The in vitro effects of Bordetella pertussis lymphocytosis-promoting factor on murine lymphocytes. III. B-cell dependence for T-cell proliferation.

M, Ho; Kong, A S; Morse, Stephen I.

doi:10.1084/jem.149.5.1001

Cited by 21 publications

(13 citation statements)

References 42 publications

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“…Recently, we have demonstrated the enhancing effect of B lymphocytes as well as macrophages on T cell responses to antigen in the guinea pig [22], Similar results were presented by Romagnani et al [23] and Kasahara et al [24] using a T cell mitogen in man. Ho et al [25] also confirmed the requirement for B helper cells in murine T cell proliferation induced by Bordetella pertussis Imyphocytosis-promoting factor. It is presumed that B lymphocytes, probably acting as antigenpresenting and/or antigen-processing cells, can induce an antigen specific T cell prolif erative response [26,27], On the other hand, purified B lymphocytes did not re spond to NAE even in the presence of mito mycin C-treated macrophages.…”

Section: Discussionsupporting

confidence: 55%

Development of Cell-Mediated Hypersensitivity in Guinea Pigs following Infection with Nocardia asteroides

Hiramine¹,

Hojo²,

Yano³

1981

Int Arch Allergy Immunol

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Partially purified protein antigen which was prepared from cells of Nocardia asteroides was used to assess the immune responsiveness of guinea pigs inoculated subcutaneously with living N. asteroides. Cell-mediated reactivity, as measured by in vitro lymphocyte transformation of and blastogenic factor production by lymph node cells as well as a delayed skin reaction, appeared clearly at 1 week after N. asteroides infection, when an early abscess was formed at the inoculated site. The in vitro proliferative response of lymph node cells reached a maximum at 2 weeks at which time the lesion of the inoculated site showed central suppuration surrounded by epithelioid and giant cells. The lymphocyte transformation response then declined rapidly but a significant response continued until 6 weeks after infection. The production of blastogenic factor was maximum at 3 weeks, but continued till 6 weeks. A high level of skin reactivity was maintained until 6 weeks. By 6 weeks after infection the lesion at the inoculated site usually resolved and disappeared. Thus, enhancement of cell-mediated reactivity coincided with the time of granulomatous conversion of an early suppurative abscess at the site of inoculation of Nocardia organisms. In studies on the lymphocyte proliferative response in vitro of N. asteroides-infected animals, purified lymph node T cell populations failed to respond to Nocardia antigen, but they could be induced to proliferate in the presence of autologous macrophages or B cells. Purified B lymphocytes, though unresponsive to Nocardia antigen by themselves or following the addition of macrophages, were stimulated by Nocardia antigen in the presence of T cells. The proliferative response of lymph node cells to Nocardia antigen seems to be the result of a collaborative event that occurs between T cells and macrophages or T and B cells.

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Section: Discussionsupporting

confidence: 55%

Development of Cell-Mediated Hypersensitivity in Guinea Pigs following Infection with Nocardia asteroides

Hiramine¹,

Hojo²,

Yano³

1981

Int Arch Allergy Immunol

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“…However, although PT is a T cell mitogen, macrophages are not essential for the ex pression of mitogenicity [4], Therefore, we believe that in the process of T cell activation by PT the macro phage requirement may be relatively lower, but we have no direct evidence.…”

Section: Discussionmentioning

confidence: 69%

Activation of Distinct T Cell Subsets Involved in IgM Antibody Response of Mice by Pertussis Toxin from Bordetella pertussis Strain Maeno

Kumazawa

Yamada

Ohtani

et al. 1984

Int Arch Allergy Immunol

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T cell activation mechanisms in the spleen induced by pertussis toxin (PT) were investigated. The degree of T cell activation was judged from results of helper activity assessed by measuring the in vitro anti-trinitrophenyl (TNP) IgM antibody response. When mice were injected intraperitoneally with 4 × 10⁵ sheep erythrocytes (SRBC) and concomitantly given an intravenous injection with 10 µg of PT 4 days previously, significant generation of nonspecific helper T cells was observed unless primed T cells were stimulated in vitro with TNP-SRBC. Stimulation with TNP-SRBC resulted in marked suppression of helper activity because it induced generation of suppressor T cells specific for the carrier antigen.

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“…(However, the expression of cellular cytotoxic activity does not require DNA synthesis because mitomycin C did not block the activity of LPF-stimulated ceils.) In preliminary experiments, it also appears that the generation of CEL by LPF, like the proliferative T-cell response, is completely dependent upon the presence of a nonproliferating accessory sIg + cell (B cell) (13). Interestingly, the kinetics of maximal 3H-thymidine incorporation and the generation of cytotoxic cells were different.…”

Section: Effector: Target Cell Ratiomentioning

confidence: 97%

The in vitro effects of Bordetella pertussis lymphocytosis-promoting factor on murine lymphocytes. IV. Generation, characterization, and specificity of cytotoxic lymphocytes.

Kong¹,

Morse²

1979

The Journal of Experimental Medicine

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Cytotoxic effector lymphocytes were induced in cultures of mouse spleen or lymph node cells by lymphocytosis promoting factor (LPF). The LPF-activated cytotoxic cells: (a) were not generated unless proliferation occurred; (b) sedimented in the lighter density fraction of a bovine serum albumin gradient; (c) were large, blast-like cells; and (d) were lysed by Thy-1.2 antiserum plus complement and, therefore, were T cells. Neither LPF alone nor supernates from stimulated cultures were cytotoxic. Unlike the situation with concanavalin A and phytohemagglutinin P, LPF-stimulated cytotoxic effector lymphocytes required no further addition of mitogen for maximal cytotoxicity. The effector cells displayed specificity, destroying only allogeneic but not syngeneic normal cells; in the case of tumor cells, both allogeneic and syngeneic cells werelysed in the absence of added mitogen. The reason for differentiated cytotoxicity toward syngeneic tumor and normal cells is not clear but may have some relevance to in vivo tumor rejection initiated by Bordetella pertussis.

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The in vitro effects of Bordetella pertussis lymphocytosis-promoting factor on murine lymphocytes. III. B-cell dependence for T-cell proliferation.

Cited by 21 publications

References 42 publications

Development of Cell-Mediated Hypersensitivity in Guinea Pigs following Infection with <i>Nocardia asteroide</i><i>s</i>

Development of Cell-Mediated Hypersensitivity in Guinea Pigs following Infection with <i>Nocardia asteroide</i><i>s</i>

Activation of Distinct T Cell Subsets Involved in IgM Antibody Response of Mice by Pertussis Toxin from <i>Bordetella pertussis </i>Strain Maeno

The in vitro effects of Bordetella pertussis lymphocytosis-promoting factor on murine lymphocytes. IV. Generation, characterization, and specificity of cytotoxic lymphocytes.

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