The Influence of Auxins on the Biosynthesis of Isoprene Derivatives in Callus Cultures of Vaccinium corymbosum var. bluecrop

Abstract

Callus cultures of Vaccinium corymbosum var. bluecrop were optimized for their isoprene derivatives production by supplementing Schenk-Hildebrandt (SH) medium with constant concentration of kinetin (2.32 μm) and two different amounts of selected auxins. Every auxin, except for IBA, used in 10-time higher concentration (2,4D, NAA, IAA, NOA) stimulated biosynthesis of -sitosterol and inhibited triterpene synthesis. Quantitative analysis of isoprene derivatives in callus biomass collected on the 25 th day of the experiment proved that the analyzed callus of Vaccinium corymbosum var. bluecrop synthesized the highest amount of isoprene derivatives after subculturing on SH medium modified with 22.6 μm of 2,4D and 2.32 μm of kinetin.

ESTIMATION OF SOME SECONDARY METABOLITES FROM THE IN VITRO CULTURES OF CHLOROPHYTUM BORIVILIANUM SANT. Et. FERN

“…Beta-sitosterol has been isolated from the cultured tissues of Terminalia chebula [31] as well as the callus of several plants viz. Morus alba [32], Vaccinium corymbosum [33], Cissus quadrangularis [34]. Bathoju and Giri [35] (2012) also reported the production of two important sapogenins; stimasterol and hecogenin from the in vitro regenerated root cultures of C. borivilianum.…”

Endophytic Bacillus megaterium and exogenous stimuli affect the quinonemethide triterpenes production in adventitious roots of Peritassa campestris (Celastraceae)

“…Given this diversity of effects, it is difficult to standardize responses related to the effects of auxins on different species, including their influences on secondary metabolism. However, some auxins can stimulate triterpene biosynthesis (Migas et al 2006;Singh et al 2015;Rasool and Mohamed 2016). Smolenskaya et al (2007) found that 2,4-D induced mitosis in Panax ginseng cells, which resulted in a very low accumulation of ginsenosides (steroid glycosides and triterpene saponins), whereas the NAA induced low cell mitosis and promoted cellular elongation, nuclear DNA synthesis, and the biosynthesis of the same and different ginsenosides.…”

Genome organization, functional analysis of biosynthetic genes and metabolic diversity of triterpenes in legumes

“…A modified callus growing medium, supplemented with growth regulators of the auxin group, resulted in the production of a triterpene-rich callus culture and β-sitosterol accumulation. β-sitosterol accumulated in amounts which were approximately 10-times higher than for triterpene acids (oleanolic and ursolic acid) and approximately 6-times higher than for amyrines (α-and β-amyrin) (Migas et al, 2006). Auxin and cytokinin have not been elsewhere reported to have functions in inducing triterpene production.…”

“…On the contrary, the exogenous application of cytokinins in C. roseus cells in a 2,4-D-free medium greatly enhanced the accumulation of the indole alkaloid ajmalicine (Yahia et al, 1998). The influence of auxins on isoprene derivatives was investigated in callus cultures of Vaccinium corymbosum var bluecorp (Migas et al, 2006).…”

“…In callus cultures Vaccinium corymbosum var. bluecrop every auxin utilized such as 2,4-D, NAA, indole-3-acetic acid (IAA) and naphthoxyacetic acid (NOA), resulted in the production of a triterpene-rich callus culture and β-sitosterol accumulation (Migas et al, 2006). Generally, auxin and cytokinin have not been reported to have functions in inducing triterpene production.…”