The increasing of odontoblast-like cell number on direct pulp capping of Rattus norvegicus using chitosan

Prananingrum, Widyasri

doi:10.20473/j.djmkg.v43.i4.p168-171

Cited by 3 publications

(4 citation statements)

References 5 publications

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“…It has incomplete setting reaction and ionized into calcium and hydroxide that triggered the risk of internal root resorption and apical lesion. 14 19 When compared to CH and Ca(OH) 2 , blood vessel and fibroblast proliferations were the lowest in the control group using glass ionomer. There were chronic inflammation and lack of dentin formation caused by persistent acidity of the glass ionomer.…”

Section: Discussionmentioning

confidence: 94%

“…CH contains active N-acetyl-D-glucosamine dimer, which cross-links with glycosaminoglycan and glycoproteins and activates macrophages to secret growth factors such as VEGF, FGF, TGFβ1, and angiopontin. 3 14 Previous studies reported that loading angiogenic growth factor on biomaterials could enhance pulp tissue healing. VEGF is an angiogenic growth factor that stimulates the formation of new blood vessel.…”

Section: Discussionmentioning

confidence: 99%

“…The occlusal surface of the molar of the R. norvegicus rat was prepared with class I cavity by using low-speed tapered round diamond burr, and then perforated with the tip of the explorer under anesthesia using ketamine 10% injection (Kepro Pharmacy, the Netherlands) at a dose of 0.1 mL/100 g body weight and xylazine 2% injection (Xyla, Interchemie, the Netherlands) at 0.01 mL/100 g body weight intramuscularly in the upper thigh. 13 14 In the KA control group, the cavity was filled with glass ionomer cement and the cavity was filled with Ca(OH) 2 in the KB group. In the treatment groups, the PA group, the cavity was filled with CH; in the PB group, the cavity was filled with HA; and in the PC group, the cavity was filled with CH and HA (CH-HA).…”

Section: Methodsmentioning

confidence: 99%

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Potency of the Combination of Chitosan and Hydroxyapatite on Angiogenesis and Fibroblast Cell Proliferation in Direct Pulp Capping of Rattus norvegicus

Sularsih,

Fransiska,

Salsabila

et al. 2024

Eur J Dent

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Objectives The aim of this research was to analyze expression of vascular endothelial growth factor (VEGF), blood vessels, and fibroblast cell proliferation in direct pulp capping treatment of Rattus norvegicus using a combination of chitosan and hydroxyapatite paste. Materials and Methods The samples were male R. norvegicus strains of Wistar rats, weighing 200 to 250 g and aged between 8 and 16 weeks. The occlusal surface of the molars of R. norvegicus was prepared with class I cavity and then perforated with the tip of an explorer. Sixty male R. Norvegicus rats were divided into the following: control group (KA) in which the cavity was filled with glass ionomer cement; control group (KB), in which the cavity was filled with Ca(OH)2; PA group, in which the cavity was filled with chitosan (CH); PB group, in which the cavity was filled with hydroxyapatite (HA); and PC group, in which the cavity was filled with chitosan and hydroxyapatite (CH-HA). Each group was divided into 3-, 7-, and 14-day observation groups. The mandibular bone of the molar was cut and histopathological examination was performed to analyze the blood vessels and fibroblast cell proliferation. Immunohistochemistry examination was done to examine the expression of VEGF. Statistical Analysis The data variation was analyzed with One Way Analysis of Variance (ANOVA) test and continued with multiple comparison Least Significant (LSD) test to determine the different pairs of group. Results Analysis of variance (ANOVA) showed a significant increase in the expression of VEGF, blood vessels, and fibroblast cell proliferations (p ≤ 0.05;), especially in the PC group compared to the other four groups. The least significant test (LSD) test showed significant differences between the groups on the expression of VEGF, blood vessels, and fibroblast cell proliferations. Conclusion The combination of chitosan and hydroxyapatite could promote healing of direct pulp capping treatment by increasing the expression of VEGF, blood vessel, and fibroblast cell proliferation.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Potency of the Combination of Chitosan and Hydroxyapatite on Angiogenesis and Fibroblast Cell Proliferation in Direct Pulp Capping of Rattus norvegicus

Sularsih,

Fransiska,

Salsabila

et al. 2024

Eur J Dent

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“…Red snapper (Lutjanus Sp) scales have chemical constituents: water, protein, fat, carbohydrates, and calcium [12]. In addition, based on the Fourier-transform infrared spectroscopy (FTIR) test results, fish scales also contain chitin, which has the highest polysaccharide structure after cellulose [13]. Red snapper scales have a percentage degree of chitin deacetylation of 80%, more significant than the scales of other fish.…”

Section: Introductionmentioning

confidence: 99%

Untitled

2023

J. Med. Chem. Sci.

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Pulpitis, or pulp inflammation, is a type of dental disease that is commonly associated with tooth pain symptoms. The immune reaction, characterized by TNF-α biomarkers and leukocyte cells, plays a part in the prevalence of pulpitis. (PMN). The latest innovation in dentistry is nanochitosane, which is derived from red snapper scales and includes calcium and chitin. The nanochitosane found in red snapper fish scales (Lutjanus Sp.) has been shown to decrease tumor necrosis factor (TNF) and pain levels. Twenty-four Sprague Dawley rats were splitted into four groups. Healthy rodents comprised group 1. Rats were used in Group II as a reversible pulpitis model with no material change. Rats were used in Group III as a pulpitis model with calcium hydroxide addition. Group IV included rodents as model pulpitis with red snapper fish scale nanochitosane addition. The inclusion of chitosan therapy reduced leukocyte cells, TNF-levels, and pain significantly in group IV compared to group II without therapy and group III with calcium hydroxide treatment. The results of this study showed that redfish scale nanochitosane can decrease inflammation by lowering TNF-and pain levels.

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The Effect of Chitosan on the Formation of Odontoblast-Like Cells in Reversible Pulpitis (in Vivo Study on Sprague Dawley Rats)

Tifani,

Widyastuti,

Nugrahani

et al. 2023

Advances in Health Sciences Research

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The increasing of odontoblast-like cell number on direct pulp capping of Rattus norvegicus using chitosan

Cited by 3 publications

References 5 publications

Potency of the Combination of Chitosan and Hydroxyapatite on Angiogenesis and Fibroblast Cell Proliferation in Direct Pulp Capping of Rattus norvegicus

Potency of the Combination of Chitosan and Hydroxyapatite on Angiogenesis and Fibroblast Cell Proliferation in Direct Pulp Capping of Rattus norvegicus

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The Effect of Chitosan on the Formation of Odontoblast-Like Cells in Reversible Pulpitis (in Vivo Study on Sprague Dawley Rats)

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