The Impact of the Functional Layer Composition of Glucose Test-Strips on the Stability of Electrochemical Response

Abstract

Herein, the impact of the chemical stability of RedOx mediator ferricyanide, K3[Fe(CN)6] (FC), a type of buffer solution used for bioreceptor preparation, gel composition (carboxymethylcellulose, CMC, Aerosile, AS, and alginate, ALG) on the long term stability of glucose test-strips and their analytical performance was examined. By simple addition of ALG to the functional gel aiming to improve its viscosity, we managed to enhance the sensitivity of conventional CMC-containing amperometric glucose test-strips from 3.3 µA/mM to 3.9 µA/mM and extend their shelf life from 8 months to 1.7 years. Moreover, during the course of investigations, it was revealed that the activity of enzyme in dependence with the used buffer did not linearly correlate with its activity in a dried functional layer, and the entire long-term electrochemical signal of glucose test-strips was determined by RedOx mediator FC chemical stability. The most stable and sensitive test-strips were obtained by the screen-printing approach from a gel containing 24 mg/mL GOx prepared in citrate buffer with pH 6, 200 mg/mL of FC and 10 mg/mL of CMC supplemented with 25 mg/mL of ALG.

Electrochemistry of Freely Diffusing Mediators in Polyelectrolyte Membranes Used for Blood Glucose Test Strips with a High Upper Limit of the Linear Range

“…The enzyme immobilization techniques, as well as matrices, retaining enzyme activity are also a constant issue in research [7][8][9]. Since it seems promising to immobilize both the mediator and the enzyme in one polymer matrix, the electrochemical behavior of the mediator in this matrix is an important factor.…”