The importance of superoxide anion for Escherichia coli biofilm removal using plasma-activated water

Xia, Baojia; Vyas, Heema Kumari Nilesh; Zhou, Renwu; Zhang, Tianqi; Hong, Jungmi; Rothwell, Joanna G.; Rice, Scott A.; Carter, Dee; Ostrikov, Kostya; Cullen, Patrick J.; Mai‐Prochnow, Anne

doi:10.1016/j.jece.2023.109977

Cited by 21 publications

(8 citation statements)

References 60 publications

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“…Previous research has aptly highlighted the crucial role of ROS within PAW, particularly highlighting the impact of exceptionally long-lived ROS, such as H 2 O 2 , on bacterial survival. The synergistic effect of a low pH and excessive intracellular ROS accumulation triggers redox reactions within the microbial cell, disrupting pH homeostasis and ultimately leading to cell death [11,[47][48][49][50]. The primary inactivation agents are ROS.…”

Section: Discussionmentioning

confidence: 99%

Synergistic Effect of Plasma-Activated Water with Micro/Nanobubbles, Ultraviolet Photolysis, and Ultrasonication on Enhanced Escherichia coli Inactivation in Chicken Meat

Moonsub,

Seesuriyachan,

Boonyawan

et al. 2024

Processes

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The use of integrated plasma-activated water (PAW) with micro/nanobubbles (MNBs), ultraviolet (UV) photolysis, and ultrasonication (US) for the synergistic efficiency of Escherichia coli inactivation in chicken meat was investigated. A 2k factorial design was employed to optimize the combined treatment parameters for pathogen disinfection in Design of Experiments (DOE) techniques. Its effectiveness was evaluated based on electrical conductivity (EC), oxidation–reduction potential (ORP), hydrogen peroxide (H2O2) concentration, and E. coli inactivation. The most significant impact on E. coli reduction was observed for MNBs, UV treatment time, and their interaction (MNBs and UV). Optimal E. coli inactivation (6 log10 CFU/mL reduction) was achieved by combining PAW with MNB and UV for 10 and 20 min, respectively. Integrating PAW with appropriate supplementary technologies enhanced E. coli inactivation by 97% compared to PAW alone. This novel approach provides a promising alternative for pathogen control in chicken meat, potentially improving food safety and shelf life in the poultry industry.

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Section: Discussionmentioning

confidence: 99%

Synergistic Effect of Plasma-Activated Water with Micro/Nanobubbles, Ultraviolet Photolysis, and Ultrasonication on Enhanced Escherichia coli Inactivation in Chicken Meat

Moonsub,

Seesuriyachan,

Boonyawan

et al. 2024

Processes

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“…In fact, scavenging these various ROS from PAW was so effective that E. coli biofilm viability did not significantly differ to the biofilm control. Xia et al [ 28 ] found PAW-associated superoxide was crucial for E. coli biofilm removal, and Rothwell et al [ 19 ] found superoxide (and/or its downstream reactive species) were primary contributors to PAW-mediated inactivation of planktonic E. coli and Listeria innocua cells. Saijai et al [ 44 ] found that ozonated bubble water was a strong sterilising agent against E. coli .…”

Section: Discussionmentioning

confidence: 99%

“…CAP has previously been shown to inactivate bacterial cells by creating an intracellularly high oxidative stress environment with cells responding to this environment by producing additional RONS [ 28 ]. Oxidative stress is harmful to microbial cells and their intracellular components (e.g., nucleic acids, proteins, lipids), and inducing such a surge in intracellular ROS causes irreversible damage and enhances lethality [ 43 , 47 , 48 ].…”

Section: Discussionmentioning

confidence: 99%

“…To further confirm the intracellular accumulation of both ROS and reactive nitrogen species (RNS) upon PAW treatment, biofilms were stained with 20 μM 2′,7′–dichlorofluorescin diacetate (DCFDA; Sigma-Aldrich, Australia) and 5 μmol 4,5-diaminofluorescein diacetate (DAF-FM; Sigma-Aldrich, Australia), respectively [ 28 , 29 ]. Biofilms were challenged for 15 min with 200 μL PAW and control as above.…”

Section: Methodsmentioning

confidence: 99%

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Plasma activated water as a pre-treatment strategy in the context of biofilm-infected chronic wounds

Vyas,

Xia,

Alam

et al. 2023

Biofilm

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“…Many research endeavours have been dedicated to unveiling the bacterial species inhabiting root canals [58][59][60][61]. A substantial body of literature has documented the effective inactivation of bacteria through NTP treatment, underscoring its potent antibacterial efficacy [44,60,62].…”

Section: Discussionmentioning

confidence: 99%

Innovative Curved-Tip Reactor for Non-Thermal Plasma and Plasma-Treated Water Generation: Synergistic Impact Comparison with Sodium Hypochlorite in Dental Root Canal Disinfection

Arguello-Sánchez,

López-Callejas,

Rodríguez-Méndez

et al. 2023

Materials

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Non-thermal plasmas (NTPs), known as cold atmospheric plasmas (CAPs), hold great potential for diverse medical applications, including dentistry. However, traditional linear and rigid dielectric barrier discharge reactors used for NTP generation encounter limitations in accessing oral cavities and root canals. To address this issue, we have developed an innovative NTP reactor featuring an angled end for improved accessibility. The central copper electrode, with a 0.59 mm diameter and adjustable length for desired angulation, is coated with zircon powder (ZrSiO4) to ensure stable NTP generation. This central electrode is housed within a stainless steel tube (3 mm internal diameter, 8 mm external diameter, and 100 mm length) with a 27° angle at one end, making it ergonomically suitable for oral applications. NTP generation involves polarizing the reactor electrodes with 13.56 MHz radio frequency signals, using helium gas as a working medium. We introduce plasma-treated water (PTW) as an adjunctive therapy to enhance biofilm eradication within root canals. A synergistic approach combining NTP and PTW is employed and compared to the gold standard (sodium hypochlorite, NaOCl), effectively neutralizing Enterococcus faecalis bacteria, even in scenarios involving biofilms. Moreover, applying NTP in both gaseous and liquid environments successfully achieves bacterial inactivation at varying treatment durations, demonstrating the device’s suitability for medical use in treating root canal biofilms. The proposed NTP reactor, characterized by its innovative design, offers a practical and specific approach to plasma treatment in dental applications. It holds promise in combatting bacterial infections in root canals and oral cavities.

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The importance of superoxide anion for Escherichia coli biofilm removal using plasma-activated water

Cited by 21 publications

References 60 publications

Synergistic Effect of Plasma-Activated Water with Micro/Nanobubbles, Ultraviolet Photolysis, and Ultrasonication on Enhanced Escherichia coli Inactivation in Chicken Meat

Synergistic Effect of Plasma-Activated Water with Micro/Nanobubbles, Ultraviolet Photolysis, and Ultrasonication on Enhanced Escherichia coli Inactivation in Chicken Meat

Plasma activated water as a pre-treatment strategy in the context of biofilm-infected chronic wounds

Innovative Curved-Tip Reactor for Non-Thermal Plasma and Plasma-Treated Water Generation: Synergistic Impact Comparison with Sodium Hypochlorite in Dental Root Canal Disinfection

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