The Influence of Cryopreservation on Parameters of Energetic Metabolism and Motility of Fowl Spermatozoa

Ochkur, Sergei I.; Kopeika, Evgeniy F.; Suraj, Petr F.; Грищенко, В. И.

doi:10.1006/cryo.1994.1029

Cited by 6 publications

(7 citation statements)

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“…Some parameters which have been used to assess the function or status of frozenthawed chicken spermatozoa in vitro are shown in Table 1. For each parameter, the examples have been selected only on the basis that they cover three decades, different laboratories and different systems for both measurement of the individual parameters Bakst and Sexton, 1979;Westfall and Harris, 1975;Wishart and Palmer, 1986;Ochkur, 1990;Seigneurin and Blesbois, 1995Hypo-osmotic swelling test 62 Gill et al, 1996Oxygen utilization rate 48 Scott et al, 1980ATP levels 26 Wishart and Palmer, 1986 Dye reduction potential 31 Binding to IPVL proteins in vitro 18 Cramer et al, 1994Penetration of IPVL in vitro 8.5 Robertson et al, 1997 and for storage of spermatozoa. Despite this, the percentage of spermatozoa shown to survive freeze-thawing -or the percentage of the parameter which survives within a group of spermatozoa -is remarkably similar in most cases, at around 40-50%.…”

Section: Quality Assays Of Spermatozoamentioning

confidence: 99%

Storage of poultry semen

Donoghue

Wishart

2000

Animal Reproduction Science

190

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Section: Quality Assays Of Spermatozoamentioning

confidence: 99%

Storage of poultry semen

Donoghue

Wishart

2000

Animal Reproduction Science

190

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“…The motilities of sperm in samples which were equilibrated in the presence of 6 4 % cryoprotectant for 15min at 4°C were 90%, 80%, 80%, 75%, 70% and 70% for glycerol, methylacetamide, EG, DMF, DMA and DMSO respectively (Ochkur, 1990). During the cryopreservation process, damage to sperm, measured as the status of coupled respiration and succinate permeability, was more the result of the change in osmolality on the addition of EG than the reduction in temperature from 41°C to 0°C (Ochkur and Kopeika, 1992a,b).…”

Section: Comparison Of Cyoprotectantsmentioning

confidence: 99%

“…The oxygen utilization characteristics of chicken sperm, in terms of degree of coupled respiration and access of substrates such as succinate, have also been used to provide evidence of plasma membrane damage during cryopreservation (Ochkur, 1990;Ochkur and Kopeika, 1992a,b).…”

Section: Sperm Quality Evaluationmentioning

confidence: 99%

Poultry artificial insemination technology in the countries of the former USSR

Surai

Wishart

1996

World's Poultry Science Journal

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A considerable volume of research on fertility in poultry, especially that associated with artificial insemination and related technologies, has been undertaken and published by scientists working in the institutes of the former USSR, now the CIS States. Researchers outside this geographical area have largely ignored this work, presumably because of the language barrier and limited accessibility of published material. That is particularly unfortunate, because in areas such as sperm cryopreservation the work of these institutes is clearly more advanced than in other countries. This paper sets out to redress the balance by reviewing artificial insemination research performed on various species of poultry, including semen extension, liquid storage and sperm cryopreservation, in the research institutes of the former USSR/CIS States.

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“…Another innovation recently considered is the addition of adenosine triphosphate (ATP) to the cryodiluent. The freeze/thaw cycle damages both avian sperm structure and function, largely the processes required for energetic maintenance including glycolysis, the citric acid cycle and the respiratory chain (Ochkur et al, 1994). Assuming that ATP depletion may occur during the re-warming phase (Holt et al, 1992) and that incorporation of exogenous ATP occurs coincident with membrane structural rearrangements, the potential of supplemental ATP improving the viability of thawed sperm seems plausible.…”

Section: Introductionmentioning

confidence: 99%

Comparative cryopreservation of avian spermatozoa: Benefits of non-permeating osmoprotectants and ATP on turkey and crane sperm cryosurvival

Blanco

Long

Gee³

et al. 2011

Animal Reproduction Science

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A comparative approach was used to evaluate the cryosurvival of turkey and crane sperm frozen in a dimethylacetamide (DMA) cryodiluent supplemented with osmoprotectants and ATP. A range (6-26%) of DMA concentrations was used alone or in combination with ATP (30, 60 or 118mM) or one of the following osmoprotectants: (1) sucrose (turkey, 8.0%; crane, 5.0%); (2) 5.0% sucrose and 5.0% trehalose; or (3) betaine hydrochloride (0.1, 0.2 or 0.4mM). The viability of thawed sperm was assessed using the nigrosin-eosin stain and sperm motility was determined using the hanging-drop technique. For semen frozen only with DMA, post-thaw sperm motility was greatest (P<0.05) for the 6.0%, 10.0% and 18% concentrations, regardless of species. Turkey sperm frozen with the sucrose/trehalose combination had greater (P<0.05) post-thaw motility for all DMA treatments compared to DMA alone. The lowest concentration of the osmoprotectant betaine hydrochloride substantially improved turkey sperm viability post-thaw in all treatments compared to DMA alone (P<0.05). The post-thaw motility of crane sperm was improved (P<0.05) with a combination of 18.0%, 24.0% or 26.0% DMA and 30mM ATP. Moreover, in the presence of osmoprotectants, crane sperm motility decreased as the osmoprotectant concentration increased. The lowest concentration of ATP also improved crane sperm viability post-thaw, especially for DMA concentrations 18% or greater. The combination of sucrose and trehalose improved (P<0.05) crane sperm viability only with 6% and 10% DMA. These data affirm that there are avian-specific differences in sperm survival after cryopreservation and suggest that post-thaw survival can be enhanced by including species-based osmoprotectant/ATP combinations in a cryodiluent where DMA is the cryoprotectant.

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The Influence of Cryopreservation on Parameters of Energetic Metabolism and Motility of Fowl Spermatozoa

Cited by 6 publications

References 0 publications

Storage of poultry semen

Storage of poultry semen

Poultry artificial insemination technology in the countries of the former USSR

Comparative cryopreservation of avian spermatozoa: Benefits of non-permeating osmoprotectants and ATP on turkey and crane sperm cryosurvival

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