The Improvement of Antimicrobial Activity of Kanamycin and Ciprofloxacin Antibiotics Coupled With Biocompatible Magnetite Nanoparticles and Characterization of Their Structure

Abstract

In this paper, we present the synthesis of nanostructures of magnetite nanoparticles (NPs) with ciprofloxacin and kanamycin antibiotics, based on self-assembling principle. The nanostructures were prepared in crystallite size, ranging 8–16 nm, in one pot addition setup and further washing steps, using only iron precursors and above-mentioned antibiotics as stabilizers. Nanostructures were analyzed by scanning electron microscopy (SEM), X-ray diffraction (XRD) analysis methods, Fourier transform infrared (FTIR) and ultraviolet (UV) spectroscopy methods. It was found that they have well-shaped spherical form and are homogeneous in size. The quantitative analysis of nanostructured antibiotics was performed by atom absorbance spectroscopy (AAS) as well as on the basis of Lambert–Beer law. Prepared nanostructures were tested on Staphylococcus aureus and Pseudomonas aeruginosa. Obtained results demonstrated that these nanostructures are able to improve antimicrobial properties and decrease the minimal inhibitory concentration (MIC) of pristine kanamycin and ciprofloxacin antibiotics.

Metal‐Based Nanomaterials in Biomedical Applications: Antimicrobial Activity and Cytotoxicity Aspects

“…These nanoparticles may be utilized as “Trojan horses” for transferring nanocoupled antibiotics that are usually blocked by the outer membrane into the Gram‐negative bacteria. [ 127 ] Once inside the cytoplasm, dissolution of the antibiotics results in killing the bacteria. In addition to the outer membrane, the periplasmic space between the outer and inner membranes of bacteria contain many enzymatic, heme‐like cytochromes and electron transport chain components for redox reactions that may nullify the action of antibiotics.…”

Synthesis and characterization of β-lactam functionalized superparamagnetic Fe3O4@SiO2 nanoparticles as an approach for improvement of antibacterial activity of β-lactams

“…These nanoparticles have shown signicant attractive for the preparation of immobilized derivative penicillin G, cephalosporin and amoxicillin because of the easy and complete separation from the reaction mixture by applying a magnetic eld. [59][60][61] Using magnetite nanosystems have been investigated to improve the efficiency of current antimicrobials with proved effect. 62 For many applications, magnetic nanoparticles are suitable to be chemically stable and uniform in size to prevent their aggregation, which is happened due to their nanoscale and strong interaction among them.…”

Synthesis, nanostructuring and in silico studies of a new imine bond containing a macroheterocycle as a promising PBP-2a non-β-lactam inhibitor

“…116-118 1C. Synthesis of 6,7, 4,11,14,21,24,31,34]octaoxa [7,18,27,38]tetraazacyclotetracontine (MHC3). 0.37 mmol of dialdehyde 1 was dissolved in 5 mL of hot ethanol.…”

“…Case in point, the biological activity can be very high on the specific strain of the bacteria or be increased in comparison with separate components of the ensemble. [17][18][19] An example of such type of improvement is the nanostructuring of macroheterocyclic drugs rifampicin and rifabutin, which made possible the enhancement of macrophage uptake and antimycobacterial activity. [20][21][22] Different nanoparticles are used for nanostructuring among which magnetite nanoparticles attract special attention.…”