The Inactivation of Virus in Cultured Shoot Tips of Nicotiana rustica L.

Abstract

SUMMARYThree weeks after inoculation, cherry leaf roll virus and arabis mosaic virus were each detected in the apices of at least seven axillary buds above the inoculated leaves of Nieotiana rustica plants. Such infected apices were grown into young plants on Linsmaier and Skoog's (1965) medium, and their virus content tested after 14 to I35 days; 5 ° to 78 % were apparently free from cherry leaf roll virus and 69 % from arabis mosaic virus. In a more detailed test with cherry leaf roll virus, the proportion of virus-free plants increased from 50% after 16 days to IOO% after io8 days. Similar plants grown for 3o weeks remained free of symptoms and detectable virus.

Propagation of plant viruses in hairy root cultures: A potential method for in vitro production of epitope vaccines and foreign proteins

“…Although cultured roots contain vascular tissue, transpiration-driven convection does not occur in submerged cultures and this would be expected to reduce the rate of viral spread compared with intact plants. In addition, the ability of the cultures to accumulate TMV may be restricted by the large number of lateral meristems generally developed by hairy roots and the inability of plant viruses to replicate and accumulate in meristemic tissue (Valentine et al, 2002;Walkey et al, 1969).…”