2010
DOI: 10.1186/1471-2121-11-54
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The individual-cell-based cryo-chip for the cryopreservation, manipulation and observation of spatially identifiable cells. I: Methodology

Abstract: BackgroundCryopreservation is the only widely applicable method of storing vital cells for nearly unlimited periods of time. Successful cryopreservation is essential for reproductive medicine, stem cell research, cord blood storage and related biomedical areas. The methods currently used to retrieve a specific cell or a group of individual cells with specific biological properties after cryopreservation are quite complicated and inefficient.ResultsThe present study suggests a new approach in cryopreservation, … Show more

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Cited by 12 publications
(7 citation statements)
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“…Deutsch et al 82 developed an Individual Cell-based Cryo-Chip (i3C) made of mold-injected polycarbonate and BK7 glass. The device featured an array of picowells, each of which was designed to maintain an individual cell in position during cryopreservation and accompanying treatments.…”
Section: Role Of Microfluidics In Cryopreservation Of Gametes and Embmentioning
confidence: 99%
“…Deutsch et al 82 developed an Individual Cell-based Cryo-Chip (i3C) made of mold-injected polycarbonate and BK7 glass. The device featured an array of picowells, each of which was designed to maintain an individual cell in position during cryopreservation and accompanying treatments.…”
Section: Role Of Microfluidics In Cryopreservation Of Gametes and Embmentioning
confidence: 99%
“…Later, Deutsh et al and Afrimzon et al (Afrimzon et al, 2010; Deutsch et al, 2010) introduced an individual cell-based cryo-chip (i3C) with an array of picowells (Figure 8B), enabling individual cell cryopreservation, observation, and retrieval for the first time. Furthermore, i3C is compatible with commonly used, commercially available cryostage (MDBCS 600; Linkam Instruments, Tadworth, UK), which extends its application to include programmed freezing and thawing of cells on a chip.…”
Section: Cooling and Warming: Programmable Freezing And Vitrificationmentioning
confidence: 99%
“…(C) A microfabricated chip with an incubation microchamber, microfluidic channels, and microheaters for on-chip cell cryopreservation. Reproduced from (Afrimzon et al, 2010; Deutsch et al, 2010; Li et al, 2014; Li et al, 2010) with permission.…”
Section: Figurementioning
confidence: 99%
See 1 more Smart Citation
“…We have recently introduced a novel device: the individual-cell-based cryo-chip (i3C) for the cryopreservation, manipulation and observation of spatially identifiable cells [ 11 ]. The aim of the present study is to evaluate the fate of cryopreserved cells in the device and to develop an approach for the efficient detection of viable, specific, highly biologically active cells for future applications.…”
Section: Introductionmentioning
confidence: 99%