The incidence of sister chromatid exchanges in cultured human lymphocytes

Morgan, William F.; Crossen, Peter E.

doi:10.1016/s0027-5107(77)80032-8

Cited by 105 publications

(26 citation statements)

References 20 publications

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“…The increased effects in children of exposed mothers suggest that active agents might be able to pass through the placenta and produce lesions in lymphocytes which may remain latent for years. However, contrary to other studies (26,31) adult controls had higher mean SCE frequencies that did control children. Detailed interviews revealed that many control adults previously had diagnostic x-rays, had taken different drugs for a variety of disorders, and were smokers.…”

contrasting

confidence: 99%

“…Variation within control subjects, examined by comparison of duplicate cultures (23,31) or of repeat cultures from the same individual taken at 3 month intervals (31) revealed no significant differences. Likewise, no significant differences were apparent between sexes (20,31,34) or with age from 0 to 85 yr (26,31).…”

mentioning

confidence: 89%

“…A summary of baseline values as determined in normal control subjects (> 10 individuals/study and approximately 20 cells/individual) in various laboratories (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33) is illustrated in Figure 2. Possible factors contributing to the observed variation between laboratories might be due to: real differences in methods of culturing and/or scoring cells or real differences in the populations studied.…”

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confidence: 99%

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Chromosomal methods in population studies.

Conner¹,

Wald²

1981

Environ Health Perspect

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A brief description of chromosome aberrations and sister chromatid exchange (SCE) as cytogenetic endpoints for evaluation of DNA damaging agents is presented. Problems associated with the use of cytogenetic assays as population monitors of radiation and chemical exposures are discussed. Adequate cell sample size requirements and accurate assessment of cummulative exposure effects with increasing age are stressed as important considerations for reliable cost-benefit analysis of population studies involving low level exposures. Examples of population studies using SCE as an indicator of specific chemical exposures are cited, and factors contributing to variations in control baseline SCE levels are discussed. Possible implications of population cytogenetic data on general public health are suggested.

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confidence: 99%

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confidence: 89%

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confidence: 99%

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Chromosomal methods in population studies.

Conner¹,

Wald²

1981

Environ Health Perspect

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“…Moreover, the assay was reproducible, showing little variation with time in individuals. However, the intra-subject variation on a particular test occasion was high and is difficult to interpret although it has been observed before (Morgan & Crossen, 1977;Crossen et al, 1977).…”

Section: Discussionmentioning

confidence: 89%

“…The mean rate of SCE in cultured lymphocytes from the general population as reported by other groups varies widely, between 5 and 14 per cell (Galloway & Evans, 1975;Dauod et al, 1976;Crossen et al, 1977;Raposa, 1978). It is not clear how much of this variation is due to real differences in the various populations studied, but it is likely that much of it is artefactual, reflecting relatively small sample numbers and differences in culture and staining techniques.…”

Section: Discussionmentioning

confidence: 94%

Sister chromatid exchange in lymphocytes from renal transplant recipients with and without cancer

Kelly¹,

Sheil²

1983

Br J Cancer

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Sister chromatid exchange and micronucleus frequency in human lymphocytes of 1,650 subjects in an Italian population: II. Contribution of sex, age, and lifestyle

Barale

Chelotti

Davini

et al. 1998

Environ. Mol. Mutagen.

125

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Sister chromatid exchange (SCE) and micronuclei (MN) analysis was carried out on 1,650 healthy individuals living in Pisa and in two nearby small cities, Cascina and Navacchio (Ca‐Na). The effect of smoking on SCEs was linearly correlated with the number of cigarettes per day, and an increase of 7.3% SCEs was detectable for as few cigarettes as 1–10/day. Ex‐smokers showed intermediate mean values of SCEs (8.09 ± 1.88) in comparison with never smokers (7.54 ± 1.61) and current smokers (8.45 ± 1.94). Mean values of SCEs of ex‐smokers decreased linearly with time of smoking cessation, reaching the mean values of never smokers within 8 years. The extent of SCE decrease was inversely proportional to the number of cigarettes previously smoked. No interaction between smoking habits and coffee or alcohol drinking on SCEs was observed. A borderline (P = 0.053) increase in mean SCE values in coffee drinkers (more than 3 cups/day) was found. The age effect on SCEs was remarkable in Ca‐Na, but not in Pisa donors. Job type was not associated with significant modification of mean values of SCEs. Multiple logistic regression analysis revealed a statistically significant association between the proportion of high frequency cells (HCF) outliers and coffee consumption. Age and sex appeared to be by far the most important variables associated with modifications in MN frequency, which increased by 0.04 and 0.02 per year in males and females, respectively. Children and young donors (age ≤ 40 years) showed lower MN frequency regardless of sex, whereas sex appeared to determine a significantly higher increase of MN only in females older than 40 years. In contrast, in males the MN rate by age tended to level off after the age of 30–50. MN frequencies of Pisa blue‐ and white‐collar workers were statistically significantly higher than in students (+0.71 and +0.55, respectively). Smoking did not determine any increase of MN frequency. A total lack of correlation (P = 0.913) between MN and SCEs was observed. Environ. Mol. Mutagen. 31:228–242, 1998 © 1998 Wiley‐Liss, Inc.

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The incidence of sister chromatid exchanges in cultured human lymphocytes

Cited by 105 publications

References 20 publications

Chromosomal methods in population studies.

Chromosomal methods in population studies.

Sister chromatid exchange in lymphocytes from renal transplant recipients with and without cancer

Sister chromatid exchange and micronucleus frequency in human lymphocytes of 1,650 subjects in an Italian population: II. Contribution of sex, age, and lifestyle

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