The Influence of 1BL.1RS Translocation on Technological Quality of Winter Wheat

Milovanovic, M.; Perovic, Dragan; Šarić, M.; Prodanovic, S.; Pavlović, Ksenija; Jestrović, Zorica; Protic, R.

doi:10.1007/bf03543506

Cited by 3 publications

(1 citation statement)

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“…In contrast, introgressions from crop wild relatives are substitutions or additions of large chromosomal regions and have been used to improve crop plants (Zamir, 2001; Dempewolf et al, 2017), e.g., as source of resistance or tolerance to biotic and abiotic stress in wheat (Rabinovich, 1998; Crespo-Herrera et al, 2017). Experimental methods, such as C-banding (Friebe et al, 1996), dot-blot genomic hybridization (Rey and Prieto, 2017), fluorescence in-situ hybridization (FISH) (Rayburn and Gill, 1986; Schneider et al, 2005), genomic in-situ hybridization (GISH) (Le et al, 1989; Schwarzacher et al, 1989) and acid or SDS-PAGE (Milovanović et al, 1998), are the state-of-the-art wet-lab techniques for detection and characterization of introgressions. However, these techniques are sophisticated and can only be handled by few labs.…”

Section: Introductionmentioning

confidence: 99%

Detecting Large Chromosomal Modifications Using Short Read Data From Genotyping-by-Sequencing

et al. 2019

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Markers linked to agronomic traits are of the prerequisite for molecular breeding. Genotyping-by-sequencing (GBS) data enables to detect small polymorphisms including single nucleotide polymorphisms (SNPs) and short insertions or deletions (InDels) that can be used, for instance, for marker-assisted selection, population genetics, and genome-wide association studies (GWAS). Here, we aim at detecting large chromosomal modifications in barley and wheat based on GBS data. These modifications could be duplications, deletions, substitutions including introgressions as well as alterations of DNA methylation. We demonstrate that GBS coverage analysis is capable to detect Hordeum vulgare/Hordeum bulbosum introgression lines. Furthermore, we identify large chromosomal modifications in barley and wheat collections. Hence, large chromosomal modifications, including introgressions and copy number variations (CNV), can be detected easily and can be used as markers in research and breeding without additional wet-lab experiments.

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Section: Introductionmentioning

confidence: 99%

Detecting Large Chromosomal Modifications Using Short Read Data From Genotyping-by-Sequencing

et al. 2019

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Fast identification of wheat 1BL.1RS translocation by reversed-phase ultra-performance liquid chromatography (RP-UPLC)

et al. 2013

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Abstract. The 1BL.1RS chromosomal translocation in wheat is the result of replacement of the short arm of chromosome 1B of wheat by the short arm of chromosome 1R of rye, which had been widely used as a parental line in worldwide wheat breeding, resulting in a high percentage of wheat cultivars containing this translocation. A fast and reliable approach to identify this translocation is highly desirable in modern wheat breeding. This study compared reversed-phase ultraperformance liquid chromatography (RP-UPLC), acidic polyacrylamide gel electrophoresis (A-PAGE), liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), allelic-specific PCR, and reversed-phase highperformance liquid chromatography (RP-HPLC) approaches to identify the 1BL.1RS translocation in 76 bread wheat cultivars. Two gliadin bands in the Gli-B1 region of A-PAGE separation were confirmed by LC-MS/MS to be omega secalins from the 1BL.1RS translocation, and they can be used as reliable protein markers for identifying the translocation. A few specific minor peaks eluted at 12-13 min on the RP-UPLC patterns can readily differentiate the 1BL.1RS translocation. Of the 76 wheat cultivars tested, 40 were identified as carrying the 1BL.1RS translocation by RP-UPLC, which was consistent with the results of A-PAGE, HPLC, and PCR. Compared with other established methods, RP-UPLC showed a clear advantage in fast identification of the 1BL.1RS translocation with higher reliability and lower costs, and it is therefore ideal for large-scale screening of the 1BL.1RS translocation in wheat breeding.

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Wheat Storage Proteins: Glutenin Diversity in Sphaerococcum Mutant Forms of Tetraploid Wheats

Georgiev

Dekova

2005

Biotechnology & Biotechnological Equipment

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The Influence of 1BL.1RS Translocation on Technological Quality of Winter Wheat

Cited by 3 publications

References 2 publications

Detecting Large Chromosomal Modifications Using Short Read Data From Genotyping-by-Sequencing

Detecting Large Chromosomal Modifications Using Short Read Data From Genotyping-by-Sequencing

Fast identification of wheat 1BL.1RS translocation by reversed-phase ultra-performance liquid chromatography (RP-UPLC)

Wheat Storage Proteins: Glutenin Diversity in Sphaerococcum Mutant Forms of Tetraploid Wheats

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