The impact on nitrogen-efficient phenotypes when aspartate aminotransferase is expressed tissue-specifically in Brassica napus

McAllister, Chandra H.; Wolansky, Mark; Good, Allen G.

doi:10.1016/j.neps.2016.03.001

Cited by 10 publications

(11 citation statements)

References 56 publications

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“…Asparaginase hydrolyses asparagine into aspartic acid and an ammonia molecule, which is recycled by the GS/GOGAT cycle into glutamate or by aspartate aminotransferase into the glutamate, further elevating the glutamate content 41 . Consistent with this www.nature.com/scientificreports www.nature.com/scientificreports/ study, a similar increase in the concentration of aspartic acid in seeds via the overexpression of the AAT gene was reported 24,25 . Because every transgenic line is an independent event and the position of gene insertion in a tetraploid genome affects the translation and thus end-product accumulation differently, therefore, the aspartic acid concentration was weakly correlated (+0.43) with the gene expression data.…”

Section: Phenotypic Analysis Of Transgenic Cotton Lines Though the Esupporting

confidence: 84%

“…Interestingly, under the same conditions, all transgenic cotton lines showed faster growth, increased height, number of bolls, and lint yield as compared to control group (which showed stunted growth). This clearly demonstrated the behavior of transgene on the growth of cotton plants while contradicting the results of 24,25 . An intricate balance of N supply and demand is a pre-requisite for healthy plant growth 41 .…”

Section: Phenotypic Analysis Of Transgenic Cotton Lines Though the Ementioning

confidence: 69%

“…Previous researches were addressed to improve the nutritional deficiencies of plant by manipulating aspartic acid synthesis pathway 24,25 and in decreasing the asparagine concentration, which is acrylamide precursor-a cancer-causing agent 14 . To the best of our knowledge, no one has considered the role of aspartic acid/glutamic acid against the feeding-insect related plant defense systems.…”

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confidence: 99%

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Constitutive expression of Asparaginase in Gossypium hirsutum triggers insecticidal activity against Bemisia tabaci

Gul¹,

Hussain²,

Iqbal³

et al. 2020

Sci Rep

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Whitefly infestation of cotton crop imparts enormous damage to cotton yield by severely affecting plant health, vigour and transmitting Cotton Leaf Curl Virus (CLCuV). Genetic modification of cotton helps to overcome both the direct whitefly infestation as well as CLCuV based cotton yield losses. We have constitutively overexpressed asparaginase (ZmASN) gene in Gossypium hirsutum to overcome the cotton yield losses imparted by whitefly infestation. We achieved 2.54% transformation efficiency in CIM-482 by Agrobacterium-mediated shoot apex transformation method. The relative qRT-PCR revealed 40-fold higher transcripts of asparaginase in transgenic cotton line vs. non-transgenic cotton lines. Metabolic analysis showed higher contents of aspartic acid and glutamic acid in seeds and phloem sap of the transgenic cotton lines. Phenotypically, the transgenic cotton lines showed vigorous growth and height, greater number of bolls, and yield. Among six representative transgenic cotton lines, line 14 had higher photosynthetic rate, stomatal conductance, smooth fiber surface, increased fiber convolutions (SEM analysis) and 95% whitefly mortality as compared to non-transgenic cotton line. The gene integration analysis by fluorescence in situ hybridization showed single copy gene integration at chromosome number 1. Collectively, asparaginase gene demonstrated potential to control whitefly infestation, post-infestation damages and improve cotton plant health and yield: a prerequisite for farmer's community. L-asparaginases play an important role in bacteria, fungi, plants, and the tissues of some animals excluding humans 1. In plants, asparaginases are key players in regulating asparagine (primary N source) by releasing ammonia and aspartate via the deamidation pathway, which is the preferred route for developing plant organs. The aspartic acid resulting from this pathway is incorporated into the aspartate family of amino acids 2,3. There are two reported categories of asparaginases: plant and microbial. Plant asparaginases are further subdivided into two subclasses based on their dependency on potassium (K +): K +-dependent and K +-independent. These are isolated from various plants including Arabidopsis thaliana, Lupinus albus, Lupinus polyphyllus, Phaseolus vulgaris 4 , Lupinus arboreus, Lupinus angustifolius 5 , Lotus japonicus 6 , Glycine max 7 , Withania somnifera 8,9 , and Pisum sativum 10. K +-dependent asparaginases exhibit asparaginase activity 11,12 whereas K +-independent asparaginases (isolated from Lupinus luteus (LlA)) possess both isoaspartyl peptidase and asparaginase activity and recognize β-aspartyl-His as a substrate 3,13. Under nitrogen-limiting conditions, the stored asparagine is the main source of nitrogen for the growing parts of plants, i.e., developing leaves and seeds 14. A total of 81 % of the global cotton fiber stems from genetically modified cotton; GM cotton helps combat various biotic and abiotic stresses 15 that negatively affect the quality and quantity of fiber 16. In 2017, Rahman et al. (2...

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Section: Phenotypic Analysis Of Transgenic Cotton Lines Though the Esupporting

confidence: 84%

Section: Phenotypic Analysis Of Transgenic Cotton Lines Though the Ementioning

confidence: 69%

mentioning

confidence: 99%

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Constitutive expression of Asparaginase in Gossypium hirsutum triggers insecticidal activity against Bemisia tabaci

Gul¹,

Hussain²,

Iqbal³

et al. 2020

Sci Rep

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“…The drastic reduction of GOGAT under N-stress condition in both the genotypes indicates that no further transamination in chloroplast is preferred by either of these genotypes; possibly, glutamine itself is used as a N-transport molecule, or it transfers its amido group to aspartate in the presence of cytosolic asparagine synthatase in cytosol (Lam et al 2003). In fact, alanine aminotransferase (AlaAT or ALT), asparagine synthase (AS), aspartate aminotransferase (AspAT), have all been implicated in modulating NUE (Shrawat et al 2008;McAllister et al 2016). We observed downregulation of Osh36 (Os05g0475400 encoding AAT like protein) in N22, and ALT-2 in both the genotypes but more predominantly in N22.…”

Section: Discussionmentioning

confidence: 98%

Integration of Dual Stress Transcriptomes and Major QTLs From a Pair of Genotypes Contrasting for Drought and Chronic Nitrogen Starvation Identifies Key Stress Responsive Genes in Rice

Sevanthi

Sinha

Sureshkumar

et al. 2020

Preprint

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We report here the genome-wide changes resulting from low N (N-W+), low water (N+W-) and dual stresses (N-W-) in root and shoot tissues of two rice genotypes, namely, IR 64 (IR64) and Nagina 22 (N22), and their association with the QTLs for nitrogen use efficiency. For all the root parameters, except for root length under N-W+, N22 performed better than IR64. Chlorophyll a, b and carotenoid content were higher in IR64 under N+W+ treatment and N- and W- stresses; however, under dual stress, N22 had higher chlorophyll b content. While nitrite reductase, glutamate synthase (GS) and citrate synthase assays showed better specific activity in IR64, glutamate dehydrogenase showed better specific activity in N22 under dual stress; the other N and C assimilating enzymes showed similar but low specific activities in both the genotypes. A total of 8926 differentially expressed genes (DEGs) were identified compared to optimal (N+W+) condition from across all treatments. While 1174, 698 and 903 DEGs in IR64 roots and 1197, 187 and 781 in N22 roots were identified, nearly double the number of DEGs were found in the shoot tissues; 3357, 1006 and 4005 in IR64 and 4004, 990 and 2143 in N22, under N-W+, N+W- and N-W- treatments, respectively. IR64 and N22 showed differential expression in 15 and 11 N-transporter genes respectively, under one or more stress treatments, out of which four showed differential expression also in N+W- condition. The negative regulators of N- stress, e.g., NIGT1, OsACTPK1 and OsBT were downregulated in IR64 while in N22, OsBT was not downregulated. Overall, N22 performed better under dual stress conditions owing to its better root architecture, chlorophyll and porphyrin synthesis and oxidative stress management. We identified 12 QTLs for seed and straw N content using 253 recombinant inbred lines derived from IR64 and N22 and a 5K SNP array. Three QTLs co-localized on chromosome 6 spanning 417 Kbp and comprising of 31 genes, of which, five were DEGs including two UDP-glucuronosyl/UDP-glucosyltransferase family proteins. The DEGs, QTLs and candidate genes reported in this study can serve as a major resource for both rice improvement and functional biology.

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“…Isso porque, em primeiro lugar, ambas utilizam substratos que são intermediários tanto do metabolismo do carbono quanto do nitrogênio, isto é, 2-oxoglutarato, aspartato e glutamato. Em segundo lugar, estas enzimas exercem um impacto direto nas concentrações de glutamato e 2-oxoglutarato nas células, consequentemente afetando o metabolismo de N na planta (MCALLISTER et al, 2016). Assim, a manipulação genética destes genes pode se constituir em uma opção viável para alterar o equilíbrio bioquímico entre o metabolismo do nitrogênio e do carbono, visando a obtenção de plantas com maior EUN (GOOD; BEATTY, 2011).…”

Section: Introductionunclassified

Eficiência Do Uso De Nitrogênio Em Plantas Transgênicas De Tabaco Com Alto Acúmulo De Prolina

Viudes¹,

Silva

Ribas

et al. 2018

COLLOQ AGRARIAE

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Increasing the nitrogen use efficiencyofcrop plants is a result of the improvement in the mechanisms involved in nitrogen uptake, translocation, assimilation and remobilization. In this study, tobacco plants with high accumulation of proline caused by the insertion of the VaP5CSF129Amutant gene under control of the constitutive promoter CaMV35S was used as a model to investigate the effect of overexpression of the transgene in the uptake and utilization of N. For this, an experiment was carried out in a completely randomized design, in a 2x5 factorial arrangement, with two genotypes (transgenic eventand untransformed control) and five levels of nitrogen fertilization (25, 50, 100, 200 and 400 kg N ha-¹), cultivated under greenhouse conditions. Plants were evaluated for height, number of leaves, dry mass, free proline content, nitrogencontent, total protein content and use of the applied N (efficiency of absorption -EAbs, efficiency of leaf utilization –EUtiland efficiency of plant use -EUN). The transgenic plantswith high proline accumulation were less efficient than the control plants in terms of nitrogen absorption (with lowerN levelsin root, leaves and wholeplant), which resulted in lower EAbs. There was no difference between the transgenic and control genotypesfor EUtil. However, the transgenic plants presented lower EUN in comparison tothe control plants. Our data showed thattransgenictobacco plants withhigh proline accumulation do not have potential for direct use in agricultural systems, since the alteration of the biochemical balance between the metabolisms of nitrogen and carbon did not result in plants with higher EUN.

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The impact on nitrogen-efficient phenotypes when aspartate aminotransferase is expressed tissue-specifically in Brassica napus

Cited by 10 publications

References 56 publications

Constitutive expression of Asparaginase in Gossypium hirsutum triggers insecticidal activity against Bemisia tabaci

Constitutive expression of Asparaginase in Gossypium hirsutum triggers insecticidal activity against Bemisia tabaci

Integration of Dual Stress Transcriptomes and Major QTLs From a Pair of Genotypes Contrasting for Drought and Chronic Nitrogen Starvation Identifies Key Stress Responsive Genes in Rice

Eficiência Do Uso De Nitrogênio Em Plantas Transgênicas De Tabaco Com Alto Acúmulo De Prolina

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The impact on nitrogen-efficient phenotypes when aspartate aminotransferase is expressed tissue-specifically in Brassica napus

Cited by 10 publications

References 56 publications

Constitutive expression of Asparaginase in Gossypium hirsutum triggers insecticidal activity against Bemisia tabaci

Constitutive expression of Asparaginase in Gossypium hirsutum triggers insecticidal activity against Bemisia tabaci

Integration of Dual Stress Transcriptomes and Major QTLs&nbsp;From a Pair of Genotypes Contrasting for Drought and Chronic Nitrogen Starvation Identifies Key Stress Responsive Genes in Rice

Eficiência Do Uso De Nitrogênio Em Plantas Transgênicas De Tabaco Com Alto Acúmulo De Prolina

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Integration of Dual Stress Transcriptomes and Major QTLs From a Pair of Genotypes Contrasting for Drought and Chronic Nitrogen Starvation Identifies Key Stress Responsive Genes in Rice