“…Due to numerous intrinsic features, such as ease of mass production and specific designs and changes, ease of component integration, disposability, low cost, and requirement of smaller reagents or analyte volumes, as well as ease of rapid implementation of a high-efficiency mixture, microfluidics techniques emerged in early 1980 have been introduced for cryopreservation approaches. These fields include controlled loading/unloading of CPAs into the cells with stepwise, linear and complex CPA profiles (Heo et al, 2011; Park et al, 2011; Song et al, 2009), programmable freeze-thawing cycles (Afrimzon et al, 2010; Deutsch et al, 2010; Li et al, 2010), and low-CPA vitrification by ultra-rapid freezing (Choi et al, 2015a; He et al, 2008; Zou et al, 2013) using microfluidics. Such innovation offers a tremendous potential to revolutionize cryopreservation.…”