In the context of urbanization, research on urban microclimate and thermal comfort has become one of the themes of eco-city design. Sky view factor (SVF), one of the parameters of urban spatial form, combines multiple morphological information, such as plane opening, aspect ratio, and building density and has an important impact on the urban microclimate. However, there is still no clear research conclusion on the correlation between SVF and microclimate. In this paper, nine Local Climate Zone (LCZ) models are used and typical summer meteorological conditions of Nanjing are applied as an attempt to partially fill this gap. The calculated microclimate and thermal comfort indices include air temperature (AT), surface temperature (ST), relative humidity (RH), wind speed (WS), mean radiant temperature (MRT), and predicted mean vote (PMV). Results show that the local effect of urban morphology on thermal comfort can be retrieved from the use of comprehensive parameters such as SVF (which takes into account the building height, layout, and density) whose distribution in the investigated models showed to be correlated with MRT, so did PMV under low wind speed conditions.