The in vitro germination and storage characteristics of Keteleeria fortunei var. cyclolepis pollen provide a reference for cross breeding

Germination Characteristics and Dynamic Changes of Antioxidant Enzymes during Storage of Viola dissecta Pollen

“…At -20 and -80 o C, the germination rate of V. dissecta pollen was 45.3% after 365 d storage at -80℃, which was 53.17% of that before storage, the activities of SOD, POD and CAT were more than 80% before storage, the finding was in good agreement with that reported for Keteleeria fortunei pollen (Liu et al 2020). This indicated that pollen has the ability to scavenge active oxygen and free radicals, and can delay pollen senescence (Xu et al 2015).…”

“…The storage pollen at -20°C in sweet cherry had greater pollen viability rather than pollen those at -80 or 4°C (Özcan 2020). Contrasting results have been reported by Liu et al (2020) and Jia et al (2015) that pollen germination in Keteleeria fortunei and Paeonia qiui was superior at -80°C than other temperatures. We found that pollen stored at room temperature, 4 or -20°C only had a low germination rate compared with those stored at -80°C in V. dissecta.…”

“…The level of antioxidant enzyme activity can reflect pollen germination ability to a certain extent (Mao et al 2016;Guo et al 2017;Ren et al 2021). During pollen storage, the rapid decrease of antioxidant enzyme activity after reaching the peak often indicates a rapid decline in pollen germination rate (Akhond et al 2000;Abdelgadir et al 2012;Dong et al 2017;Liu et al 2020). Our study showed that at 4°C, the time when the pollen germination rate drops to 0% was delayed by 80 d compared with that at room temperature.…”

“…The pollen preservation is of great importance for breeding new varieties through hybridization (Keller et al 1996;Báez et al 2002), low moisture content and low temperature can effectively reduce the physiological activity and nutrient consumption during pollen storage, and extend pollen longevity (Tan 2011;Liu et al 2020). The storage pollen at -20°C in sweet cherry had greater pollen viability rather than pollen those at -80 or 4°C (Özcan 2020).…”

Optimization of culture medium for in vitro germination and storage conditions of Exochorda racemosa pollen

“…These observations were similar to pollen germination from six male varieties of P. dactylifera, with a significantly faster reduction in in vitro germination at 25°C compared to pollen stored at 4°C or −20°C (Mesnoua et al, 2018b). Jia et al (2020) and Liu et al (2020) also reported that pollen viability was significantly higher after −20°C storage than 4°C storage. Our results are also consistent with those of Liu et al (2020) and Jia et al (2020), where a higher pollen in vitro germination rate was obtained at −20°C, followed by lower germination rates following storage at 4°C and 25°C (Maryam et al, 2015;Jaskani and Naqvi, 2017).…”