The influence of contamination of culture medium with hepatitis B virus on the outcome of in vitro fertilization pregnancies

Os, H.C. van; Drogendijk, A. C.; Fetter, W. P. F.; Heijtink, R. A.; Zeilmaker, G. H.

doi:10.1016/0002-9378(91)90244-l

Cited by 42 publications

(14 citation statements)

References 19 publications

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“…At the end of 1987, 79 patients became infected with hepatitis B virus (HBV) during an in vitro fertilization procedure due to a contaminated pool of human serum used in embryo culture; 3 of these patients developed chronic hepatitis B infection [Van Os et al, 1991]. Apart from these three patients, we studied all patients (n ‫ס‬ 12) with at least two consecutive HBsAg-, anti-HBc-, and HBeAg-positive samples at first screening in March 1988.…”

Section: Materials and Methods Patientsmentioning

confidence: 99%

Characteristics of the early phase of chronicity in acute hepatitis B infection

Heijtink

Paulij²,

Roosmalen³

et al. 1999

J. Med. Virol.

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The mechanism of development of chronicity after acute hepatitis B infection has not been elucidated fully. Following a single source outbreak of hepatitis B among 79 adult women, three patients (4%) became chronic carriers of hepatitis B virus (HBV). We compared features of the virus and antibody response of the latter three patients with those of 12 HBeAg-positive cases with resolving infection. The virus genotype was D, antigenic subtype ayw2. Base sequence analysis of S- and C-gene regions revealed no differences between the two groups. During the acute illness the three patients who developed chronicity had a remarkable transient reduction of HBsAg, HBeAg, and HBV DNA levels at 14-20 weeks after infection, the time of HBeAg seroconversion in the patients who cleared the infection. One HBeAg-specific monoclonal antibody (HBOT.95A) used as solid-phase antibody in a sandwich enzyme immunoassay detected an increased HBeAg signal in 2 of the 3 patients that developed chronicity and in 1 of the 12 patients who recovered. The latter patient had an exceptional long period of HBsAg antigenemia. Standard HBeAg assays detected HBeAg in all cases. HBeAg and anti-HBe-positive serum samples from the patients who recovered could inhibit the HBOT.95A response. The results suggest that chronic hepatitis B develops after an interruption of immune clearance. Differentiation of the antibody response to HBeAg may help to find patients with an increased risk for this interrupted immune clearance who might be candidates for an early intervention therapy.

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Section: Materials and Methods Patientsmentioning

confidence: 99%

Characteristics of the early phase of chronicity in acute hepatitis B infection

Heijtink

Paulij²,

Roosmalen³

et al. 1999

J. Med. Virol.

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“…These workers found that when using HA, the implanted organ structure was visually better than that obtained using silicone gel and saline implants, Moreover there were no reported in vivo side-effects 1 year after the implantation. 68 Kemmann, 1998, 69 Suchanek et al, 1994, 70 Joly et al, 1992, 71 Gardner, 2003, 72 Hyaluronic acid in topical drug delivery…”

Section: Dermatological Applicationsmentioning

confidence: 99%

Hyaluronic acid: a unique topical vehicle for the localized delivery of drugs to the skin

Brown

Jones

2005

Acad Dermatol Venereol

323

221

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Hyaluronic acid (HA) is a naturally occurring polyanionic, polysaccharide that consists of N-acetyl-D-glucosamine and beta-glucoronic acid. It is present in the intercellular matrix of most vertebrate connective tissues especially skin where it has a protective, structure stabilizing and shock-absorbing role. The unique viscoelastic nature of HA along with its biocompatibility and non-immunogenicity has led to its use in a number of clinical applications, which include: the supplementation of joint fluid in arthritis; as a surgical aid in eye surgery; and to facilitate the healing and regeneration of surgical wounds. More recently, HA has been investigated as a drug delivery agent for various routes of administration, including ophthalmic, nasal, pulmonary, parenteral and topical. In fact, regulatory approval in the USA, Canada and Europe was granted recently for 3% diclofenac in 2.5% HA gel, Solaraze, for the topical treatment of actinic keratoses, which is the third most common skin complaint in the USA. The gel is well tolerated, safe and efficacious and provides an attractive, cost-effective alternative to cryoablation, curettage or dermabrasion, or treatment with 5-fluorouracil. The purpose of this review is to describe briefly the physical, chemical and biological properties of HA together with some details of its medical and pharmaceutical uses with emphasis on this more recent topical application.

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“…However, human serum albumin (HSA) is not a stable substance because its biological composition may vary among sources and batches, depending on the degree to which different molecules remain bound to albumin during its preparation (1,4,5). Moreover, the use of blood-derived additives in human in vitro embryo culture systems raises concerns regarding safety owing to the potential risk for transmission of viral contaminants such as hepatitis B and human immunodeficiency virus (6).…”

mentioning

confidence: 99%