The in vitro effects of Bordetella pertussis lymphocytosis-promoting factor on murine lymphocytes. IV. Generation, characterization, and specificity of cytotoxic lymphocytes.

Abstract

Cytotoxic effector lymphocytes were induced in cultures of mouse spleen or lymph node cells by lymphocytosis promoting factor (LPF). The LPF-activated cytotoxic cells: (a) were not generated unless proliferation occurred; (b) sedimented in the lighter density fraction of a bovine serum albumin gradient; (c) were large, blast-like cells; and (d) were lysed by Thy-1.2 antiserum plus complement and, therefore, were T cells. Neither LPF alone nor supernates from stimulated cultures were cytotoxic. Unlike the situation with concanavalin A and phytohemagglutinin P, LPF-stimulated cytotoxic effector lymphocytes required no further addition of mitogen for maximal cytotoxicity. The effector cells displayed specificity, destroying only allogeneic but not syngeneic normal cells; in the case of tumor cells, both allogeneic and syngeneic cells werelysed in the absence of added mitogen. The reason for differentiated cytotoxicity toward syngeneic tumor and normal cells is not clear but may have some relevance to in vivo tumor rejection initiated by Bordetella pertussis.

Effect of Histamine‐Sensitizing Factor of Bordetella pertussis on Pharmacologic Response of Rat Atria

“…Although there are many reports on the reaction in vivo, there have been only a limited number of basic studies at the tissue or cellular level in vitro for analyzing the nature of the toxicity. It has been found in vitro that HSF acts as a mitogen for mouse and human lymphocytes (1, 7,8,12), as well as an inducer for cytotoxic effector lymphocytes in cultures of mouse spleen or lymph node cells (9), and that it depresses the increase in the level of cAMP in human lymphocytes induced by epinephrine or prostaglandin (10). Recently, we found that HSF stimulates rat adipocytes to release glycerol in vitro (3).…”

Glycerol‐Releasing Activity of Histamine‐Sensitizing Factor of Bordetella pertussis for Rat Adipocytes In Vitro

“…Although many studies on the action of HSF in vivo have been reported, little is known about its mode of action, especially at the cellular or tissue level. Andersen et al (1), and Kong and Morse (II,12) reported that LPS acts as a mitogen for mouse and human lymphocytes in vitro, and Kong and Morse (13) showed that LPF induces cytotoxic effector lymphocytes in cultures of mouse spleen or lymph node cells. Lee (14) reported that HSF depresses the cyclic nucleotide level increased by epinephrine or prostaglandin in human lymphocytes in vitro.…”