The incorporation of monomethylethanolamine and dimethylethanolamine in fetal brain aggregating cell culture

Dainous, Francine; Kanfer, Julian N.

doi:10.1007/bf00971848

Cited by 5 publications

(2 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Isolated neurons from chick embryos in primary cultures, when incubated in the presence of radioactive Etn, were shown to produce labelled PCho, CDP-Cho and PtdCho [17]. In addition, [3H]MeEtn and [3H]Me2Etn were both converted to their corresponding phosphorylated derivatives and phospholipids by fetal rat brain aggregating cell cultures [18].…”

Section: Introductionmentioning

confidence: 99%

Conversion of ethanolamine, monomethylethanolamine and dimethylethanolamine to choline-containing compounds by neurons in culture and by the rat brain

Andriamampandry

Freysz

Kanfer

et al. 1989

Biochemical Journal

View full text Add to dashboard Cite

The incubation of neurons from chick embryos in primary culture with [3H]ethanolamine revealed the conversion of this base into monomethyl, dimethyl and choline derivatives, including the corresponding free bases. Labelling with [methyl-3H]monomethylethanolamine and [methyl-3H]dimethylethanolamine supported the conclusion that in chick neuron cultures, phosphoethanolamine appears to be the preferential substrate for methylation, rather than ethanolamine or phosphatidylethanolamine. The methylation of the latter two compounds, in particular that of phosphatidylethanolamine, was seemingly stopped at the level of their monomethyl derivatives. Fetal rat neurons in primary culture incubated with [3H]ethanolamine showed similar results to those observed with chick neurones. However, phosphoethanolamine and phosphatidylethanolamine and, to a lesser extent, free ethanolamine, appeared to be possible substrates for methylation reactions. The methylation of water-soluble ethanolamine compounds de novo was further confirmed by experiments performed in vivo by intraventricular injection of [3H]ethanolamine. Phosphocholine and the monomethyl and dimethyl derivatives of ethanolamine were detected in the brain 15 min after injection.

show abstract

Section: Introductionmentioning

confidence: 99%

Conversion of ethanolamine, monomethylethanolamine and dimethylethanolamine to choline-containing compounds by neurons in culture and by the rat brain

Andriamampandry

Freysz

Kanfer

et al. 1989

Biochemical Journal

View full text Add to dashboard Cite

show abstract

“…The rate of formation of radioactive products was concentration dependant up to 4 mM [ 3 H]DMAE, the highest concentration tested. The authors calculated an apparent half-life of 24 hours (Dainous and Kanfer, 1988).…”

Section: Chemical Disposition Metabolism and Toxicokineticsmentioning

confidence: 99%

Glaucopsia in Emergency Department

Uzunget¹,

Kemal²,

Bilir³

et al. 2019

Int J Crit Care Emerg Med

View full text Add to dashboard Cite

Executive SummaryNomination Dimethylethanolamine (DMAE) was nominated by the NIEHS for toxicological characterization, including metabolism, reproductive and developmental toxicity, subchronic toxicity, carcinogenicity and mechanistic studies. The nomination is based on the potential for widespread human exposure to DMAE through its use in industrial and consumer products and an inadequate toxicological database. Studies to address potential hazards of consumer (e.g. dietary supplement) exposures, including use by pregnant women and children, and the potential for reproductive effects and carcinogenic effects are limited. DMAE and related ethanolamines appear to interfere with choline uptake and utilization and may also generate nitrosamines. Further studies are recommended to address these data gaps with special attention to pharmacokinetics and the influence of dietary choline. Consideration should be given to whether the bitartrate salt of DMAE (a form commonly used in dietary supplements) or other DMAE derivative is more appropriate than the free base for use in toxicology studies. Nontoxicological DataChemical Identification The principal compounds related to DMAE [108-01-0] that have common commercial uses and are discussed in this report, are DMAE aceglumate [3342-61-8], DMAE p-acetamidobenzoate (an ester) [2811-31-6], DMAE p-acetamidobenzoate (a salt); Deaner  [3635-74-3], DMAE bitartrate [5988 51-2], DMAE dihydrogen phosphate [6909-62-2], DMAE hydrochloride [2498-25-1], DMAE orotate [1446-06-6], DMAE succinate; tonibral [10549-59-4], centrophenoxine; dimethylethanolamine p-chlorophenoxyacetate hydrochloride [3685-84-5], centrophenoxine orotate [27166-15-0], and meclofenoxate; DMAE p-chlorophenoxyacetate [51-68-3].

show abstract

Partial purification of two forms of Choline kinase and separation of Choline kinase from sphingosine kinase of rat brain

Cao

Kanfer

1995

Neurochem Res

View full text Add to dashboard Cite

Choline kinase of rat brain was purified approximately 200,000 fold using acid precipitation, ammonium sulphate fractionation, Q-Sepharose, Octyl-Sepharose and AH-Sepharose chromatography. The ability of this enzyme to catalyze the phosphorylation of choline, ethanolamine (Etn), monomethylethanolamine (MeEtn), dimethylethanolamine (Me2Etn) and sphingosine was investigated. Choline kinase was separated from sphingosine kinase. The fraction with highly purified choline kinase had four major polypeptides with different molecular masses and possessed activities towards choline, Etn, MeEtn and Me2Etn. Two forms of choline kinase were obtained when the enzymatically active fractions eluted from the Q-Sepharose column were subjected to a horizontal isoelectrofocusing electrophoresis. One form focused around pH 4.7 and is able to phosphorylate choline, Etn, MeEtn and Me2Etn. The other form focused around pH 10 and possessed only choline kinase activity. The latter form of choline kinase did not display classical Michaelis-Menten's mechanism but revealed a positive co-operative pattern for two choline binding sites. This form was purified to apparent homogeneity with a approximate molecular mass of 14.4 kDa.

show abstract

The incorporation of monomethylethanolamine and dimethylethanolamine in fetal brain aggregating cell culture

Cited by 5 publications

References 26 publications

Conversion of ethanolamine, monomethylethanolamine and dimethylethanolamine to choline-containing compounds by neurons in culture and by the rat brain

Conversion of ethanolamine, monomethylethanolamine and dimethylethanolamine to choline-containing compounds by neurons in culture and by the rat brain

Glaucopsia in Emergency Department

Partial purification of two forms of Choline kinase and separation of Choline kinase from sphingosine kinase of rat brain

Contact Info

Product

Resources

About