The Importance of the Hepatic Artery in Rat Liver Transplantation

Howden, B O; Jablonski, Paula; Grossman, Howard; Vc, Marshall

doi:10.1097/00007890-198903000-00005

Cited by 67 publications

(59 citation statements)

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“…In experimental LT in rats, preservation of the hepatic artery is beneficial for longterm outcome. Hepatic rearterialization plays an important role in improving oxygen supply, microvascular perfusion and biliary drainage, minimizing hypoxic and parenchymal cell injury and bile duct complications in rat orthotopic LT (10)(11)(12)(13)(14).…”

Section: Introductionmentioning

confidence: 99%

Hepatic Arterial Perfusion Is Essential for the Spontaneous Recovery From Focal Hepatic Venous Outflow Obstruction in Rats

Huang

Deng

Jin

et al. 2011

American Journal of Transplantation

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We previously observed that focal hepatic venous outflow obstruction recovered spontaneously by the formation of sinusoidal canals in a rat model of portal hyperperfusion. We aimed to investigate whether the lack of hepatic arterial perfusion aggravates parenchymal damage, decelerates recovery and influences the formation of sinusoidal canals after focal hepatic venous outflow obstruction. Rats were subjected to arterialized versus nonarterialized syngeneic liver transplantation after ligating the right median hepatic vein in the donor. Hepatic damage, microcirculation, regeneration and vascular remodeling were evaluated. In arterialized-recipients, confluent necrosis interspersed with viable periportal islands of hepatocytes, and vascularized sinusoidal canals with visible blood flow, surrounded by normal sinusoidal structure, were visible on postoperative day (POD) 2. Complete parenchymal recovery was consequently established by resorption of necrosis and hepatocyte proliferation, detected in viable portal islands and border zone. Lack of hepatic arterial perfusion caused complete necrosis in the obstruction zone without viable hepatocytes in the periportal area on POD2. Hepatocyte proliferation was only visible in the border zone. On POD28, perfused vascular structures, without neighboring normal sinusoidal structures, were observed in the scar-like area. Hepatic arterial perfusion determined the extent of hepatic necrosis, the formation of vascularized sinusoidal canals and the parenchymal recovery, after focal hepatic venous outflow obstruction.

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Section: Introductionmentioning

confidence: 99%

Hepatic Arterial Perfusion Is Essential for the Spontaneous Recovery From Focal Hepatic Venous Outflow Obstruction in Rats

Huang

Deng

Jin

et al. 2011

American Journal of Transplantation

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“…Numerous studies on hepatic arterialization using various reconstruction techniques have been reported 8 , such as an aortic segment-to-aorta anastomosis 3,9,17 , a cuff anastomosis technique 18,19,20 , a telescoping technique 5 , a stent technique 13,16 , and a sleeve anastomosis technique 12,[21][22][23] . While the technique for rat OLT is still not standardized today, the arterialized model has been increasingly favored in terms of its physiological superiority 8,12,13,14 . Among the abovementioned techniques, a stent technique that was simple and fast to perform was reported by Lehmann et al 16 in 2005.…”

Section: Discussionmentioning

confidence: 99%

“…Since rats could survive without hepatic arterial flow after liver transplantation, there was considerable controversy over the value of hepatic arterialization. However, the physiological superiority of the arterialized model has been increasingly acknowledged, especially in terms of preserving the bile duct system 8,12 and the liver integrity 8,13,14 .…”

Section: While Various Techniques and Modifications Of Olt In Rats Hamentioning

confidence: 99%

Surgical Procedures for a Rat Model of Partial Orthotopic Liver Transplantation with Hepatic Arterial Reconstruction

Nagai

Yagi

Üemoto

et al. 2013

JoVE

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Orthotopic liver transplantation (OLT) in rats using a whole or partial graft is an indispensable experimental model for transplantation research, such as studies on graft preservation and ischemia-reperfusion injury 1,2 , immunological responses 3,4 , hemodynamics 5,6 , and small-for-size syndrome 7 . The rat OLT is among the most difficult animal models in experimental surgery and demands advanced microsurgical skills that take a long time to learn. Consequently, the use of this model has been limited. Since the reliability and reproducibility of results are key components of the experiments in which such complex animal models are used, it is essential for surgeons who are involved in rat OLT to be trained in wellstandardized and sophisticated procedures for this model.While various techniques and modifications of OLT in rats have been reported 8 since the first model was described by Lee et al. 9 in 1973, the elimination of the hepatic arterial reconstruction 10 and the introduction of the cuff anastomosis technique by Kamada et al. 11 were a major advancement in this model, because they simplified the reconstruction procedures to a great degree. In the model by Kamada et al., the hepatic rearterialization was also eliminated. Since rats could survive without hepatic arterial flow after liver transplantation, there was considerable controversy over the value of hepatic arterialization. However, the physiological superiority of the arterialized model has been increasingly acknowledged, especially in terms of preserving the bile duct system 8,12 and the liver integrity 8,13,14 .In this article, we present detailed surgical procedures for a rat model of OLT with hepatic arterial reconstruction using a 50% partial graft after ex vivo liver resection. The reconstruction procedures for each vessel and the bile duct are performed by the following methods: a 7-0 polypropylene continuous suture for the supra-and infrahepatic vena cava; a cuff technique for the portal vein; and a stent technique for the hepatic artery and the bile duct. Video LinkThe video component of this article can be found at https://www.jove.com/video/4376/ Protocol Basic Techniques and Common Procedures1. All procedures are carried out under clean but nonsterile conditions. 2. Once the abdomen of the rat is opened, all procedures are performed under a surgical microscope at a magnification of 16x. Exceptions are the ex vivo liver resection, which is performed at 10x, and the following procedures, which are performed at 25x: insertion of a stent into the bile duct and the hepatic artery, and the reconstruction of the hepatic artery, the infrahepatic vena cava (IHVC), and the bile duct. 3. Cotton swabs are used for gentle manipulation of organs, blunt dissection of tissues, and compression hemostasis. Gauze swabs (5 x 5 cm) soaked with lactated Ringer solution are used to retract the liver or intestines, and to keep organs moist. The Satinsky clamp can be used for retraction of gauze-covered intestines toward the left or the tail of the ...

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“…The same problem was thus also found in our microsurgical rat model as a consequence of unrestricted portal venous inflow. Orthotopic liver transplantation in the rat has already been reported [39,40,41,421. The technique with arterialisation of the PV via an AS is based on that described by Engemann [26].…”

Section: Discussionmentioning

confidence: 99%

Arterialisation of the portal vein as a model for the induction of hepatic fibrosis: description of microsurgical models in the rat

Müller

Brummer²,

Erhardt³

et al. 2004

Transplant Int

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The Importance of the Hepatic Artery in Rat Liver Transplantation

Cited by 67 publications

References 0 publications

Hepatic Arterial Perfusion Is Essential for the Spontaneous Recovery From Focal Hepatic Venous Outflow Obstruction in Rats

Hepatic Arterial Perfusion Is Essential for the Spontaneous Recovery From Focal Hepatic Venous Outflow Obstruction in Rats

Surgical Procedures for a Rat Model of Partial Orthotopic Liver Transplantation with Hepatic Arterial Reconstruction

Arterialisation of the portal vein as a model for the induction of hepatic fibrosis: description of microsurgical models in the rat

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