The relevance of the article lies in the determination of the most optimal varieties and biopreparations that ensure high yield and profitability of growing winter barley. The purpose of the research was to determine the main indicators of the economic efficiency of the elements of the technology of growing winter barley for foliar fertilizing of plants in the main periods of vegetation with biological preparations. When conducting research, generally accepted methods were used: systematic approach and systematic analysis, field and statistical. The article provides data on the economic efficiency of the elements of winter barley cultivation technology based on the results of research conducted with four varieties of the crop in the conditions of the Educational and Scientific Practical Center of the Mykolaiv National Agrarian University in 2016-2019. The impact of variety selection, foliar fertilizing with modern bacterial preparations on the main indicators of the economic efficiency of growing winter barley was analysed. It was established that the cost of the grown grain depended on and changed under the influence of the features of the variety, biological preparation and the number of fertilizing. Obtaining the maximum cost of grain ensured the cultivation of winter barley varieties Valkyrie and Oscar for carrying out foliar feeding in the phase of tillering and emergence of plants in the tube with the biological preparation Organic Balance: 26.5 and 26.3 thousand hryvnias/ha, respectively. The same varieties, in case of two-time fertilization with Azotophyt, formed the value of grain at 25 thousand UAH/ha, and in controls – 20.1 and 21.1 thousand UAH/ha, respectively. Indicators of conditional net profit and level of profitability also changed with a similar dependence. Values of the cost of growing a unit of production of winter barley had the opposite dependence. The obtained scientific research results and definitions will contribute to the cultivation of winter barley on the basis of resource conservation with the use of biological preparations to increase productivity and the main factors of economic efficiency