The influence of cryopreservation and quick-freezing on the mechanical properties of tendons

Oswald, I.; Rickert, Markus; Brüggemann, Gert–Peter; Niehoff, Anja; Ulloa, Carlos Alfonso Fonseca; Jahnke, Alexander

doi:10.1016/j.jbiomech.2017.08.018

Cited by 31 publications

(20 citation statements)

References 12 publications

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“…Meanwhile, the cryopreservation and thawing temperature may affect the tendon's properties of allograft. Thawing at room temperature as we did in this study reduced the maximum stress [25], which may cause higher recurrence after surgical reconstruction in allograft group, whereas the autograft group would not be faced with. This factor may also contribute to the high T2* value in allograft.…”

Section: Discussionmentioning

confidence: 82%

Clinical and magnetic resonance imaging assessment of anatomical lateral ankle ligament reconstruction: comparison of tendon allograft and autograft

Tao

et al. 2018

International Orthopaedics (SICOT)

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Section: Discussionmentioning

confidence: 82%

Clinical and magnetic resonance imaging assessment of anatomical lateral ankle ligament reconstruction: comparison of tendon allograft and autograft

Tao

et al. 2018

International Orthopaedics (SICOT)

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“…This allows decoupling tissue acquisition, which usually has to be done shortly after donor death, and material testing. Especially in case of ligaments and aponeuroses, the influence of freezing and thawing processes on the mechanical properties of human tissue has not yet been fully investigated, despite current research in this field [1][2][3][4][5] . There is a large number of different research results in the field of biomechanical characterization of ligaments and tendons, particularly with regard to the influence of donor age and the influence of freezing effects on the mechanical properties [6][7][8][9][10][11] .…”

Section: The Influence Of Different Sample Preparation On Mechanical mentioning

confidence: 99%

The influence of different sample preparation on mechanical properties of human iliotibial tract

Fischer

Kurz

Höch

et al. 2020

Sci Rep

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In the run-up to biomechanical testing, fresh human tissue samples are often frozen in order to inhibit initial decomposition processes and to achieve a temporal independence of tissue acquisition from biomechanical testing. The aim of this study was to compare the mechanical properties of fresh tissue samples of the human iliotibial tract (IT) to fresh-frozen samples taken from the same IT and those modified with different concentrations of Dimethylsulfoxide (DMSO) prior to freezing. All samples were partial plastinated and destructive tensile tests were conducted with a uniaxial tensile test setup. A plastination technique already established in the laboratory was modified to improve the clamping behaviour of the samples. Material failure was caused by a gradual rupture of the load-bearing collagen fibre bundles. Contrary to our expectations, no significant difference was found between the tensile strength of fresh and fresh frozen specimens. The addition of 1 wt% DMSO did not increase the tensile strength compared to fresh-frozen samples; an addition of 10 wt% DMSO even resulted in a decrease. Based on our findings, the use of simple fresh-frozen specimens to determine the tensile strength is viable; however fresh specimens should be used to generate a complete property profile.

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“…In contrast to our findings, cryopreservation of bone allografts is described in the literature between 6 months to 5 years and even longer without impairments on biomechanics 15 , 16 . Cryopreservation is used also as the method of choice in the preservation of meniscal tissue 13 and tendons 14 . However, it should be mentioned when using these tissues, a longer period of ingrowth is needed for integration into the organism.…”

Section: Discussionmentioning

confidence: 99%

“…It is known that alcohol or formalin fixation changes the plastic mechanical properties of bone and therefore the use of fresh-frozen bone specimens is recommended in biomechanical studies investigating failure loads 12 . Cryopreservation is also the method of choice in the preservation of bone, meniscal tissue 13 and tendons 14 for human use, with preservation times of more than 5 years 15 , 16 . Cryopreservation for example is the only method that preserves fresh meniscus architectural specificities 13 .…”

Section: Introductionmentioning

confidence: 99%

Different storage times and their effect on the bending load to failure testing of murine bone tissue

Tiefenboeck

Payr

Bajenov

et al. 2020

Sci Rep

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Cryopreservation is a well-established method for bone storage. However, the ideal timing of mechanical testing after sacrificing the experimental animals is still under discussion and of significant importance to the presentation of accurate results. Therefore, the aim of this study was to investigate and compare different cryopreservation durations to native murine bone and whether there was an influence on mechanical bone testing. For this study the tibias of 57 female C57BL/6 mice—18-weeks of age—were harvested and randomly allocated to one of four groups with varying storage times: (1) frozen at −80 °C for 3 months, (2) frozen at −80 °C for 6 months, (3) frozen at −80 °C for 12 months and (4) native group. The native group was immediately tested after harvesting. The comparison of the mean strength and load to failure rates demonstrated a significant difference between the storage groups compared to the native control (p = 0.007). However, there was no difference in the strength and the load to failure values of bones of all storage groups when compared against each other. Once cryopreservation at −80 °C is performed, no differences of mechanical bone properties are seen up to 12 months of storage. When actual in vivo data is of close interest, immediate testing should be considered and is preferred. If comparison of groups is required and long-time storage is necessary, cryopreservation seems to be an accurate method at present.

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The influence of cryopreservation and quick-freezing on the mechanical properties of tendons

Cited by 31 publications

References 12 publications

Clinical and magnetic resonance imaging assessment of anatomical lateral ankle ligament reconstruction: comparison of tendon allograft and autograft

Clinical and magnetic resonance imaging assessment of anatomical lateral ankle ligament reconstruction: comparison of tendon allograft and autograft

The influence of different sample preparation on mechanical properties of human iliotibial tract

Different storage times and their effect on the bending load to failure testing of murine bone tissue

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