The influence of cross-sectional morphology on the compressive resistance of polymeric nerve conduits

Yin, Jun; Zhang, Deming; Xiang, Yang; Wei, Peng; Yang, Zhenyu; Wang, Zonghuan; Fu, Jianzhong

doi:10.1016/j.polymer.2018.06.022

Cited by 19 publications

(11 citation statements)

References 26 publications

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“…Synthetic material-based single-layered nerve conduits, i.e. polyurethane (PU) [ 9 , 29 ], polyacrylonitrile (PAN) [ 6 ], poly ( D, l -lactide-co-glycolide) (PLGA) [ 38 ] have optimized and tunable mechanical performance, but they have poor biocompatibility and biodegradability. Therefore, our strategy to fulfill two contradictory needs was to fabricate a bilayered nerve conduit with a mechanically strong outer layer and biological inner layer.…”

Section: Discussionmentioning

confidence: 99%

“…The mechanical properties of nerve conduits (both single-layered and bilayered) were measured by an ElectroForce (TA Instruments, USA) at room temperature. The compressive modulus of nerve conduits was measured by lateral compression at a rate of 1 mm/min [ 9 , 29 ]. The compressive modulus was defined as:

where F was the compressive force per unit length and ε = ( OD − OD ′)/ OD is the compressive strain.…”

Section: Methodsmentioning

confidence: 99%

“…These features allow the nerve conduit to serve as a substrate that supports appropriate cellular behaviors of regenerating nerves. Second, the nerve conduit should be sufficiently physically robust to resist in vivo physiological invasion, avoid channel collapse, and mechanically match the nerve tissues [ 9 ]. Although, various natural and synthetic materials have been utilized to develop the nerve conduits, the nerve injury repair efficiency of existing nerve conduits is still unsatisfactory due to their inappropriate biological and mechanical properties or lack of structural integration.…”

Section: Introductionmentioning

confidence: 99%

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Additive-lathe 3D bioprinting of bilayered nerve conduits incorporated with supportive cells

Liu

Zhang

et al. 2021

Bioactive Materials

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Nerve conduits have been identified as one of the most promising treatments for peripheral nerve injuries, yet it remains unsolved how to develop ideal nerve conduits with both appropriate biological and mechanical properties. Existing nerve conduits must make trade-offs between mechanical strength and biocompatibility. Here, we propose a multi-nozzle additive-lathe 3D bioprinting technology to fabricate a bilayered nerve conduit. The materials for printing consisted of gelatin methacrylate (GelMA)-based inner layer, which was cellularized with bone marrow mesenchymal stem cells (BMSCs) and GelMA/poly(ethylene glycol) diacrylate (PEGDA)-based outer layer. The high viability and extensive morphological spreading of BMSCs encapsulated in the inner layer was achieved by adjusting the degree of methacryloyl substitution and the concentration of GelMA. Strong mechanical performance of the outer layer was obtained by the addition of PEGDA. The performance of the bilayered nerve conduits was assessed using in vitro culture of PC12 cells. The cell density of PC12 cells attached to cellularized bilayered nerve conduits was more than 4 times of that on acellular bilayered nerve conduits. The proliferation rate of PC12 cells attached to cellularized bilayered nerve conduits was over 9 times higher than that on acellular bilayered nerve conduits. These results demonstrate the additive-lathe 3D bioprinting of BMSCs embedded bilayered nerve conduits holds great potential in facilitating peripheral nerve repair.

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Section: Discussionmentioning

confidence: 99%

where F was the compressive force per unit length and ε = ( OD − OD ′)/ OD is the compressive strain.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Additive-lathe 3D bioprinting of bilayered nerve conduits incorporated with supportive cells

Liu

Zhang

et al. 2021

Bioactive Materials

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“…Yield strength of the composite decreased from 63 to 13 MPa as the porosity increased from 24 to 50%. Yin et al [6] show a similar trend with PLGA fibers, a reduction of modulus from 14.7 to 0.37 MPa as the porosity percentage increase from 35 to 70. Additionally, they showed that the modulus not only depends on the percentage of pores but also on the morphology of pores.…”

Section: Introductionmentioning

confidence: 58%

Bioinspired cellular sheath-core electrospun non-woven mesh

et al. 2019

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Fibers are valuable to biomedical applications. Used as sutures or meshes, there is an increased dual need to provide functionality such as drug delivery. Porosity represents a high surface area to volume architecture. Coaxial fibers with porous and non-porous layers offer a new design framework for fiber design that can resolve dual needs of mechanical robustness with transport phenomena. Using preferential solubility of a polymer in supercritical CO 2 , we develop a new architecture using biocompatible polymers based on porous core-sheath fiber fabrication technique. Polycaprolactone was selected as the CO 2 miscible phase and Poly(butyrate adipate terephthalate)(PBAT) as the immiscible phase. The mechanical performance of the fibers was investigated using quasi static and dynamic loading. SEM images indicate no physical detachment of the two polymer surface after CO 2 exposure indicating a successful amalgamation of polymers at the boundary of core and sheath. PCL as a sheath and as a core showed an increase of 650% and 468% in tensile strength compared to pristine PCL and PBAT. Introduction of porosity on the surface of coaxial fiber fPCL(cPBAT) further enhanced the yield strength increases by 40%. Dynamic mechanical analysis was used to analyze the viscoelastic properties of the fibers. The storage and loss modulus for coaxial fibers shows superior modulus throughout the glassy, glass transition and rubbery region as compared to the pristine PCL and PBAT, showing enhancement in both the elastic and viscous response of the material. The results indicate a new approach that is free of volatile organic solvents to manipulate the architecture of the cross-section of the electrospun fiber and tailor mechanical properties to the required application.

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“…Different biodegradable polymers including chitosan (Muheremu et al, 2017), collagen (Yao et al, 2018), fibrin (Wang et al, 2018), poly(ethylene glycol) (PEG)(X. Liu et al, 2016), poly-lactic-co-glycolic acid (PLGA) (Yin et al, 2018), poly-L-lactide acid (PLLA) (Frost et al, 2018), polycaprolactone (PCL) (Lopez et al, 2018), and poly(caprolactone fumarate) (PCLF) (Golafshan, Gharibi, Kharaziha, & Fathi, 2017) have been investigated for the sake of nerve regeneration through the conduits made thereof. Alginate is an anionic polysaccharide extracted from seaweed that is composed of mannuronic acid and guluronic acid units.…”

Section: Introductionmentioning

confidence: 99%

An electroactive alginate hydrogel nanocomposite reinforced by functionalized graphite nanofilaments for neural tissue engineering

Homaeigohar

Tsai

Young

et al. 2019

Carbohydrate Polymers

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To address the need to biodegradable, electroactive conduits accelerating nerve regeneration, here we develop a nanocomposite hydrogel made of alginate reinforced by citric acid functionalized graphite nanofilaments. The green, simple functionalization enhances the nanofillers distribution and their biocompatibility, as verified using mesenchymal stem cells in vitro. The uniformly distributed nanofilaments raise mechanical stability of the nanocomposite hydrogel versus the neat one up to three times. Also, the nanofilaments enable electrical contact and intercellular signaling thereby stimulating their biological activity. In vitro studies proved the biocompatibility of the nanocomposite hydrogel whereon PC12 cells proliferate and spread evidently. In vivo tests also supported applicability of the nanocomposite hydrogel for implantation within body, and the samples showed no adverse reaction and no inflammatory responses after 14 days. Conclusively, the results certify that the developed electroactive nanocomposite hydrogel is able to stimulate nerve generation and could be confidently used as a nerve conduit material.

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The influence of cross-sectional morphology on the compressive resistance of polymeric nerve conduits

Cited by 19 publications

References 26 publications

Additive-lathe 3D bioprinting of bilayered nerve conduits incorporated with supportive cells

Additive-lathe 3D bioprinting of bilayered nerve conduits incorporated with supportive cells

Bioinspired cellular sheath-core electrospun non-woven mesh

An electroactive alginate hydrogel nanocomposite reinforced by functionalized graphite nanofilaments for neural tissue engineering

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