The impacts of As accumulation under different pH levels: Comparing Ruditapes decussatus and Ruditapes philippinarum biochemical performance

Abstract

Marine bivalves have been used to assess environmental As contamination and the effects of seawater acidification when both factors are acting alone, but limited information is available regarding the impacts of both factors acting in combination. The aim of this study was to compare physiological (glycogen) and biochemical (lipid peroxidation, superoxide dismutase, catalase, glutathione-S-transferase and alkaline phosphatase) responses in both native (Ruditapes decussatus) and introduced (R. philippinarum) clams, when exposed to the combined effects of pH (7.8, control; 7.3) and As concentrations (0 and 4mg/L). The combined effect of As and pH on the health risks associated with clam consumption was also analyzed. Results revealed that both species were able to accumulate As under both pH levels, although higher As concentrations where observed under low pH. Thus, predicted pH decrease will potentiate health risks associated with the consumption of such species, since less amount of clams exposed to As is needed for an adult to exceed the provisional tolerable weekly intake (PTWI). Low pH, As exposure and the combination of both factors did not negatively affect the native species, since clams were able to maintain their physiological and biochemical performance among all conditions. On the other hand, R. philippinarum was negatively affected by As exposure at control pH (7.8), inducing biotransformation and antioxidant defense mechanisms against As toxicity. R. philippinarum exposed and non-exposed to As presented similar responses under low pH although at this condition the introduced species accumulated twice the amount of As than R. decussatus.

Biochemical and Behavioural Alterations Induced by Arsenic and Temperature in Hediste diversicolor of Different Growth Stages

“…diversicolor usually increased when exposed to contaminants, such as mercury [ 75 ]. The same pattern was observed with Diopatra neapolitana when exposed to arsenic and to metals and As [ 22 , 24 ] and with the bivalve Ruditapes philippinarum exposed to As [ 101 ].…”

Biochemical and physiological responses of two clam species to Triclosan combined with climate change scenario

“…Although the manila clam consistently revealed higher LPO levels and the European clam presented higher levels of PC in the whole tissue, this characteristic could be a reflection of their natural biochemical composition and not a deleterious sign since oxidised by-products formation was not induced by exposure to TCS alone or combined with physical stressors. Moreover, in accordance to a higher antioxidant pool (CAT, GPx and GSTs activities) in R. decussatus, LPO levels were consistently lower in this species as formerly pointed out (Costa et al, 2020;Velez et al, 2016c). However, remarkable higher PC values were observed in the native clam at both scenarios, despite the total PROT content did not follow this species trend.…”

Toxic effects of multi-walled carbon nanotubes on bivalves: Comparison between functionalized and nonfunctionalized nanoparticles

“…peroxidation (LPO)) and neurotoxicity (e.g., acetylcholinesterase (AChE)) (Bebianno et al, 2004). While several studies showed that these biochemical markers are useful in detecting possible damages under metal pollution (Velez et al, 2016;Wang et al, 2011;Yesudhason et al, 2013;Gomes et al, 2013) eutrophication (Verdelhos et al, 2005) and pharmaceuticals (Freitas et al, 2015;Antunes et al, 2013;Matozzo et al, 2016b;Correia et al, 2016), less is known on the biochemical alterations induced by CNMs in this group of organisms (Cattaneo et al, 2009;De Marchi et al, 2017a;Canesi and Corsi, 2015;Miller et al, 2015;Matranga and Corsi, 2012;De Marchi et al, 2017b).…”