The in vitro effects of semiconductor laser irradiation on inflammation: laser irradiation did not affect monocyte infiltration

Abstract

The effects of supernatants following irradiation of lymphocytes and monocytes with a semiconductor laser, on monocyte chemotaxis were investigated in vitro. Human peripheral lymphocytes and monocytes were obtained from healthy adult donors, and were suspended in medium, following laser irradiation. The laser irradiation (wavelength 900 nm, output power 1.5 mW) was carried out in the pulse wave mode at doses of 0.1 J cm -2 in total. After irradiation, the lymphocytes and monocytes were incubated with or without several kinds of stimulants for varying time periods. The supernatants of these cultures were then assayed for human monocyte chemotaxis. Through these experiments, we were unable to detect any significant levels of stimulative or depressive effects due to laser irradiation of the lymphocytes and monocytes, on the production of monocyte chemotactic activity.

Actinobacillus actinomycetemcomitans serotype b-specific polysaccharide antigen stimulates production of chemotactic factors and inflammatory cytokines by human monocytes

“…cells) were cultured in triplicate in 1 ml of Dulbecco's modified Eagle medium (DMEM; GIBCO Laboratories, Grand Island, N.Y.) containing 100 IU of penicillin per ml, 100 g of streptomycin per ml, and 2 mM L-glutamine in a 24-well plate (Falcon 3047; Becton Dickinson and Co., Paramus, N.J.) in a humidified atmosphere of 95% air and 5% CO 2 at 37ЊC. After 2 h of incubation, the nonadherent cells were removed by washing with DMEM, leaving a population of Ͼ95% phagocytic cells (40,48). These adherent monolayers were used as human monocytes.…”

“…Chemotaxis assay was performed by using a 96-well chemotaxis assembly (Neuro Probe, Rockville, Md.) (48). FMLP was used as a positive control for chemotaxis assay.…”