The Impact of Transportation on Physiological and Behavioral Parameters in Wistar Rats: Implications for Acclimatization Periods

Arts, Johanna W. M.; Krämer, K.; Arndt, Saskia S.; Ohl, Frauke

doi:10.1093/ilar.53.1.82

Cited by 37 publications

(31 citation statements)

References 67 publications

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“…Whereas the C57Bl/6J mice from experiment 2 were bred in the in‐house animal facility (original breeding pairs from Charles River, Germany), C57Bl/6J mice from experiment 1 were directly obtained from Charles River Spain. Apart from the different living/raising conditions of the animals, this also implicates that the mice of experiment 1 experienced a transportation process, which may have caused additional stress and thereby altered findings . Indeed, the life history of animals can influence fear responses later in life and adverse experiences, such as transport stress, can increase the responsiveness to a harmless stimulus, such as the prospective tone CS in the acclimation session of our experiments.…”

Section: Discussionmentioning

confidence: 93%

“…Apart from the different living/raising conditions of the animals, this also implicates that the mice of experiment 1 experienced a transportation process, which may have caused additional stress and thereby altered findings. 26 Indeed, the life history of animals can influence fear responses later in life and adverse experiences, such as transport stress, can increase the responsiveness to a harmless stimulus, 27 such as the prospective tone CS in the acclimation session of our experiments. Sensitization by transport stress has previously been shown in BALB/c mice.…”

Section: Discussionmentioning

confidence: 97%

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Neuropeptide‐S‐receptor deficiency affects sex‐specific modulation of safety learning by pre‐exposure to electric stimuli

Kreutzmann

Khalil

Köhler

et al. 2020

Genes Brain and Behavior

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Neuropeptide S (NPS) is a neuropeptide involved in the regulation of fear. Because safety learning is impaired in patients suffering from anxiety‐related psychiatric disorders, and polymorphisms of the human neuropeptide S receptor (NPSR) gene have also been associated with anxiety disorders, we wanted to investigate whether NPSR‐deficiency interferes with safety learning, and how prior stress would affect this type of learning. We first investigated the effect of pre‐exposure to two different types of stressors (electric stimuli or immobilization) on safety learning in female and male C57Bl/6 mice, and found that while stress induced by electric stimuli enhanced safety learning in males, there were no differences in safety learning following immobilization stress. To further investigate the role of the NPS system in stress‐induced modulation of safety learning, we exposed NPSR‐deficient mice to stress induced by electric stimuli 10 days before safety learning. In nonstressed male mice, NPSR‐deficiency enhanced safety learning. As in male C57Bl/6 mice, pre‐exposure to electric stimuli increased safety learning in male NPSR +/+ mice. This pre‐exposure effect was blocked in NPSR‐deficient male mice showing impaired, but still intact, safety learning in comparison to their NPSR +/+ and NPSR +/− littermates. There was neither a pre‐exposure nor a genotype effect in female mice. Our findings provide evidence that pre‐exposure to stress induced by electric stimuli enhances safety learning in male mice, and that NPSR‐deficiency prevents the beneficial effect of stress exposure on safety learning. We propose an inverted U‐shape relationship between stress and safety learning.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 97%

Neuropeptide‐S‐receptor deficiency affects sex‐specific modulation of safety learning by pre‐exposure to electric stimuli

Kreutzmann

Khalil

Köhler

et al. 2020

Genes Brain and Behavior

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“…For examination of the behavioral profile after being housed in the IVC or OCs, the mice in our study were subjected to a comprehensive test-battery, including anxiety-like behavior in the light-dark box, exploratory activity in open field, testing of social approach to unfamiliar conspecific, marble burrowing and nest building as speciesspecific behaviors, SIH and forced swimming test. Although we did not find any systematic study where the behavior and welfare of the mice after transportation at different ages has been investigated, there is evidence that rats display longlasting changes in physiological and behavioral parameters after transportation51 and these effects can be sex-dependent 50. Usually, the animals at commercial breeding centers are born in OCs (personal communication), and after delivery to the research institution may experience different duration of housing in new environment.…”

mentioning

confidence: 96%

Housing mice in the individually ventilated or open cages—Does it matter for behavioral phenotype?

Åhlgren

Võikar

2019

Genes Brain and Behavior

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Individually ventilated caging (IVC) systems for rodents are increasingly common in laboratory animal facilities. However, the impact of such substantial change in housing conditions on animal physiology and behavior is still debated. Most importantly, there arise the questions regarding reproducibility and comparison of previous or new phenotypes between the IVC and open cages. The present study was set up for detailed and systematic comparison of behavioral phenotypes in male and female mice of three widely used inbred strains (C57BL/6JRccHsd, DBA/2JRccHsd, 129S2/SvHSd) after being kept in two housing environments (IVC and open cages) for 6 weeks (since 4 weeks of age) before behavioral testing. The tests addressed exploratory, anxiety‐like and stress‐related behavior (light‐dark box, open field, forced swim test, stress‐induced hyperthermia), social approach and species‐specific behavior (nest building, marble burying). In all tests, large and expected strain differences were found. Somewhat surprisingly, the most striking effect of environment was found for basal body temperature and weight loss after one night of single housing in respective cages. In addition, the performance in light‐dark box and open field was affected by environment. Several parameters in different tests showed significant interaction between housing and genetic background. In summary, the IVC housing did not invalidate the well‐known differences between the mouse strains which have been established by previous studies. However, within the strains the results can be influenced by sex and housing system depending on the behavioral tasks applied. The bottom‐line is that the environmental conditions should be described explicitly in all publications.

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“…The animals need adaptation process with the new environment which may be different from the previous one. The adaptation should be carried out at least in a few days after their arrival because on the way to the new place, the animals experience stress and distress (Obernier and Baldwin 2006); consequently, this influences the (physio biology) condition and the behavior of the animals (Arts et al 2012). The condition and environment of the new treatment is supposed to be not too different from the previous condition.…”

Section: Adaptation and Clinical Health Synchronizationmentioning

confidence: 99%

Animal Study and Pre-clinical Trials of Biomaterials

Noviana

Estuningsih

Ulum

2016

Advanced Structured Materials

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An in vivo study is one of the most important steps in the process of translating biomaterials to clinical applications. It is mostly conducted to confirm in vitro results before going further to clinical trials. Appropriate use of animal models in the in vivo studies of biomaterials and medical devices is mandatory and should meet the approved regulations and ethics as defined by both local and international regulatory bodies. These studies involve the use of various approaches and protocols in order to know the body responses both local and systemic and to find out the short-and long-term responses of the body toward the implanted biomaterials or devices. This chapter describes complete procedures and practices of in vivo studies starting from selection of appropriate animal model, pre-implantation, surgical procedure and post-implantation, and monitoring of material-host responses. Some experiences of in vivo studies done by Indonesian researchers and the development of new implants are also presented.

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The Impact of Transportation on Physiological and Behavioral Parameters in Wistar Rats: Implications for Acclimatization Periods

Cited by 37 publications

References 67 publications

Neuropeptide‐S‐receptor deficiency affects sex‐specific modulation of safety learning by pre‐exposure to electric stimuli

Neuropeptide‐S‐receptor deficiency affects sex‐specific modulation of safety learning by pre‐exposure to electric stimuli

Housing mice in the individually ventilated or open cages—Does it matter for behavioral phenotype?

Animal Study and Pre-clinical Trials of Biomaterials

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