The incidence of cytogenetically abnormal rogue cells in peripheral blood

Mustonen, R; Lindholm, Carita; Ej, Tawn; Sabatier, Laure; Salomaa, Sisko

doi:10.1080/095530098141069

Cited by 19 publications

(5 citation statements)

References 15 publications

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“…A random sample of ten veterans showed a total of 37 CCR cells, whereas the number of CCR cells observed in the entire control group amongst the thousands of cells observed, totaled less than ten. There is general agreement in the literature that there is no correlation between exposure to ionizing radiation per se and the occurrence of rogue cells, except possibly in one study on astronauts exposed to high-LET radiation (Mustonen et al, 1998). Nonetheless, the presence of CCRs in veterans may be viewed as an additional indicator of past radiation exposure.…”

Section: Resultsmentioning

confidence: 99%

Elevated chromosome translocation frequencies in New Zealand nuclear test veterans

Wahab

Nickless

Najar-M’Kacher

et al. 2008

Cytogenet Genome Res

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In 1957/58 the British Government conducted a series of nuclear tests in the mid-Pacific codenamed Operation Grapple, which involved several naval vessels from Britain and New Zealand. Two New Zealand frigates with 551 personnel onboard were stationed at various distances between 20 and 150 nautical miles from ground zero. In the present study we applied the cytomolecular technique mFISH (multicolour fluorescent in situ hybridisation) to investigate a potential link between chromosome abnormalities and possible past radiation exposure in New Zealand nuclear test veterans who participated in Operation Grapple. Compared to age matched controls, the veterans showed significantly higher (P < 0.0001) frequencies of chromosomal abnormalities (275 translocations and 12 dicentrics in 9,360 cells vs. 96 translocations and 1 dicentric in 9,548 cells in the controls), in addition to a significant excess of CCRs (complex chromosomal rearrangements) in the veterans. A Kolmogorov-Smirnoff test showed that the distributions of translocations for the two groups were significantly different.

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Section: Resultsmentioning

confidence: 99%

Elevated chromosome translocation frequencies in New Zealand nuclear test veterans

Wahab

Nickless

Najar-M’Kacher

et al. 2008

Cytogenet Genome Res

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“…Such rogue cells, as termed by Awa and Neel (1), were found -similar to the present observations -both from controls and from radiation exposed subjects. For example, collecting the data on rogue cells from a total of nine cytogenetic studies on radiation exposed populations and controls from three laboratories in the United Kingdom, France and Finland, Mustonen et al (27) found the highest incidence of these cells in a control group of young trainees (1 : 400), whereas the lowest incidence of rogue cells (1 : 36 500) was demonstrated in a follow-up study of people accidentally exposed to high levels of ionising radiation. Therefore, the present incidence of rogue cells (1 : 7800) cannot be associated with RSO treatment.…”

Section: Discussionmentioning

confidence: 99%

Biological dosimetry after radiosynoviorthesis with rhenium-186 sulphide and erbium-169 citrate

Klett¹,

Schnurbus-Duhs²,

Mödder³

et al. 2011

Nuklearmedizin

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“…“Rogue cells” are cells with multiple and complex chromosomal aberrations (e.g., dicentric, tricentric, translocations, insertions, deletions, and acentric chromosomes) related to the activation of viral infection [ 100 , 101 ]. A significant increase in induced chromosomal aberrations has been detected in the presence of rogue cells [ 102 , 103 , 104 , 105 ]. Further studies are needed to investigate the role of viral infection in the formation of radiation-induced chromosomal aberrations.…”

Section: Advantages and Limitations Of Current Cytogenetic Biomarkers...mentioning

confidence: 99%

High Resolution and Automatable Cytogenetic Biodosimetry Using In Situ Telomere and Centromere Hybridization for the Accurate Detection of DNA Damage: An Overview

M’Kacher

Colicchio

Junker

et al. 2023

IJMS

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In the event of a radiological or nuclear accident, or when physical dosimetry is not available, the scoring of radiation-induced chromosomal aberrations in lymphocytes constitutes an essential tool for the estimation of the absorbed dose of the exposed individual and for effective triage. Cytogenetic biodosimetry employs different cytogenetic assays including the scoring of dicentrics, micronuclei, and translocations as well as analyses of induced premature chromosome condensation to define the frequency of chromosome aberrations. However, inherent challenges using these techniques include the considerable time span from sampling to result, the sensitivity and specificity of the various techniques, and the requirement of highly skilled personnel. Thus, techniques that obviate these challenges are needed. The introduction of telomere and centromere (TC) staining have successfully met these challenges and, in addition, greatly improved the efficiency of cytogenetic biodosimetry through the development of automated approaches, thus reducing the need for specialized personnel. Here, we review the role of the various cytogenetic dosimeters and their recent improvements in the management of populations exposed to genotoxic agents such as ionizing radiation. Finally, we discuss the emerging potentials to exploit these techniques in a wider spectrum of medical and biological applications, e.g., in cancer biology to identify prognostic biomarkers for the optimal triage and treatment of patients.

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The incidence of cytogenetically abnormal rogue cells in peripheral blood

Cited by 19 publications

References 15 publications

Elevated chromosome translocation frequencies in New Zealand nuclear test veterans

Elevated chromosome translocation frequencies in New Zealand nuclear test veterans

Biological dosimetry after radiosynoviorthesis with rhenium-186 sulphide and erbium-169 citrate

High Resolution and Automatable Cytogenetic Biodosimetry Using In Situ Telomere and Centromere Hybridization for the Accurate Detection of DNA Damage: An Overview

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