The in vitro research of bacterial invasion of prosthetic vascular grafts: comparison of elastomer-sealed and gelatin-coated Dacron vascular grafts

Sasaki, Yuki

doi:10.1007/s00595-013-0761-8

Cited by 7 publications

(3 citation statements)

References 11 publications

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“…A common inquiry over the use of gelatin as biomaterials is whether this polymer is prone to bacterial infection (Sasaki 2014), given its use as a medium component for bacterial culture (Bezrukikh et al 2014). To date, there is no solid evidence to support this argument and sterile gelatin is generally considered safe.…”

Section: Drawbacks and Challengesmentioning

confidence: 99%

Recent advances in the use of gelatin in biomedical research

2015

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The biomacromolecule, gelatin, has increasingly been used in biomedicine-beyond its traditional use in food and cosmetics. The appealing advantages of gelatin, such as its cell-adhesive structure, low cost, off-the-shelf availability, high biocompatibility, biodegradability and low immunogenicity, among others, have made it a desirable candidate for the development of biomaterials for tissue engineering and drug delivery. Gelatin can be formulated in the form of nanoparticles, employed as size-controllable porogen, adopted as surface coating agent and mixed with synthetic or natural biopolymers forming composite scaffolds. In this article, we review recent advances in the versatile applications of gelatin within biomedical context and attempt to draw upon its advantages and potential challenges.

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Section: Drawbacks and Challengesmentioning

confidence: 99%

Recent advances in the use of gelatin in biomedical research

2015

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“…The author reported the possibility that bacteremia might induce infection around the vascular graft through bacterial invasion of the graft. 8) The mechanism of infection in our case is unlikely to be related to SSI in the light of the onset timing of 4 years postoperatively. In this case, since a persistent slight CRP increase almost normalized with oral antibiotic therapy continued for several months during the postoperative course of the first surgery, a localized latent SSI with weak pathogenic bacteria may have induced abscess formation and prosthetic vascular graft infection upon activation of the bacteria due to changes in the host.…”

Section: Discussionmentioning

confidence: 60%

A Case of an Aortic Abscess around the Elephant Trunk

Fujii

Kawasaki

Katayanagi

et al. 2015

ATCS

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A 52-year-old male patient with a history of total arch replacement using the elephant trunk technique for acute aortic dissection 4 years before visited our hospital with the chief complaint of persistent fever. Chest computed tomography (CT) suggested prosthetic vascular graft infection, which was treated surgically after chemotherapy. The first surgery consisted of debridement of an abscess around the vascular graft and in the aorta around the elephant trunk, and thoracic descending aorta replacement and vacuum-assisted closure (VAC) in view of the risk of bleeding from the peripheral region of the elephant trunk. One week later, omental filling was performed as the second step. This is a very rare case of aortic abscess around the elephant trunk that could successfully be managed by graft-conserving treatment.

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“…In the experimental setup, 12 or 18 grafts were cut into 6-cm segments, placed in a U-shaped configuration on culture plates and inoculated at the outer graft surface. Data revealed a bacterial invasion through the prosthetic graft material starting 6 h post-bacterial immersion with no significant differences between the two different treatments of the outer polyester surface (Sasaki, 2014). …”

Section: Discussionmentioning

confidence: 99%

Rapid in Vitro Quantification of S. aureus Biofilms on Vascular Graft Surfaces

et al. 2017

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Objectives: Increasing resistance of microorganisms and particularly tolerance of bacterial biofilms against antibiotics require the need for alternative antimicrobial substances. S. aureus is the most frequent pathogen causing vascular graft infections. In order to evaluate the antimicrobial efficacy, quantification of the bacterial biofilms is necessary. Aim of the present study was the validation of an in vitro model for quantification of bacterial biofilm on vascular graft surfaces using three different assays.Methods: Standardized discs of vascular graft material (Dacron or PTFE) or polystyrene (PS) as control surface with 0.25 cm2 surface area were inoculated with 10−3 diluted overnight culture of three biofilm-producing S. aureus isolates (BEB-029, BEB-295, SH1000) in 96-well PS culture plates. After incubation for 4 and 18 h, the biofilm was determined by three different methods: (a) mitochondrial ATP concentration as measure of bacterial viability (ATP), (b) crystal violet staining (Cry), and (c) vital cell count by calculation of colony-forming units (CFU). The experiments were performed three times. Quadruplicates were used for each isolate, time point, and method. In parallel, bacterial biofilms were documented via scanning electron microscopy.Results: All three methods could quantify biofilms on the PS control. Time needed was 0:40, 13:10, and 14:30 h for ATP, Cry, and CFU, respectively. The Cry assay could not be used for vascular graft surfaces due to high unspecific background staining. However, ATP assay and CFU count showed comparable results on vascular graft material and control. The correlations between ATP and CFU assay differed according to the surface and incubation time and were significant only after 4 h on Dacron (BEB-029, p = 0.013) and on PS (BEB-029, p < 0.001). Between ATP and Cry assay on PS, a significant correlation could be detected after 4 h (BEB-295, p = 0.027) and after 18 h (all three strains, p < 0.026). The reproducibility of the ATP-assay presented as inter-assay-variance of 2.1 and as intra-assay variance of 8.1 on polystyrene.Conclusion: The in-vitro model reproducibly quantifies biofilm on standardized vascular graft surfaces with ATP assay as detection system. The ATP assay allows accelerated microbial quantification, however the correlation with the CFU assay may be strain- and surface-dependent.

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The in vitro research of bacterial invasion of prosthetic vascular grafts: comparison of elastomer-sealed and gelatin-coated Dacron vascular grafts

Cited by 7 publications

References 11 publications

Recent advances in the use of gelatin in biomedical research

Recent advances in the use of gelatin in biomedical research

A Case of an Aortic Abscess around the Elephant Trunk

Rapid in Vitro Quantification of S. aureus Biofilms on Vascular Graft Surfaces

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