The importance of  Campylobacter coli  in human campylobacteriosis: prevalence and genetic characterization

Gürtler, M.; Alter, Thomas; Kasimir, Sandra; Fehlhaber, K.

doi:10.1017/s0950268805004164

Cited by 48 publications

(39 citation statements)

References 28 publications

(26 reference statements)

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“…Approximately 80% of reported infections are caused by Campylobacter jejuni, and 7 to 18% of cases are attributed to C. coli. Therefore, C. coli is among the five most important bacterial etiological agents of human gastroenteritis (2,3).…”

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Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides

et al. 2016

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Despite the importance of lipooligosaccharides (LOSs) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in Campylobacter coli. In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species. Furthermore, we paired C. coli genomic information and LOS chemical composition for the first time to investigate possible associations between LOS locus class sequence diversity and biochemical heterogeneity. After identifying three new LOS locus classes, only 85% of the 144 isolates tested were assigned to a class, suggesting higher genetic diversity than previously thought. This genetic diversity is at the basis of a completely unexplored LOS structural heterogeneity. Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni. 2-Amino-2-deoxy-D-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the ␤-1=-6-linked 3-diamino-2,3-dideoxy-D-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni. Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-D-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates. Therefore, regardless of the high genetic diversity of the LOS biosynthesis locus in C. coli, we identified species-specific phenotypic features of C. coli LOS that might explain differences between C. jejuni and C. coli in terms of population dynamics and host adaptation. IMPORTANCEDespite the importance of C. coli to human health and its controversial role as a causative agent of Guillain-Barré syndrome, little is known about the genetic and phenotypic diversity of C. coli LOSs. Therefore, we paired C. coli genomic information and LOS chemical composition for the first time to address this paucity of information. We identified two species-specific phenotypic features of C. coli LOS, which might contribute to elucidating the reasons behind the differences between C. jejuni and C. coli in terms of population dynamics and host adaptation. C ampylobacteriosis is the most common bacterial foodborne disease in developed countries, with over 200,000 human cases being reported annually in the European Union alone (1). The true burden of the disease in the population is likely underestimated, as many infections result in mild gastroenteritis (1). Approximately 80% of reported infections are caused by Campylobacter jejuni, and 7 to 18% of cases are attributed to C. coli. Therefore, C. coli is among the five most important bacterial etiological agents of human gastroenteritis (2, 3).As in other Gram-negative bacteria, Campylobacter species cell surface glycoconjugates, including lipooligosaccharides (LOSs), play an important role in serum and bile resistance; resistanc...

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Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides

et al. 2016

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“…Campylobacter jejuni is considered to be the species primarily responsible for disease in humans (26); however, Campylobacter coli cases are frequently under-reported, and thus its true public health impact is unknown (16,30). In 2000, C. coli was estimated to be responsible for more than 25,000 cases in the United Kingdom (30).…”

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Longitudinal Study of the Persistence of Antimicrobial-Resistant Campylobacter Strains in Distinct Swine Production Systems on Farms, at Slaughter, and in the Environment

Quintana-Hayashi

Thakur

2012

Appl Environ Microbiol

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The objectives of this study were to compare and characterize the prevalence of antimicrobial-resistant (AR) Campylobacter in conventional and antimicrobial-free (ABF) production systems on farms, at slaughter, and in the environment. Fecal and environmental samples were collected from ABF farms (pigs, 1,239; environment, 797) and conventional farms (pigs, 1,650; environment, 1,325). At slaughter, we collected samples from carcasses, including postevisceration swabs, postchill swabs, and mesenteric lymph nodes from ABF systems (postevisceration swabs, 182; postchill swabs, 199; mesenteric lymph nodes, 184) and conventional systems (postevisceration swabs, 272; postchill swabs, 271; mesenteric lymph nodes, 255) at separate processing facilities. We also sampled the processing plant environment, including truck and lairage floor swab samples (ABF, 115; conventional, 90). Overall, a total of 2,908 Campylobacter isolates, including Campylobacter coli (farm, 2,557, 99.8%; slaughter, 341, 98.3%) and Campylobacter jejuni (farm, 4, 0.2%; slaughter, 6, 1.7%), were isolated in the study. There was no significant difference in the prevalence of Campylobacter between ABF and conventionally raised pigs (farrowing, P ‫؍‬ 0.20; nursery, P ‫؍‬ 0.06; finishing, P ‫؍‬ 0.24) and the environment (P ‫؍‬ 0.37). At slaughter, Campylobacter was isolated from all of the stages, including postchill. The highest frequencies of resistance were exhibited against tetracycline (ABF, 48.2%; conventional, 88.3%). Ciprofloxacin-resistant C. coli isolates were observed in conventionally raised (17.1%) and ABF (1.2%) pigs (P ‫؍‬ 0.11). Antimicrobial use data from conventional farms indicated significant associations between oxytetracycline use and tetracycline resistance in the nursery pigs (P ‫؍‬ 0.01), between tiamulin exposure and azithromycin and erythromycin resistance in nursery (P < 0.01) and finishing (P < 0.01) pigs, and between enrofloxacin exposure and ciprofloxacin and nalidixic acid resistance in farrowing (P < 0.01) and nursery (P < 0.01) pigs. Identical antimicrobial resistance profiles were observed in the pigs and their environments on farms and at slaughter. In summary, our results highlight the persistence and dissemination of AR Campylobacter from farm to slaughter in ABF and conventionally raised pigs and their environments.

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“…Spesies C. jejuni menyebabkan campylobacteriosis pada manusia mencapai 90% sedangkan spesies C. coli berkisar 5-10% (Gillespie et al 2002). Hasil yang diperoleh peneliti Gurtler et al (2005) adalah sebanyak 18.6% campylobacteriosis pada manusia disebabkan oleh spesies C. coli. Meskipun angka kejadiannya lebih kecil namun hal ini memperlihatkan bahwa risiko campylobacteriosis pada manusia akibat infeksi C. coli perlu diperhatikan.…”

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“…Peternakan ayam dan sapi perah serta produknya merupakan sumber utama infeksi C. jejuni sedangkan perternakan babi serta produknya berperan terhadap infeksi C. coli (Gillespie et al 2002;Alter et al 2005). Hasil penelitian Gurtler et al (2005) menyatakan bahwa spesies C. coli berperan pada infeksi Campylobacter pada manusia namun terdapat perbedaan sumber infeksi. Pada penelitian ini, pada sampel karkas ayam menunjukkan bahwa karkas telah terinfeksi oleh kedua spesies tersebut yaitu C. jejuni dan C. coli.…”

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Isolation of Campylobacter from Poultry Carcasses using Conventional and Polymerase Chain Reaction Methods

Andriani¹,

Sudarwanto

Setiyaningsih

et al. 2013

jtip

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ABSTRAKCampylobacter jejuni dan Campylobacter coli merupakan dua spesies dari Campylobacter sp. yang banyak ditemukan sebagai penyebab infeksi gastroenteritis pada manusia. Karkas ayam yang terkontaminasi merupakan sumber utama infeksi campylobakteriosis pada manusia. Pada penelitian ini, Campylobacter diisolasi dari karkas ayam yang dijual di pasar tradisional dan swalayan. Di pasar tradisional, karkas ayam dijual tanpa kemasan, di ruang terbuka dan disimpan pada suhu kamar (25-30°C) sehingga memungkinkan bakteri patogen tumbuh dan berkembang. Di pasar swalayan, karkas ayam disajikan dalam keadaan terbuka atau dalam kemasan yang disimpan pada lemari pendingin (4-8°C). Sebanyak 298 sampel karkas ayam dari pasar tradisional dan supermarket di wilayah DKI Jakarta, Jawa Barat (Bogor dan Sukabumi) dan Jawa Tengah (Kudus dan Demak) dikumpulkan, diisolasi dan diidentifikasi dengan metode konvensional dan Polymerase Chain Reactions (PCR) untuk menentukan prevalensi kontaminasi C. jejuni dan C. coli. Hasil penelitian menunjukkan karkas ayam yang dijual di pasar tradisional dan swalayan telah terkontaminasi C. jejuni dan C. coli. Tingkat kontaminasi Campylobacter sp. pada karkas ayam yang dijual di pasar swalayan ditemukan sebesar 14.1% dengan metode konvensional dan 29.5% dengan metode PCR. Angka ini lebih tinggi daripada di pasar tradisional yaitu 5.7% yang secara konvensional dan 12.1% secara PCR. Penelitian ini juga menunjukkan bahwa prevalensi kontaminasi dari C. jejuni lebih tinggi daripada C. coli pada 298 sampel, yaitu secara berturut-turut 16.1% dan 3.7% dengan metode konvensional dan 23.5% dan 18.1% menggunakan PCR.

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The importance of Campylobacter coli in human campylobacteriosis: prevalence and genetic characterization

Cited by 48 publications

References 28 publications

Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides

Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides

Longitudinal Study of the Persistence of Antimicrobial-Resistant Campylobacter Strains in Distinct Swine Production Systems on Farms, at Slaughter, and in the Environment

Isolation of Campylobacter from Poultry Carcasses using Conventional and Polymerase Chain Reaction Methods

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