The importance of glutathione in oxidative status of Rhizobium leguminosarum biovar viciae under Cd exposure

Corticeiro, Sofia; Lima, Ana; Figueira, Etelvina

doi:10.1016/j.enzmictec.2005.10.053

Cited by 54 publications

(20 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, the glutathione activity in general, was found more in roots of both rhizobium inoculated and un-inoculated plants grown in nickel and zincstressed soil suggesting that the higher concentration of these metals has probably induced the oxidative stress and generation of reactive species of oxygen , leading to the synthesis of antioxidant enzymes, which might have played a pivotal role in protecting the pea plants from the oxidative stress, as also reported for other legumes (Cardoso et al 2005;Ana et al 2006;Lima et al 2006). Furthermore, the enhanced growth of inoculated legumes under metal stress could also be due to the synthesis of glutathione reductase or other detoxifying agents by rhizobia (Figueira et al 2005;Corticeiro et al 2006). These results could play an important role in developing biotechnological strategies for metal bioremediation procedures and open novel prospective for the restoration of polluted soil using legume-Rhizobium symbiosis.…”

Section: Rhizoremediation By Symbiotic Nitrogen Fixing Organismsmentioning

confidence: 98%

“…For instance, use of plant growth promoting rhizobacteria Pseudomonad and Acinetobacter have shown to enhance phytoremediation abilities of non-hyperaccumulating maize (Zea mays L.) plants by increasing their growth and biomass (Lippmann et al 1995). Also, plants growing in metal-stressed soils can protect themselves from metal toxicity by synthesizing antioxidant enzymes, which scavenges the toxicity of reactive oxygen species generated by plants (Cardoso et al 2005) and by associative bacteria (Corticeiro et al 2006) under metal stress.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Role of plant growth promoting rhizobacteria in the remediation of metal contaminated soils

et al. 2008

View full text Add to dashboard Cite

Pollution of the biosphere by the toxic metals is a global threat that has accelerated dramatically since the beginning of industrial revolution. The primary source of this pollution includes the industrial operations such as mining, smelting, metal forging, combustion of fossil fuels and sewage sludge application in agronomic practices. The metals released from these sources accumulate in soil and in turn, adversely affect the microbial population density and physico-chemical properties of soils, leading to the loss of soil fertility and yield of crops. The heavy metals in general cannot be biologically degraded to more or less toxic products and hence, persist in the environment. Conventional methods used for metal detoxification produce large quantities of toxic products and are costeffective. The advent of bioremediation technology has provided an alternative to conventional methods for remediating the metal-poisoned soils. In metal-contaminated soils, the natural role of metal-tolerant plant growth promoting rhizobacteria in maintaining soil fertility is more important than in conventional agriculture, where greater use of agrochemicals minimize their significance. Besides their role in metal detoxification/removal, rhizobacteria also promote the growth of plants by other mechanisms such as production of growth promoting substances and siderophores. Phytoremediation is another emerging lowcost in situ technology employed to remove pollutants from the contaminated soils. The efficiency of phytoremediation can be enhanced by the judicious and careful application of appropriate heavy-metal tolerant, plant growth promoting rhizobacteria including symbiotic nitrogen-fixing organisms. This review presents the results of studies on the recent developments in the utilization of plant growth promoting rhizobacteria for direct application in soils contaminated with heavy metals under a wide range of agro-ecological conditions with a view to restore contaminated soils and consequently, promote crop productivity in metal-polluted soils across the globe and their significance in phytoremediation.

show abstract

Section: Rhizoremediation By Symbiotic Nitrogen Fixing Organismsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Role of plant growth promoting rhizobacteria in the remediation of metal contaminated soils

et al. 2008

View full text Add to dashboard Cite

show abstract

“…Cadmium is one of the most deleterious heavy metals which may induce accumulation of ROS through damaging mitochondria and affecting the process of cellular respiration (Wang et al 2004), activating xanthine oxidase and heme oxygenase (Casalino et al 1997), and reducing the activities of antioxidant enzymes (Stoch and Bagchi 1995;Corticeiro et al 2006). It has been reported that Cd-induced cell toxicity may be associated with its ability to attack lipids and proteins via generating O 2 − · and ·OH (Wang et al 2004).…”

Section: Discussionmentioning

confidence: 97%

In vivo antioxidative effect of isoquercitrin on cadmium-induced oxidative damage to mouse liver and kidney

Yuan

Dong

et al. 2011

Naunyn-Schmiedeberg's Arch Pharmacol

View full text Add to dashboard Cite

Cadmium (Cd) is an environmental pollutant and has been found to pose a potential threat to human health. Isoquercitrin (IQ) is one of the most important flavonoids and has been demonstrated to exhibit potent antioxidant effects on plants and yeast cells. However, only few studies have investigated the antioxidative activities of reactive oxygen species (ROS) and the nitrite scavenging activities of IQ against Cd-induced oxidation in mouse. The present work was to investigate the ROS and nitrite-scavenging activities of IQ in vitro as well as its preventive effects against lipid peroxidation and protein oxidative damage in liver and kidney of mouse induced by Cd(²+) using spectrophotometry. Our results showed that IQ possesses scavenging abilities for superoxide anion, hydroxyl radical and nitrite. Such scavenging capacities increase with the concentration of IQ. Moreover, cadmium chloride (CdCl₂ (2.5 mg/kg body weight, i.p. CdCl₂) significantly inhibited the activities of superoxide dismutase and catalase and raised the levels of malondialdehyde, nitric oxide, protein carbonyl, and the coefficients of DNA-protein crosslinks in livers and/or kidneys of mice. IQ attenuated the Cd(²+)-induced biochemical alterations in the livers and/or kidneys of mice, indicating that the formation of ROS and nitrite is possibly reduced. Our work demonstrates that IQ possesses ROS and nitrite-scavenging capacities and plays a significant role in combating Cd(²+)-induced toxicity in animals.

show abstract

“…the subject of the present work, very often appears to be a consequence of reactions triggered by prooxidative targets such as menadione, paraquat, metal ions and others, when the levels of produced ROS increase beyond the antioxidant capacity of cells (Jamieson 1998;Corticeiro et al 2006). Since the physiological life cycle of rhizobia during the legume-rhizobia symbiosis is strictly correlated with the relatively high concentration of reactive oxygen intermediates such as SOR, hydroxyl radicals, and hydrogen peroxide, it is evidenced that these organisms must evolve efficient anti-stress mechanisms (Hérouart et al 2002;Saenkham et al 2007).…”

Section: Discussionmentioning

confidence: 99%

The response of the Rhizobium leguminosarum bv. trifolii wild-type and exopolysaccharide-deficient mutants to oxidative stress

et al. 2013

View full text Add to dashboard Cite

Aims The aim of this study was investigation of the response of R. leguminosarum bv. trifolii wild-type and its two rosR and pssA mutant strains impaired in exopolysaccharide (EPS) synthesis to oxidative stress conditions caused by two prooxidants: a superoxide anion generator-menadione (MQ) and hydrogen peroxide (H 2 O 2 ). Methods The levels of enzymatic (catalase, superoxide dismutase, pectinase and β-glucosidase) and nonenzymatic (superoxide anion generator, formaldehyde, phenolic compounds) biomarkers were monitored using biochemical methods in both the supernatants and rhizobial cells after treatment with 0.3mM MQ and 1.5mM H 2 O 2 . The viability of bacterial cells was estimated using fluorescent dyes and confocal laser scanning microscopy. In addition, the effect of prooxidants on symbiosis of the R. leguminosarum bv. trifolii strains with clover was established. Results The tested stress factors significantly changed enzymatic patterns of the rhizobial strains, and the wildtype strain proved to be more resistant to these prooxidants than both pssA and rosR mutants. Significantly higher activities of both catalase and superoxide dismutase have been detected in these mutants in comparison to the wildtype strain. H 2 O 2 and MQ also increased the levels of pectinase and β-glucosidase activities in the tested strains. Moreover, pre-incubation of R. leguminosarum bv. trifolii strains with the prooxidants negatively affected the viability of bacterial cells and the number of nodules elicited on clover plants. Conclusions EPS produced in large amounts by R. leguminosarum bv. trifolii plays a significant protective role as a barrier against oxidative stress factors and during symbiotic interactions with clover plants.

show abstract

The importance of glutathione in oxidative status of Rhizobium leguminosarum biovar viciae under Cd exposure

Cited by 54 publications

References 34 publications

Role of plant growth promoting rhizobacteria in the remediation of metal contaminated soils

Role of plant growth promoting rhizobacteria in the remediation of metal contaminated soils

In vivo antioxidative effect of isoquercitrin on cadmium-induced oxidative damage to mouse liver and kidney

The response of the Rhizobium leguminosarum bv. trifolii wild-type and exopolysaccharide-deficient mutants to oxidative stress

Contact Info

Product

Resources

About