The influence of cyclic tensile strain on multi-compartment collagen-GAG scaffolds for tendon-bone junction repair

Grier, William K.; Chang, Ramsay; Ramsey, Matthew D.; Harley, Brendan A.C.

doi:10.1080/03008207.2019.1601183

Cited by 25 publications

(23 citation statements)

References 70 publications

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“…Alternatively, the suspension was pipetted into Teon molds with a copper base to create anisotropic scaffolds. 46 The suspensions were cooled at a constant rate of 1 C min À1 from 20 C to À10 C. Once frozen, the suspension was held at this temperature for 2 hours then lyophilized (0.2 torr pressure, 0 C) in a VirTis Genesis 25XL Lyophilizer (SP Industries, Inc., Pennsylvania, USA).…”

Section: Fabrication Of Isotropic and Anisotropic Mineralized Collagementioning

confidence: 99%

Anisotropic mineralized collagen scaffolds accelerate osteogenic response in a glycosaminoglycan-dependent fashion

et al. 2020

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Section: Fabrication Of Isotropic and Anisotropic Mineralized Collagementioning

confidence: 99%

Anisotropic mineralized collagen scaffolds accelerate osteogenic response in a glycosaminoglycan-dependent fashion

et al. 2020

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“…The mineralized collagen suspension was transferred to 7.62 x 7.62 cm aluminum molds to create isotropic scaffolds. Alternatively, the suspension was pipetted into Teflon molds with a copper base to create anisotropic scaffolds 46 . The suspensions were cooled at a constant rate of 1°C/min from 20°C to -10°C.…”

Section: Fabrication Of Isotropic and Anisotropic Mineralized Collagementioning

confidence: 99%

Anisotropic mineralized collagen scaffolds accelerate osteogenic response in a glycosaminoglycan-dependent fashion

Dewey

Nosatov

Subedi

et al. 2020

Preprint

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Regeneration of critically-sized craniofacial bone defects requires a template to promote cell activity and bone remodeling. However, induced regeneration becomes more challenging with increasing defect size. Methods of repair using allografts and autografts have inconsistent results, attributed to age-related regenerative capabilities of bone. We are developing a mineralized collagen scaffold to promote craniomaxillofacial bone regeneration as an alternative to repair. Here, we hypothesize modifying the pore anisotropy and glycosaminoglycan content of the scaffold will improve cell migration, viability, and subsequent bone formation. Using anisotropic and isotropic scaffold variants, we test the role of pore orientation on human mesenchymal stem cell (MSC) activity. We subsequently explore the role of glycosaminoglycan content, notably chondroitin-6-sulfate, chondroitin-4-sulfate, and heparin sulfate on mineralization. We find that while short term MSC migration and activity was not affected by pore orientation, increased bone mineral synthesis was observed in anisotropic scaffolds. Further, while scaffold glycosaminoglycan content did not impact cell viability, heparin sulfate and chondroitin-6-sulfate containing variants increased mineral formation at the late stage of in vitro culture, respectively. Overall, these findings show scaffold microstructural and proteoglycan modifications represent a powerful tool to improve MSC osteogenic activity.

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“…The most common sources of naturally derived ECM used for tendon-mimetic scaffolds include small intestine submucosa, amniotic membrane [96], collagen [97], gelatin (denatured collagen) [98], glycosaminoglycans [97], silk [99], hyaluronic acid, and fibrin (described in more detail in a recent review by Freedman and Mooney [100]). Additionally, synthetic ECM has also been used for developing scaffolds for tendon repair, including poly(glycolic acid) (PGA), poly(lactic acid) (PLA), poly(ethylene glycol) (PEG) [100], poly(ε-caprolactone) (PCL) [101], and combinations of these (e.g., poly(l-lactide-co-ε-caprolactone) (PLCL) [102]), as well as polyacrylamide [100].…”

Section: Biomimetic Scaffolds For Guided Tendon Repairmentioning

confidence: 99%

Using Tools in Mechanobiology to Repair Tendons

Leek

Soulas

Sullivan

et al. 2020

Curr. Tissue Microenviron. Rep.

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Purpose of Review The purpose of this review is to describe the mechanobiological mechanisms of tendon repair as well as outline current and emerging tools in mechanobiology that might be useful for improving tendon healing and regeneration. Over 30 million musculoskeletal injuries are reported in the US per year and nearly 50% involve soft tissue injuries to tendons and ligaments. Yet current therapeutic strategies for treating tendon injuries are not always successful in regenerating and returning function of the healing tendon. Recent Findings The use of rehabilitative strategies to control the motion and transmission of mechanical loads to repairing tendons following surgical reattachment is beneficial for some, but not all, tendon repairs. Scaffolds that are designed to recapitulate properties of developing tissues show potential to guide the mechanical and biological healing of tendon following rupture. The incorporation of biomaterials to control alignment and reintegration, as well as promote scar-less healing, are also promising. Improving our understanding of damage thresholds for resident cells and how these cells respond to bioelectrical cues may offer promising steps forward in the field of tendon regeneration. Summary The field of orthopedics continues to advance and improve with the development of regenerative approaches for musculoskeletal injuries, especially for tendon, and deeper exploration in this area will lead to improved clinical outcomes.

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The influence of cyclic tensile strain on multi-compartment collagen-GAG scaffolds for tendon-bone junction repair

Cited by 25 publications

References 70 publications

Anisotropic mineralized collagen scaffolds accelerate osteogenic response in a glycosaminoglycan-dependent fashion

Anisotropic mineralized collagen scaffolds accelerate osteogenic response in a glycosaminoglycan-dependent fashion

Anisotropic mineralized collagen scaffolds accelerate osteogenic response in a glycosaminoglycan-dependent fashion

Using Tools in Mechanobiology to Repair Tendons

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