The in vitro development of human zygote reconstructed by pronuclear transfer

First birth following spindle transfer for mitochondrial replacement therapy: hope and trepidation

“…A number of these publications are cited. Included in their pre-clinical studies are limited data that suggest a 'modified electrofusion' technique does not lead to increased aneuploidy levels following human pronuclear transfer (PNT) (Liu et al, 2015). But it is not clear how modifications such as opening the zona pellucida using laser ablation (rather than zona dissection) and spindle transfer at 37°C (rather than at room temperature) can address concerns with electrofusion, as the authors do not present any experimental data to support their claim.…”