The INFLORESCENCE DEFICIENT IN ABSCISSION-LIKE6 Peptide Functions as a Positive Modulator of Leaf Senescence in Arabidopsis thaliana

Abstract

Leaf senescence is a highly coordinated process and has a significant impact on agriculture. Plant peptides are known to act as important cell-to-cell communication signals that are involved in multiple biological processes such as development and stress responses. However, very limited number of peptides has been reported to be associated with leaf senescence. Here, we report the characterization of the INFLORESCENCE DEFICIENT IN ABSCISSION-LIKE6 (IDL6) peptide as a regulator of leaf senescence. The expression of IDL6 was up-regulated in senescing leaves. Exogenous application of synthetic IDL6 peptides accelerated the process of leaf senescence. The idl6 mutant plants showed delayed natural leaf senescence as well as senescence included by darkness, indicating a regulatory role of IDL6 peptides in leaf senescence. The role of IDL6 as a positive regulator of leaf senescence was further supported by the results of overexpression analysis and complementation test. Transcriptome analysis revealed differential expression of phytohormone-responsive genes in idl6 mutant plants. Further analysis indicated that altered expression of IDL6 led to changes in leaf senescence phenotypes induced by ABA and ethylene treatments. The results from this study suggest that the IDL6 peptide positively regulates leaf senescence in Arabidopsis thaliana.

Overexpression of the potato VQ31 enhances salt tolerance in Arabidopsis

“…After centrifugation at 12,000 ×g at 4°C for 30 min, the supernatant was taken, and the protein concentration was determined using the BCA Protein Assay Kit, following the manufacturer's instructions (Stanley et al, 2020). Chlorophyll was determined based on the protocol by Guo et al, with slight modifications (Guo et al, 2022). The measurement of soluble sugars was slightly modified based on the Dubois et al (1951) method, and the content of MDA was determined by the thiobarbituric acid (TBA) method (Basit et al, 2022).…”

“…In terms of protein structure, they possess a core FxxxVQxLTG sequence containing 5 highly conserved amino acids, where x is any amino acid (Shan et al, 2021). Subsequently, VQ genes were discovered in other organisms, such as Arabidopsis, rice, maize, grape, soybean, potato, bamboo, poplar, and tea, with a total of 34, 40, 61, 18, 74, 37, 28, 51, and 25 VQ members identified, respectively (Kim et al, 2013;Wang et al, 2014Wang et al, , 2015Wang et al, , 2015Chu et al, 2016;Song et al, 2016;Wang et al, 2017;Guo et al, 2018). The VQ family has been extensively studied and, in detail and comprehensively, functionally characterized in Arabidopsis.…”

Antagonistic Regulation of Arabidopsis Leaf Senescence by SCOOP10 and SCOOP12 Peptides via MIK2 Receptor-Like Kinase

“…All data including plant phenotypes and gene expression presented in this study were analyzed with Graphpad Prism 9 and at least there biological replicates were included in each experiment. Chlorophyll content, photochemical efficiency of PSII (Fv/Fm), and ion leakage were determined as previously described 12,14,37…”

“…Additionally, the INFLORESCENCE DEFICIENT IN ABSCISSION-LIKE6 (IDL6) peptide has been reported to positively modulate leaf senescence in Arabidopsis thaliana . idl6 mutants display delayed leaf senescence, while overexpression of IDL6 accelerates leaf senescence 14 . Furthermore, the plant elicitor peptide PEP1, one of the first plant phytocytokines identified to regulate plant immune responses 10,15,16 , has also been shown to function as a leaf senescence modulator in Arabidopsis 17 .…”