The importance of antibody orientation in the electrochemical detection of ferritin

Matysiak-Brynda, Edyta; Wagner, Barbara; Bystrzejewski, M.; Grudziński, Ireneusz P.; Nowicka, Anna M.

doi:10.1016/j.bios.2018.02.063

Cited by 18 publications

(5 citation statements)

References 39 publications

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“…The ease of stably modifying magnetic nanomaterials through aryldiazonium ion grafting to core−shell NPs was exploited by Matysiak-Brynda et al 161 who deposited carboxyphenyl and aminoethylphenyl layers on Fe@C NPs physisorbed on gold electrodes. After a monoclonal antibody for ferritin was coupled to the anchor layers, both the interaction of ferritin with the sensing surface and the electrochemical detection of ferritin were examined in the presence and absence of an external magnetic field.…”

Section: Applicationsmentioning

confidence: 99%

Building Tailored Interfaces through Covalent Coupling Reactions at Layers Grafted from Aryldiazonium Salts

Fitchett

Brooksby

et al. 2021

ACS Appl. Mater. Interfaces

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Aryldiazonium ions are widely used reagents for surface modification. Attractive aspects of their use include wide substrate compatibility (ranging from plastics to carbons to metals and metal oxides), formation of stable covalent bonding to the substrate, simplicity of modification methods that are compatible with organic and aqueous solvents, and the commercial availability of many aniline precursors with a straightforward conversion to the active reagent. Importantly, the strong bonding of the modifying layer to the surface makes the method ideally suited to further on-surface (postfunctionalization) chemistry. After an initial grafting from a suitable aryldiazonium ion to give an anchor layer, a target species can be coupled to the layer, hugely expanding the range of species that can be immobilized. This strategy has been widely employed to prepare materials for numerous applications including chemical sensors, biosensors, catalysis, optoelectronics, composite materials, and energy conversion and storage. In this Review our goal is first to summarize how a target species with a particular functional group may be covalently coupled to an appropriate anchor layer. We then review applications of the resulting materials.

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Section: Applicationsmentioning

confidence: 99%

Building Tailored Interfaces through Covalent Coupling Reactions at Layers Grafted from Aryldiazonium Salts

Fitchett

Brooksby

et al. 2021

ACS Appl. Mater. Interfaces

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“…Electrochemical methods are often attractive in clinical diagnostics settings, due to their ease of operation, minimal sample preparation, and easy data interpretation [11]. Recently, ferritin detection using electrochemical techniques has gained attraction [12,13], employing static systems [12][13][14][15][16][17][18]. Researchers of this manuscript have also recently reported the use of biosurfactant functionalized tungsten disulfide quantum dots for the electrochemical detection of ferritin using screen printed electrodes as a platform [19].…”

Section: Introductionmentioning

confidence: 92%

Microfluidic-Based Electrochemical Immunosensing of Ferritin

et al. 2020

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Ferritin is a clinically important biomarker which reflects the state of iron in the body and is directly involved with anemia. Current methods available for ferritin estimation are generally not portable or they do not provide a fast response. To combat these issues, an attempt was made for lab-on-a-chip-based electrochemical detection of ferritin, developed with an integrated electrochemically active screen-printed electrode (SPE), combining nanotechnology, microfluidics, and electrochemistry. The SPE surface was modified with amine-functionalized graphene oxide to facilitate the binding of ferritin antibodies on the electrode surface. The functionalized SPE was embedded in the microfluidic flow cell with a simple magnetic clamping mechanism to allow continuous electrochemical detection of ferritin. Ferritin detection was accomplished via cyclic voltammetry with a dynamic linear range from 7.81 to 500 ng·mL−1 and an LOD of 0.413 ng·mL−1. The sensor performance was verified with spiked human serum samples. Furthermore, the sensor was validated by comparing its response with the response of the conventional ELISA method. The current method of microfluidic flow cell-based electrochemical ferritin detection demonstrated promising sensitivity and selectivity. This confirmed the plausibility of using the reported technique in point-of-care testing applications at a much faster rate than conventional techniques.

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“…The influence of antibody attachment to the phenyl films containing either -COOH or -NH 2 groups on the efficiency of antibody ferritin binding has been reported by Matysiak-Brynda and co-workers. The authors have observed that the vertical orientation of antibody vs the electrode surface could be achieved by its binding to aminoethylophenyl linkers leading to the increasing efficiency of sensor toward electrochemical detection of ferritin [26]. ∆I = I n − I, which means: I n -value of the peak current measured in the presence of particular "n" concentration of LDL, recorded at the potential of maximum peak current value registered in buffer, I-value of peak current measured in a solution free of LDL, in buffer.…”

Section: Optimization Of Immunosensor Preparationmentioning

confidence: 99%

Detection of Low Density Lipoprotein—Comparison of Electrochemical Immuno- and Aptasensor

Rudewicz-Kowalczyk

Grabowska

2021

Sensors

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An elevated level of low density lipoprotein (LDL) can lead to the cardiovascular system-related diseases, such as atherosclerosis and others. Therefore, fast, simple, and accurate methods for LDL detection are very desirable. In this work, the parameters characterizing the electrochemical immuno-and aptasensor for detection of LDL have been compared for the first time. An immunosensor has been designed, for which the anti-apolipoprotein B-100 antibody was covalently attached to 4-aminothiophenol (4-ATP) on the surface of the gold electrode. In the case of an aptasensor, the gold electrode was modified in a mixture of ssDNA aptamer specific for LDL modified with –SH group and 6-mercaptohexanol. Square-wave voltammetry has been used for detection of LDL in PBS containing redox active marker, [Fe(CN)6]3−/4−. Our results show the linear dependence of [Fe(CN)6]3−/4− redox signal changes on LDL concentration for both biosensors, in the range from 0.01 ng/mL to 1.0 ng/mL. The limit of detection was 0.31 and 0.25 ng/mL, for immuno- and aptasensor, respectively. Whereas slightly better selectivity toward human serum albumin (HSA), high density lipoprotein (HDL), and malondialdehyde modified low density lipoprotein (MDA-LDL) has been observed for aptasensor. Moreover, the other components of human blood serum samples did not influence aptasensor sensitivity.

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The importance of antibody orientation in the electrochemical detection of ferritin

Cited by 18 publications

References 39 publications

Building Tailored Interfaces through Covalent Coupling Reactions at Layers Grafted from Aryldiazonium Salts

Building Tailored Interfaces through Covalent Coupling Reactions at Layers Grafted from Aryldiazonium Salts

Microfluidic-Based Electrochemical Immunosensing of Ferritin

Detection of Low Density Lipoprotein—Comparison of Electrochemical Immuno- and Aptasensor

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