The influence of biofilm maturation on fluoride’s anticaries efficacy

Ayoub, Hadeel M.; Gregory, Richard L.; Tang, Qing; Lippert, Frank

doi:10.1007/s00784-021-04100-6

Cited by 7 publications

(8 citation statements)

References 42 publications

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“…SnF 2 and AmF might be options to treat existing biofilms and inhibit the production of new ones ( Ayoub et al, 2022 ). Another method is to raise the pH in the oral environment using particular ingredients in toothpaste, which can also play a role in preventing caries.…”

Section: Development Of Preventive Measuresmentioning

confidence: 99%

Association of polymicrobial interactions with dental caries development and prevention

et al. 2023

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Dental caries is a common oral disease. In many cases, disruption of the ecological balance of the oral cavity can result in the occurrence of dental caries. There are many cariogenic microbiota and factors, and their identification allows us to take corresponding prevention and control measures. With the development of microbiology, the caries-causing bacteria have evolved from the traditional single Streptococcus mutans to the discovery of oral symbiotic bacteria. Thus it is necessary to systematically organized the association of polymicrobial interactions with dental caries development. In terms of ecology, caries occurs due to an ecological imbalance of the microbiota, caused by the growth and reproduction of cariogenic microbiota due to external factors or the disruption of homeostasis by one’s own factors. To reduce the occurrence of dental caries effectively, and considering the latest scientific viewpoints, caries may be viewed from the perspective of ecology, and preventive measures can be taken; hence, this article systematically summarizes the prevention and treatment of dental caries from the aspects of ecological perspectives, in particular the ecological biofilm formation, bacterial quorum sensing, the main cariogenic microbiota, and preventive measures.

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Section: Development Of Preventive Measuresmentioning

confidence: 99%

Association of polymicrobial interactions with dental caries development and prevention

et al. 2023

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“…The toothpaste formulations have changed considerably to become stable, using reliable vehicles for F delivery [32]. Evidence has demonstrated that modern F toothpastes, including among others either NaF or SnF 2 based toothpastes, are effective at controlling dental caries [33] because of uoride's activity in reducing demineralization and enhancing remineralization. In relation to reducing dental bio lm and affecting your architecture previous studies have shown that SnF 2 -containing toothpastes were able to signi cantly reduce dental bio lm and affect your architecture [32,33].…”

Section: Discussionmentioning

confidence: 99%

“…In relation to reducing dental bio lm and affecting your architecture previous studies have shown that SnF 2 -containing toothpastes were able to signi cantly reduce dental bio lm and affect your architecture [32,33]. This antibacterial effect of SnF 2 can be attributed to the stannous ion [34,35] through its ability to reduce bacterial acidogenicity and glucan production [33]. Moreover, NaF has limited action [32,33].…”

Section: Discussionmentioning

confidence: 99%

“…This antibacterial effect of SnF 2 can be attributed to the stannous ion [34,35] through its ability to reduce bacterial acidogenicity and glucan production [33]. Moreover, NaF has limited action [32,33]. he ineffectiveness of F in inhibiting bio lm growth may be explained by the fact that above pH 5.5 F is found in its dissociated form (F − ) and therefore not able to penetrate the bacterial cell wall.…”

Section: Discussionmentioning

confidence: 99%

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In situ evaluation of a new toothpaste formulation containing fluoride, casein phosphopeptide-amorphous calcium phosphate, and sodium trimetaphosphate on dental enamel demineralization and biofilm variables

Gonçalves,

de Almeida,

Hannig

et al. 2024

Preprint

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Objectives This in situ study aimed to evaluate a new toothpaste formulation containing fluoride (F), casein phosphopeptide amorphous calcium phosphate (CPP-ACP) and sodium trimetaphosphate (TMP) on the process of dental demineralization and biofilm composition. Materials and Methods This crossover double-blind study consisted of five phases, in which 10 volunteers wore intraoral appliances containing four bovine enamel specimens. The cariogenic challenge was performed using 30% sucrose solution. Blocks were treated 3x/day with the following toothpastes: 1) Placebo (No F-TMP-CPP-ACP), 2) 1100 ppm F (1100F), 3) 1100F + 3%TMP (1100F-TMP), 4) 1100F + 10%CPP-ACP (1100F-CPP-ACP) and 5) 1100F-CPP-ACP-TMP. After 7 days, the percentage loss of surface hardness (%SH), integrated loss of subsurface hardness (ΔKHN), F, calcium (Ca) and phosphorus (P) concentration in the enamel was determined. The concentration of F, Ca, P and insoluble extracellular polysaccharide (EPS) in the biofilm were analyzed. Results The addition of CPP-ACP-TMP to 1100F reduced %SH by 42% and 39% when compared to the 1100F and 1100F-CPP-ACP (p < 0.001); in addition, to a reduction in lesion body (ΔKHN) by 36% for the same treatments. The treatment with 1100F-CPP-ACP-TMP led to a significant increase in the concentration of F, P and Ca in the enamel and biofilm, and reduced the concentration of EPS (p < 0.001). Conclusions Toothpaste formulation containing 1100F-CPP-ACP-TMP provided a greater protective effect against tooth enamel demineralization and significantly influenced the composition of the in situ biofilm. Clinical Relevance: These results are promising and provide valuable insights for the design of further clinical trials.

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“…Extremophiles such as fluoride-resistant strain have survival strategies to adjust themselves to suit an extreme environment ( Raddadi et al., 2015 ). It has been reported that biofilm’s maturation can affect the anticaries efficacy of fluoride including LDH activity ( Ayoub et al., 2022 ). We speculate that in FR groups, the priority is to restore primary metabolic disequilibrium instead of establishing a new one under conditions of fluoride stress, so that it can enhance fluoride tolerance.…”

Section: Discussionmentioning

confidence: 99%

Ecological influence by colonization of fluoride-resistant Streptococcus mutans in oral biofilm

Shen

Yu²,

Qiu³

et al. 2023

Front. Cell. Infect. Microbiol.

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BackgroundDental caries is one of the oldest and most common infections in humans. Improved oral hygiene practices and the presence of fluoride in dentifrices and mouth rinses have greatly reduced the prevalence of dental caries. However, increased fluoride resistance in microbial communities is concerning. Here, we studied the effect of fluoride-resistant Streptococcus mutans (S. mutans) on oral microbial ecology and compare it with wild-type S. mutans in vitro.MethodsBiofilm was evaluated for its polysaccharide content, scanning electron microscopy (SEM) imaging, acid-producing ability, and related lactic dehydrogenase (LDH), arginine deiminase (ADS), and urease enzymatic activity determination. Fluorescence in situ hybridization (FISH) and quantitative real-time polymerase chain reaction (qRT-PCR) were used to evaluate the S. mutans ratio within the biofilm. It was followed by 16S rRNA sequencing to define the oral microbial community.ResultsFluoride-resistant S. mutans produced increased polysaccharides in presence of NaF (P < 0.05). The enzymatic activities related to both acid and base generation were less affected by the fluoride. In presence of 275 ppm NaF, the pH in the fluoride-resistant strain sample was lower than the wild type. We observed that with the biofilm development and accumulative fluoride concentration, the fluoride-resistant strain had positive relationships with other bacteria within the oral microbial community, which enhanced its colonization and survival. Compared to the wild type, fluoride-resistant strain significantly increased the diversity and difference of oral microbial community at the initial stage of biofilm formation (4 and 24 h) and at a low fluoride environment (0 and 275 ppm NaF) (P < 0.05). Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that fluoride-resistant strain enhanced the metabolic pathways and glucose transfer.ConclusionsFluoride-resistant S. mutans affected the microecological balance of oral biofilm and its cariogenic properties in vitro, indicating its negative impact on fluoride's caries prevention effect.

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The influence of biofilm maturation on fluoride’s anticaries efficacy

Abstract: and Mr. George Eckert (IU School of Medicine) for their feedback in designing the study and reviewing the results.

Cited by 7 publications

References 42 publications

Association of polymicrobial interactions with dental caries development and prevention

Association of polymicrobial interactions with dental caries development and prevention

In situ evaluation of a new toothpaste formulation containing fluoride, casein phosphopeptide-amorphous calcium phosphate, and sodium trimetaphosphate on dental enamel demineralization and biofilm variables

Ecological influence by colonization of fluoride-resistant Streptococcus mutans in oral biofilm

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