The importance of the epithelial fibre cell interface to lens regeneration in an in vivo rat model and in a human bag-in-the-lens (BiL) sample

Wu, Weiju; Lois, Noemi; Prescott, Alan R.; Brown, Adrian P.; Gerwen, Veerle Van; Tassignon, Marie-José; Richards, Shane A.; Saunter, Christopher D.; Jarrı́n, Miguel; Quinlan, Roy A.

doi:10.1016/j.exer.2021.108808

Cited by 4 publications

(4 citation statements)

References 94 publications

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“…To establish and maintain the optical symmetry necessary to project images onto the retina, the differentiation of lens cells in their radial layers to form symmetric shells of interconnected fiber cells (314,315) needs to be carefully coordinated both spatially and temporally. The formation of symmetric and concentric spherical layers of elongated denucleated fiber cells in the lens is complex (40), starting with the synchronous proliferation and migration of epithelial cells located in the most distal region of the lens epithelium (118,300,316,317). These are found in the germinative and transitional zones of the epithelium, and their synchronized differentiation is initiated in response to proliferative factors including FGF, insulin-like growth factor, bone morphogenetic protein, and PDGF (283-286, 293, 300, 311, 313, 316).…”

Section: Lens Symmetry and Transparencymentioning

confidence: 99%

Insights into the biochemical and biophysical mechanisms mediating the longevity of the transparent optics of the eye lens

Quinlan

Clark

2022

Journal of Biological Chemistry

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Section: Lens Symmetry and Transparencymentioning

confidence: 99%

Insights into the biochemical and biophysical mechanisms mediating the longevity of the transparent optics of the eye lens

Quinlan

Clark

2022

Journal of Biological Chemistry

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“…The LECs of the anterior capsule were neatly arranged, as were the lens fibers over time, although the transparency of the regenerated lens was lower than that of the primary lens. It has also been suggested that lens cells require spatial and cellular cues to initiate, sustain, and produce optically functional tissue, in addition to capsule integrity and the epithelial-fibrocyte interface (39). In order to improve the transparency of the regenerated lens, some researchers reduced the diameter of the capsulorhexis to 1.0-1.5 mm, the wound area was reduced by 1.2 mm 2 and moved to the periphery of the lens (15).…”

Section: Discussionmentioning

confidence: 99%

The miRNA-34a/Sirt1/p53 pathway in a rat model of lens regeneration

et al. 2022

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Background: There are many molecular factors involved in Wolffian and corneal lens regeneration, but few in lens regeneration by lens epithelial cells (LECs) in mammals. Silent information regulator 1 (Sirt1) has a variety of physiological functions, such as a transport hub, and is involved in pathological conditions.We studied the expression of the microRNA (miRNA)-34a/Sirt1/tumor protein p53 (p53) pathway in a rat model of lens regeneration.Methods: We performed extracapsular lens extraction in 42 healthy female Sprague-Dawley rats. Slit lamp observation was performed at 3, 7, 14, 21, 30, 60 and 90 days postoperatively, and the rats were killed humanely by cervical dislocation at 30, 60 and 90 days postoperatively to remove the eyeballs. We performed semiquantitative immunofluorescence analysis of Sirt1, p53, alpha-smooth muscle actin (α-SMA) and fibronectin (fn), and real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) to detect the relative expressions of miRNA-34a, Sirt1, p53, aquaporin 0 (AQP 0), γA-crystallin, and beaded filament structural protein 1 (BFSP1) mRNA in the lens and posterior capsule. Results:The posterior capsule wrinkled at 3 days and it increased at 7 days. At 14 days, pearl-like opacification appeared under the capsule, with increasing shrinkage. Greater mass-like proliferators in size and number accumulated under the capsule and at the equator after 21 days. A regenerated lens developed in the central depression of the capsule at 30 days, slightly protruding from it. Despite being thickened at 60 days, the central depression persisted, with a smaller change at 90 days than at 60 days. Although the relative mRNA expression of miRNA-34a and p53 in the lens and posterior capsule decreased over time (P=0.000), that of Sirt1 increased (P<0.01). α-SMA was uniformly expressed in the crystals and gradually decreased, while fn expression gradually increased.Conclusions: miRNA-34a expression decreased and Sirt1 expression increased during lens regeneration. Furthermore, p53 expression decreased, thus reducing apoptosis. Therefore, Sirt1 acted as a key factor in the pathway, and played a protective role in lens regeneration.

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“…Purified protein identity was confirmed by proteomic analysis of bands excised after SDS-PAGE (LC-MS analysis) as described previously 49 . Briefly, trypsin was added to the dried, excised band and digestion was performed overnight at 37 0 C. Digestion was stopped by adding trifluoroacetic acid (TFA) and the eluted peptides were cleaned using StageTips as described 50 .…”

Section: Methodsmentioning

confidence: 99%

The major inducible small heat shock protein HSP20-3 in the tardigrade Ramazzottius varieornatus forms filament-like structures and is an active chaperone

Al-Ansari,

Fitzsimons,

Wei

et al. 2024

Cell Stress and Chaperones

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The importance of the epithelial fibre cell interface to lens regeneration in an in vivo rat model and in a human bag-in-the-lens (BiL) sample

Cited by 4 publications

References 94 publications

Insights into the biochemical and biophysical mechanisms mediating the longevity of the transparent optics of the eye lens

Insights into the biochemical and biophysical mechanisms mediating the longevity of the transparent optics of the eye lens

The miRNA-34a/Sirt1/p53 pathway in a rat model of lens regeneration

The major inducible small heat shock protein HSP20-3 in the tardigrade Ramazzottius varieornatus forms filament-like structures and is an active chaperone

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