirkova [30], who observed that POD activity increased in spring wheat seeds with increasing exposure durations, peaked at 30 min by 25.9%, and that a further increase in the time after 40 min resulted in a significant decline in POD compared to control.…”

“…UV exposure has been known to stimulate the generation of reactive oxygen species (ROS), known as oxidative stress. Furthermore, at low levels of exposure, UV abiotic stressors promote the synthesis of secondary metabolites, such as carotenoids, phenolics, and flavonoids, as well as the development of antioxidant enzymes that protect plants from oxidative damage, such as membrane lipid peroxidation [27,30]. These results are comparable to those of Nasibi and M-Kalantari [27], they found that Brassica napus seedlings adapted to UV-B and UV-C as abiotic stressors by producing significantly more flavonoids and anthocyanins than the control.…”

“…Moreover, UV-B radiation strengthened the antioxidant system in barley seedlings by increasing the activity of antioxidant enzymes and causing the formation of phenolic acids [28]. The plant's antioxidant defense mechanism is influenced by the type of stressor, as well as the severity and duration of its action [30]. To the best of our knowledge, the effectiveness of decontaminated short-wave UV radiation (UV-C) on the quality of spices seeds for consumption has not been adequately discussed.…”

Novel Nanobiocomposites Based on Natural Polysaccharides as Universal Trophic Low-Dose Micronutrients

“…Therefore, changes in the activity of this enzyme can often be used to assess the body’s resistance to stress [ 45 , 46 ]. The activity of guaiacol-dependent peroxidase was determined by the Boyarkin method at the end of the plants’ co-incubation with NCs [ 47 ] ( Figure 9 ).…”

“…The concentration of MDA was determined by the method using 20% trichloroacetic acid and 0.5% thiobarbituric acid solution [ 90 ]. Peroxidase activity in potato tissues was determined according to the Boyarkin method [ 47 ].…”