The Improvement of Porcine In Vitro Embryo Development through Regulating Autophagy by miRNA-143 Inhibition

Ridlo, Muhammad Rosyid; Kim, Eui Hyun; Kim, Eun Pyo

doi:10.3390/ani12192651

Cited by 3 publications

(2 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, Li et al [ 89 ] reported that miR-451 downregulation in mouse and human oocytes affected pre-implantation embryogenesis by suppressing the Wnt signaling pathway, suggesting that miR-451 might serve as a novel biomarker of oocyte and embryo quality in ARTs. Another miRNA increased in BGE was miR-143 which has been found as involved in porcine embryo development [ 90 ]. By contrast, miR-184, with a role in supporting embryo development as mentioned above, showed lower abundance in good vs. poor embryos during co-culture with BOEC.…”

Section: Discussionmentioning

confidence: 99%

Oviductal extracellular vesicles miRNA cargo varies in response to embryos and their quality

Hamdi,

Sánchez,

Fernandez-Fuertes

et al. 2024

BMC Genomics

View full text Add to dashboard Cite

Background Increasing evidence points to an active role of oviductal extracellular vesicles (oEVs) in the early embryo-maternal dialogue. However, it remains unclear whether oEVs contribute to the recognition of the presence of embryos and their quality in the oviduct. Hence, we examined whether the molecular cargo of oEVs secreted by bovine oviduct epithelial cells (BOEC) differs depending on the presence of good (≥ 8 cells, G) or poor (< 8 cells, P) quality embryos. In addition, differences in RNA profiles between G and P embryos were analyzed in attempt to distinguish oEVs and embryonic EVs cargos. Methods For this purpose, primary BOEC were co-cultured with in vitro produced embryos (IVP) 53 h post fertilization as follows: BOEC with G embryos (BGE); BOEC with P embryos (BPE); G embryos alone (GE); P embryos alone (PE); BOEC alone (B) and medium control (M). After 24 h of co-culture, conditioned media were collected from all groups and EVs were isolated and characterized. MicroRNA profiling of EVs and embryos was performed by small RNA-sequencing. Results In EVs, 84 miRNAs were identified, with 8 differentially abundant (DA) miRNAs for BGE vs. B and 4 for BPE vs. B (P-value < 0.01). In embryos, 187 miRNAs were identified, with 12 DA miRNAs for BGE vs. BPE, 3 for G vs. P, 8 for BGE vs. GE, and 11 for BPE vs. PE (P-value < 0.01). Conclusions These results indicated that oEVs are involved in the oviductal-embryo recognition and pointed to specific miRNAs with signaling and supporting roles during early embryo development.

show abstract

Section: Discussionmentioning

confidence: 99%

Oviductal extracellular vesicles miRNA cargo varies in response to embryos and their quality

Hamdi,

Sánchez,

Fernandez-Fuertes

et al. 2024

BMC Genomics

View full text Add to dashboard Cite

show abstract

“…The proper timing of 6 h after the electrical activation of the oocytes has been explained previously (Ridlo et al, 2021(Ridlo et al, , 2022. At 6 h after activation, the zygotes were held with a holding pipette in a 4-μl drop of PZM-5.…”

Section: Microinjection 6 H After Electrical Activationmentioning

confidence: 99%

Effects of klotho protein or klotho knockdown in porcine oocytes at different stages

Kim,

Park

et al. 2023

Zygote

View full text Add to dashboard Cite

Summary Klotho is a protein that plays different functions in female fertility. We have previously reported that klotho protein supplementation during in vitro maturation improves porcine embryo development, while klotho knockout for somatic cell cloning completely blocks full-term pregnancy in vivo. However, the effects of the microinjection of klotho protein or klotho knockdown dual vector in porcine embryos at different time points and the specific molecular mechanisms remain largely unknown. In this study, we injected the preassembled cas9 + sgRNA dual vector, for klotho knockdown, into the cytoplasm of the germinal vesicle stage of oocytes and into porcine embryos after 6-h parthenogenetic activation. Similarly, the klotho protein was inserted into the cytoplasm of germinal vesicle stage oocytes and porcine embryos after 6-h parthenogenetic activation. Compared with the controls, the microinjection of klotho dual vector markedly decreased the blastocyst formation rates in germinal vesicle stage oocytes and activated embryos. However, the efficiency of blastocyst formation when klotho protein was inserted before in vitro maturation was significantly higher than that after klotho protein insertion into parthenogenetically activated embryos. These results indicated that klotho knockdown may impair embryo development into blastocyst irrespective of injection timing. In addition, klotho protein injection timing in pig embryos may be an important factor for regulating embryo development.

show abstract

Circular RNAs from bovine blastocysts can interact with miRNAs/tsRNAs from embryonic extracellular vesicles and regulate hatching

Fan,

Pavani,

Broeckx

et al. 2024

International Journal of Biological Macromolecules

View full text Add to dashboard Cite

The Improvement of Porcine In Vitro Embryo Development through Regulating Autophagy by miRNA-143 Inhibition

Cited by 3 publications

References 33 publications

Oviductal extracellular vesicles miRNA cargo varies in response to embryos and their quality

Oviductal extracellular vesicles miRNA cargo varies in response to embryos and their quality

Effects of klotho protein or klotho knockdown in porcine oocytes at different stages

Circular RNAs from bovine blastocysts can interact with miRNAs/tsRNAs from embryonic extracellular vesicles and regulate hatching

Contact Info

Product

Resources

About