The influence of alkaloids on oxidative stress and related signaling pathways

Macáková, Kateřina; Afonso, Rita; Saso, Luciano; Mladěnka, Přemysl

doi:10.1016/j.freeradbiomed.2019.01.026

Cited by 87 publications

(52 citation statements)

References 186 publications

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“…11 Oxidative Medicine and Cellular Longevity wide range of biological effects, including antioxidant and anti-inflammatory effects and Aβ production-and clearance-regulated effects [40][41][42]. In the present study, we confirmed that palmatine, a naturally occurring isoquinoline alkaloid, significantly suppressed Aβ-induced paralysis and displayed neuroprotective effects in the Aβ-transgenic CL2006 and CL4176 strains.…”

Section: Discussionsupporting

confidence: 78%

Neuroprotective Effects of Palmatine via the Enhancement of Antioxidant Defense and Small Heat Shock Protein Expression in Aβ‐Transgenic Caenorhabditis elegans

Jia

Cheng

et al. 2021

Oxidative Medicine and Cellular Longevity

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Palmatine is a naturally occurring isoquinoline alkaloid that has been reported to display neuroprotective effects against amyloid-β- (Aβ-) induced neurotoxicity. However, the mechanisms underlying the neuroprotective activities of palmatine remain poorly characterized in vivo. We employed transgenic Caenorhabditis elegans models containing human Aβ1-42 to investigate the effects and possible mechanisms of palmatine-mediated neuroprotection. Treatment with palmatine significantly delayed the paralytic process and reduced the elevated reactive oxygen species levels in Aβ-transgenic C. elegans. In addition, it increased oxidative stress resistance without affecting the lifespan of wild-type C. elegans. Pathway analysis suggested that the differentially expressed genes were related mainly to aging, detoxification, and lipid metabolism. Real-time PCR indicated that resistance-related genes such as sod-3 and shsp were significantly upregulated, while the lipid metabolism-related gene fat-5 was downregulated. Further studies demonstrated that the inhibitory effects of palmatine on Aβ toxicity were attributable to the free radical-scavenging capacity and that the upregulated expression of resistance-related genes, especially shsp, whose expression was regulated by HSF-1, played crucial roles in protecting cells from Aβ-induced toxicity. The research showed that there were significantly fewer Aβ deposits in transgenic CL2006 nematodes treated with palmatine than in control nematodes. In addition, our study found that Aβ-induced toxicity was accompanied by dysregulation of lipid metabolism, leading to excessive fat accumulation in Aβ-transgenic CL4176 nematodes. The alleviation of lipid disorder by palmatine should be attributed not only to the reduction in fat synthesis but also to the inhibition of Aβ aggregation and toxicity, which jointly maintained metabolic homeostasis. This study provides new insights into the in vivo neuroprotective effects of palmatine against Aβ aggregation and toxicity and provides valuable targets for the prevention and treatment of AD.

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Section: Discussionsupporting

confidence: 78%

Neuroprotective Effects of Palmatine via the Enhancement of Antioxidant Defense and Small Heat Shock Protein Expression in Aβ‐Transgenic Caenorhabditis elegans

Jia

Cheng

et al. 2021

Oxidative Medicine and Cellular Longevity

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“…Induction of MAPK cascade transcriptionally elicits alkaloids biosynthesis to reduce ROS accumulation through their inhibitory effect on NADPH-oxidase, the key enzyme for ROS production [49]. Thus, our results reflected positive correlations between each of AgNPs concentration, H 2 O 2 content, and expression of CrMPK3 gene on one side and expression of STR gene as well as alkaloids content on the other side.…”

Section: Discussionsupporting

confidence: 56%

Boosting of Antioxidants and Alkaloids in Catharanthus roseus Suspension Cultures Using Silver Nanoparticles with Expression of CrMPK3 and STR Genes

Fouad

Hegazy

Azab

et al. 2021

Plants

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Global agricultural systems are under unprecedented pressures due to climate change. Advanced nano-engineering can help increase crop yields while ensuring sustainability. Nanotechnology improves agricultural productivity by boosting input efficiency and reducing waste. Alkaloids as one of the numerous secondary metabolites that serve variety of cellular functions essential for physiological processes. This study tests the competence of silver nanoparticles (AgNPs) in boosting alkaloids accumulation in Catharanthus roseus suspension cultures in relation to the expression of C. roseus Mitogen Activated Protein Kinase 3 (CrMPK3) and Strictosidine Synthase (STR) genes. Five concentrations (5, 10, 15, 20 and 25 mg·L−1) of AgNPs were utilized in addition to deionized water as control. Results reflected binary positive correlations among AgNPs concentration, oxidative stress indicated with increase in hydrogen peroxide and malondialdehyde contents, activities of ascorbate peroxidase and superoxide dismutase, expression of the regulatory gene CrMPK3 and the alkaloid biosynthetic gene STR as well as alkaloids accumulation. These correlations add to the growing evidence that AgNPs can trigger the accumulation of alkaloids in plant cells through a signaling pathway that involves hydrogen peroxide and MAPKs, leading to up-regulation of the biosynthetic genes, including STR gene.

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“…Also, palmitic and oleic acids have antioxidant properties, 21 while 5,8,11,14-eicosatetraenoic acid (ETYA) has the ability to inhibit lipoxigenase activity as a trigger of inflammation, and is associated with the inhibition of adrenocorticotropic release (ACTH). 22 In addition, alkaloids are able to inhibit oxidative damage to tissue and restore endogenous antioxidant enzyme activity, 23 while steroids, phenols, and tannins, have antioxidant properties. 24…”

Section: Cortisolmentioning

confidence: 99%

Anti-stress Effect of Brown Seaweed Sargassum hystrix J. Agard Ethanol Extract on Cold-Induced Stress in Wistar Rats

2021

TJNPR

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According to Ravindran et al., 1 the phenomenon of stress is commonly experienced by humans and is now a common occurrence in modern life. Meanwhile, the condition of oxidative stress is characterized by more free radicals, compared to antioxidants. 2 In cases where free radical production is higher than the rate of neutralization by intracellular antioxidants, the surplus free radicals are potential causes of damage to cells. This is commonly called oxidative damage, the destruction of cell biomolecules due to reaction with free radicals. 3 A study by Khotari et al. 4 reported these free radicals to be alleged causes of apoptosis, lipid peroxidation in cell membrane, and damage to DNA (deoxyribo nucleic acid), while a report by Manisha et al. 2 showed this tends to have consequent impacts on the body, including cancer and other serious ailments. Marine algae is a significant traditional food in Japan, Korea, and other eastern countries. 5 Meanwhile, the brown algae Sargassum sp. is common to the southern beaches of Gunungkidul, in Yogyakarta, Indonesia. This alga contains bioactive compounds with antitumour, antioxidant, antifungal, antihypertensive, antiviral, and antidiabetic properties. 6-10 A study by Lailatussifa et al. 11 reported Sargassum polycystum to contain polyphenols which is able to inhibit oxidative stress due to cold, while Budhiyanti et al. 12 stated S. hystrix possesses polyphenols with antioxidant properties. Therefore, marine algae are able to reduce oxidative stress. Bali and Jaggi 13 reported numerous pre-clinical models for inducing stress, including restraint stress in animals, while Di-Cerbo et al. 14 reported cold restraint stress to be widely used in evaluating the antistress effects exhibited by the extracts of Borago officinalis, S. polycystum extract 11 and Eugenia caryophyllys buds. 15

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The influence of alkaloids on oxidative stress and related signaling pathways

Cited by 87 publications

References 186 publications

Neuroprotective Effects of Palmatine via the Enhancement of Antioxidant Defense and Small Heat Shock Protein Expression in Aβ‐Transgenic Caenorhabditis elegans

Neuroprotective Effects of Palmatine via the Enhancement of Antioxidant Defense and Small Heat Shock Protein Expression in Aβ‐Transgenic Caenorhabditis elegans

Boosting of Antioxidants and Alkaloids in Catharanthus roseus Suspension Cultures Using Silver Nanoparticles with Expression of CrMPK3 and STR Genes

Anti-stress Effect of Brown Seaweed Sargassum hystrix J. Agard Ethanol Extract on Cold-Induced Stress in Wistar Rats

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