The implementation of high fermentative 2,3-butanediol production from xylose by simultaneous additions of yeast extract, Na2EDTA, and acetic acid

Wang, Xiaoxiong; Hu, Hong-Ying; Liu, Dehua; Song, Yuanquan

doi:10.1016/j.nbt.2015.07.004

Cited by 22 publications

(12 citation statements)

References 23 publications

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“…This finding suggests that K. oxytoca UM2‐17 redirected its metabolism towards biomass generation. These results contrast with those of Asenjo and Merchuk (1994), where yeast extract and peptone supplementation maintained alcohol production rate and improved 2,3‐butanediol production …”

Section: Discussionsupporting

confidence: 89%

“…These results contrast with those of Asenjo and Merchuk (1994), 38 where yeast extract and peptone supplementation maintained alcohol production rate and improved 2,3-butanediol production. [39][40][41][42] The 2,3-butanediol yield is dependent on the sugar concentration used in the fermentation medium 2 ; the suitable sugar concentration range to produce 2,3-butanediol with maximal yield was defined as 20-100 g L −1 of glucose or xylose, because exceeding 150 g L −1 causes cellular inhibition, probably due to accumulation of subproducts such as ethanol and acetate [ Fig. 1(a)].…”

Section: Discussionmentioning

confidence: 99%

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Production of 2,3‐butanediol by fermentation of enzymatic hydrolysed bagasse from agave mezcal‐waste using the native Klebsiella oxytoca UM2‐17 strain

Pasaye-Anaya

Vargas‐Tah

Martínez‐Cámara

et al. 2019

J of Chemical Tech & Biotech

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Background 2,3‐butanediol is a chemical component with wide industrial applications, such as biofuels and chemical precursors. Reducing 2,3‐butanediol production costs is a high priority and recent focus has been on its biological synthesis from raw renewable materials rather than through conventional chemical procedures. In this study, a 2,3‐butanediol native bacterial producer was selected for the fermentation of Agave cupreata bagasse waste by enzymatic hydrolysation to obtain 2,3‐butanediol. Results The native Klebsiella oxytoca UM2‐17 strain was selected to efficiently produce 2,3‐butanediol on synthetic mineral media. Using glucose (100 g L−1) and glucose: xylose (50 g L−1:50 g L−1) as sugar sources obtained titres of 29.36 g L−1 and 25.9 g L−1 of 2,3‐butanediol, corresponding to yields of 0.29 g g−1 and 0.26 g g−1, respectively. Agave bagasse solid waste after acid pre‐treatment was utilized to obtain enzymatic hydrolysates, obtaining liquors with glucose/xylose/arabinose at 25.8 g L−1 with efficiency of ∼52.5% sugar conversion from residual solid pre‐hydrolysed bagasse. The production of 2,3‐butanediol through batch fermentation of the enzymatic‐hydrolysate by the UM2‐17 strain showed total depletion of glucose and xylose, achieving a maximal production of 10.3 g L−1 of 2,3‐butanediol plus 0.28 g L−1 ethanol, corresponding to 2,3‐butanediol yield of 0.40 g g−1. Conclusion The results indicate that A. cupreata bagasse waste from mezcal beverage elaboration is a sustainable bioresource for production of 2,3‐butanediol by fermentation of enzymatic bagasse‐hydrolysate using the K. oxytoca UM2‐17 strain. © 2019 Society of Chemical Industry

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Production of 2,3‐butanediol by fermentation of enzymatic hydrolysed bagasse from agave mezcal‐waste using the native Klebsiella oxytoca UM2‐17 strain

Pasaye-Anaya

Vargas‐Tah

Martínez‐Cámara

et al. 2019

J of Chemical Tech & Biotech

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“…Furthermore, downstream processing using inorganic nitrogen in the culture medium is less complicated, which allows for more efficient purification of 2,3‐BDO 2 . Supplements with acetic acid, 62,64 sodium bicarbonate, 41 and iron 73 have also been verified. Acetate is an important inducer of the key enzymes of the 2,3‐BDO metabolism, in which its yield can be increased by adding acetic acid / acetate to the culture medium 10 .…”

Section: Resultsmentioning

confidence: 95%

“…Residual saccharin sugars, lignocellulosic materials, and the biodiesel‐derived glycerol were the most used feedstocks in bio‐based 2,3‐BDO production. The main raw materials verified were: artichoke biomass, 37,55–57 vinegar, 36 corn hydrolysate, 58,59 xylose, 6,33,60–63 whey, 31,43 oil palm, 64 cassava, 35,44 and crude glycerol 50,65 . Despite presenting a reduction in the long term, research on the substrate must still be extensively carried out due to its importance in the bioprocess economy on a large scale, as substrate costs can represent up to 50% of the production costs 11 .…”

Section: Resultsmentioning

confidence: 99%

Technological development of the bio‐based 2,3‐butanediol process

Tinôco

Borschiver

Coutinho

et al. 2020

Biofuels Bioprod Bioref

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The biotechnological production of 2,3‐butanediol (2,3‐BDO) has economic and environmental advantages over the chemical route, although its large‐scale implementation has not yet been consolidated. To establish the technological maturity of the bio‐based 2,3‐BDO process, and the factors limiting its industrial use, a technology roadmap was built from patents and scientific articles, in which four drivers were identified: production process, recovery process, product, and application. The production process driver was reported in 53.25% of documents, notably including studies on substrates, engineered microorganisms, efficient production methods, and nutritional supplementation. The recovery process driver was investigated in 23.5% of studies, with conventional distillation and solvent extraction being the main technologies performed on a large scale. Product and application drivers accounted for 9.25% and 14% of studies, respectively, highlighting the chemical intermediates, plastics, food additives, and cosmetics market segments. A limited number of companies like LanzaTech and GS Caltex Corporation already produce industrially bio‐based 2,3‐BDO, while academic centers such as the Yancheng Institute of Technology are focused to the laboratory scale with technologies to be implemented in the long term. This study can therefore help to establish and gain an understanding of the necessary strategies to consolidate the industrial production of bio‐based 2,3‐BDO in the future. © 2020 Society of Chemical Industry and John Wiley & Sons, Ltd

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“…Some bio-based monomers have been used for the synthesis of polyester-type plastics, such as butanediol, isosorbide, bicyclic dicarboxylic acid, galactose and furan-2,5-dicarboxylic acid. 2,3-butanediol is a promising and valuable chemical that can be used in various areas as a liquid fuel and a platform monomer [20], and it can be produced by conventional fermentation from various bioresources such as xylose, sugar cane molasses, corn and glucose [21]. It has pendent groups, which can effectively inhibit the crystallization of the polyester elastomer.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Properties of Novel Thermoplastic Vulcanizate Based on Bio-Based Polyester/Polylactic Acid, and Its Application in 3D Printing

Gao

et al. 2017

Polymers

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Thermoplastic vulcanizate (TPV) combines the high elasticity of elastomers and excellent processability of thermoplastics. Novel bio-based TPV based on poly (lactide) (PLA) and poly (1,4-butanediol/2,3-butanediol/succinate/itaconic acid) (PBBSI) were prepared in this research. PBBSI copolyesters were synthesized by melting polycondensation, and the molecular weights, chemical structures and compositions of the copolyesters were characterized by GPC, NMR and FTIR. Bio-based 2,3-butanediol was successfully incorporated to depress the crystallization behavior of the PBBSI copolyester. With an increase of 2,3-butanediol content, the PBBSI copolyester transformed from a rigid plastic to a soft elastomer. Furthermore, the obtained TPV has good elasticity and rheological properties, which means it can be applied as a 3D-printing material.

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The implementation of high fermentative 2,3-butanediol production from xylose by simultaneous additions of yeast extract, Na2EDTA, and acetic acid

Cited by 22 publications

References 23 publications

Production of 2,3‐butanediol by fermentation of enzymatic hydrolysed bagasse from agave mezcal‐waste using the native Klebsiella oxytoca UM2‐17 strain

Production of 2,3‐butanediol by fermentation of enzymatic hydrolysed bagasse from agave mezcal‐waste using the native Klebsiella oxytoca UM2‐17 strain

Technological development of the bio‐based 2,3‐butanediol process

Preparation and Properties of Novel Thermoplastic Vulcanizate Based on Bio-Based Polyester/Polylactic Acid, and Its Application in 3D Printing

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