The improvement of fibroblast growth on hydrophobic biopolyesters by coating with polyhydroxyalkanoate granule binding protein PhaP fused with cell adhesion motif RGD

Cartilage Tissue Engineering Using Mesenchymal Stem Cells and 3D Chitosan Scaffolds – In vitro and in vivo Assays

“…Strong cell attachment and proliferation demonstrated that there was no cytotoxicity in either scaffolds used. The results agreed well with previous studies showing that initial cell adhesion was largely influenced of RGD in gelatin bound to chitosan (Dong et al, 2010). It was reported that immobilization of RGD peptide onto a scaffold enabled the adhesion of stem cells to the scaffold and inhibited the immediate matrix-induced cell aggregation (Re'em et al, 2010).…”

“…It is acid soluble, forms like-gel solutions and is water insoluble. Therefore, chitosan is available in nature and easily manipulated beyond its seemed ECM polysaccharides state (Roughley et al, 2006 andDong et al, 2010). Gelatin is derived from collagen, mainly proteins presents in cartilage ECM.…”

Polymer films with surfaces unmodified and modified by non-thermal plasma as new substrates for cell adhesion

“…Other authors have reported the use of SEM images to study the morphology of fibroblasts adhered to different materials [35][36][37]. Thus, the cell morphology, with easy identification of cell components, was easily observed in this study, especially in the 1:1 PS/PMMA film.…”

Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)-based scaffolds for tissue engineering

“…Arginine-glycine-aspartate (RGD) tripeptide is normally involved in cell binding to a number of ECM glycoproteins by interaction with integrins (45). The PhaP-RGD fusion protein, when used to coat PHBHHx, has been shown to enhance cell adhesion, spreading, migration, and proliferation (31). Similarly, You et al (5) showed that PhaP-RGD-coated PHBHHx scaffolds facilitate chondrogenic differentiation of human bone marrow mesenchymal stem cells inoculated onto them.…”